光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2015年
8期
2221-2224
,共4页
血迹%陈旧度%紫外可见反射光谱
血跡%陳舊度%紫外可見反射光譜
혈적%진구도%자외가견반사광보
Bloodstains%Age determination%Reflectance spectroscopy
凶杀案的血迹陈旧度与案发时间有着必然联系，推断案发时间对于侦破案件具有重要意义，也是司法鉴定中的一个难题，建立一种快速确定出血时间的方法非常必要。使用U V‐2450型紫外可见分光光度计，ISR‐240A积分球附件，BaSO4标准白板参比，测定16℃、湿度70％条件下血迹纱布、铝片、玻璃、保鲜膜的8 h内每间隔1 h的反射光谱，同样方法测定24℃、湿度70％条件下血迹纱布的反射光谱，利用SPSS软件处理，求出541和577 nm反射率比值（R541／R577），进行直线拟合，建立线性回归方程，进行F检验。随着时间的增加，血迹在541和577 nm处的反射率增加，除了带血迹玻璃的 R2为0.769外，其余 R2均大于0.900， F均大于F0.05（1，6）＝5.59，线性回归模型有显著意义。利用紫外可见光谱仪积分球附件可以实现一定环境载体条件下8h内血迹陈旧度推断，方法操作简单，不需要对检验对象进行任何处理，保证了其完整性，不影响对其进一步的个人信息等方面的检验，适合司法实践中的需求。
兇殺案的血跡陳舊度與案髮時間有著必然聯繫，推斷案髮時間對于偵破案件具有重要意義，也是司法鑒定中的一箇難題，建立一種快速確定齣血時間的方法非常必要。使用U V‐2450型紫外可見分光光度計，ISR‐240A積分毬附件，BaSO4標準白闆參比，測定16℃、濕度70％條件下血跡紗佈、鋁片、玻璃、保鮮膜的8 h內每間隔1 h的反射光譜，同樣方法測定24℃、濕度70％條件下血跡紗佈的反射光譜，利用SPSS軟件處理，求齣541和577 nm反射率比值（R541／R577），進行直線擬閤，建立線性迴歸方程，進行F檢驗。隨著時間的增加，血跡在541和577 nm處的反射率增加，除瞭帶血跡玻璃的 R2為0.769外，其餘 R2均大于0.900， F均大于F0.05（1，6）＝5.59，線性迴歸模型有顯著意義。利用紫外可見光譜儀積分毬附件可以實現一定環境載體條件下8h內血跡陳舊度推斷，方法操作簡單，不需要對檢驗對象進行任何處理，保證瞭其完整性，不影響對其進一步的箇人信息等方麵的檢驗，適閤司法實踐中的需求。
흉살안적혈적진구도여안발시간유착필연련계，추단안발시간대우정파안건구유중요의의，야시사법감정중적일개난제，건립일충쾌속학정출혈시간적방법비상필요。사용U V‐2450형자외가견분광광도계，ISR‐240A적분구부건，BaSO4표준백판삼비，측정16℃、습도70％조건하혈적사포、려편、파리、보선막적8 h내매간격1 h적반사광보，동양방법측정24℃、습도70％조건하혈적사포적반사광보，이용SPSS연건처리，구출541화577 nm반사솔비치（R541／R577），진행직선의합，건립선성회귀방정，진행F검험。수착시간적증가，혈적재541화577 nm처적반사솔증가，제료대혈적파리적 R2위0.769외，기여 R2균대우0.900， F균대우F0.05（1，6）＝5.59，선성회귀모형유현저의의。이용자외가견광보의적분구부건가이실현일정배경재체조건하8h내혈적진구도추단，방법조작간단，불수요대검험대상진행임하처리，보증료기완정성，불영향대기진일보적개인신식등방면적검험，괄합사법실천중적수구。
The age of bloodstains is tightly related to the time elapsed since the crime was committed .The inference of the time that the crime was committed is of great significance to solve the case ,and it was also a difficult problem in judicial authentica‐tion .Therefore ,establishing a method of rapid determination of bleeding time is very necessary .Using a UV‐visible spectrome‐ter with a reflection accessory called ISR‐240A and whiteboard as a reference standard ,the reflection spectra of blood gauze ,alu‐minum ,glass and plastic were measured every hour under the condition of 16 ℃ and 70% humidity within 8 hours .Using SPSS to process the data ,R541/R577 was figured out and linear fitting was completed .The same method was carried on on the gauze of blood at 24 ℃ .The results showed that ,within 8 hours ,spectral reflectance values of blood at 541 and 577 nm increased gradu‐ally with the passage of injury time .In addition to the R2 of the glass with blood is 0.769 ,the rest of the R2 were greater than 0.900 .The values of F were greater than F0.05 (1 ,6)=5.59 .Therefore ,the linear regression model is significant meaningful . The method of using a UV‐Visible spectrometer without doing any operations of the test samples is simple .Moreover ,it does no harm to the further inspection in aspects of personal information ,which is suitable for the judicial practice .
