CAJ | 학술논문

荧光素汞在碱性条件下具有很强的荧光，H2 S能与荧光素汞结合，使其荧光猝灭，据此建立了一种荧光法测定大鼠肠灌流液中 H2 S含量的方法。在0.1 mol · L －1 NaO H溶液中，以Na2 S作为 H2 S供体，荧光素汞浓度为5.0×10－5 mol · L －1，Na2 S浓度为1.0×10－5 mol · L －1时，以498 nm为激发波长，在522 nm处测定此二元体系的荧光强度。结果表明，在4.0×10－7～2.0×10－6 mol · L －1范围内，H2 S浓度与荧光强度的下降程度呈良好的负相关性， r＝0.9980，精密度实验RSD＝4.59％（n＝7），检出限3.5×10－8 mol · L －1，样品中H2 S含量分别为1.01×10－6和1.15×10－6 mol · L －1，加标回收率为95.8％～101.0％。该方法操作简单，灵敏度高，稳定性好，可准确测定肠灌流液中H2 S含量，为内源性H2 S的测定提供了依据。
형광소홍재감성조건하구유흔강적형광，H2 S능여형광소홍결합，사기형광졸멸，거차건립료일충형광법측정대서장관류액중 H2 S함량적방법。재0.1 mol · L －1 NaO H용액중，이Na2 S작위 H2 S공체，형광소홍농도위5.0×10－5 mol · L －1，Na2 S농도위1.0×10－5 mol · L －1시，이498 nm위격발파장，재522 nm처측정차이원체계적형광강도。결과표명，재4.0×10－7～2.0×10－6 mol · L －1범위내，H2 S농도여형광강도적하강정도정량호적부상관성， r＝0.9980，정밀도실험RSD＝4.59％（n＝7），검출한3.5×10－8 mol · L －1，양품중H2 S함량분별위1.01×10－6화1.15×10－6 mol · L －1，가표회수솔위95.8％～101.0％。해방법조작간단，령민도고，은정성호，가준학측정장관류액중H2 S함량，위내원성H2 S적측정제공료의거。
Under alkaline conditions ,Fluorescein mercury has strong fluorescence ,however ,when it met S2 - ,its fluorescence would quench ,in view of the above ,a fluorescence method for determination of H2S in biological samples was established .In the 0.1 mol · L -1 NaOH dilution ,when the concentration of fluorescein Mercury and Na2 S was 5.0 × 10-5 and 1.0 × 10-5 mol · L -1 respectively ,the fluorescence intensity of system was determined at 522 nm .The results showed that ,at the range of 4.0 × 10-7 ~2.0 × 10-6 mol · L -1 ,the concentration decreasing of H2 S and fluorescence intensity had good linear relationship , r=0.998 0 ,the RSD of precision test was 4.59% (n=7) ,the detection limit was 3.5 × 10-8 mol · L -1 ,the content of H2 S in the sample were 1.01 × 10-6 and 1.15 × 10-6 mol · L -1 ,and the recovery rate was 95.8% ~101.0% ,the method has the advanta‐ges of simple operation ,high sensitivity ,good selectivity ,can accurately determine of H 2 S in intestinal perfused solution ,and provides the basis for the determination of endogenous H 2 S .