CAJ | 학술논문

硫酸软骨素的测定在生物医学领域有重要价值，但常规检测法在灵敏度、选择性或简易性方面尚存在不足。本文基于带正电基团的阳离子表面活性剂对具有红区发射特性的强荧光化合物—荷负电的四磺基铝酞菁具有高效荧光猝灭作用，而在生物多糖硫酸软骨素存在下，上述荧光猝灭体系荧光显著恢复的现象，提出酞菁‐表面活性剂离子缔合物荧光恢复高灵敏测定硫酸软骨素的新方法，并用于实际样品分析。研究表明，中性介质中，红区荧光探针四磺基铝酞菁（Tetrasulfonated aluminium phthalocyanine ，AlS4 Pc）与阳离子表面活性剂十四烷基二甲基苄基氯化铵（Tetradecyldimethylbenzylammonium chloride ，TDBAC）发生强烈的缔合作用，导致AlS4 Pc的荧光几乎完全猝灭，从而获得暗背景的荧光体系。在加入带有阴离子基团（磺酸基）的生物多糖硫酸软骨素（Chondroitin sulfate ，CS）后，由于竞争结合作用，AlS4 Pc被释放而使体系的荧光大幅度恢复，且恢复程度与CS呈线性正相关。优化了反应条件，考察了共存物质的影响，结果表明本法具有较好的选择性。在最佳条件下，线性范围为0.20～10.0μg · m L －1，检测限为0.070μg · m L －1，工作曲线方程 y＝1.04 x＋2.09， r＝0.9995。该法操作简便，灵敏度、稳定性与准确性好，实际样品的分析结果令人满意。酞菁荧光化合物在分析科学中的应用尚不多见，本文工作进一步开拓了酞菁红区荧光探针的新应用。
류산연골소적측정재생물의학영역유중요개치，단상규검측법재령민도、선택성혹간역성방면상존재불족。본문기우대정전기단적양리자표면활성제대구유홍구발사특성적강형광화합물—하부전적사광기려태정구유고효형광졸멸작용，이재생물다당류산연골소존재하，상술형광졸멸체계형광현저회복적현상，제출태정‐표면활성제리자체합물형광회복고령민측정류산연골소적신방법，병용우실제양품분석。연구표명，중성개질중，홍구형광탐침사광기려태정（Tetrasulfonated aluminium phthalocyanine ，AlS4 Pc）여양리자표면활성제십사완기이갑기변기록화안（Tetradecyldimethylbenzylammonium chloride ，TDBAC）발생강렬적체합작용，도치AlS4 Pc적형광궤호완전졸멸，종이획득암배경적형광체계。재가입대유음리자기단（광산기）적생물다당류산연골소（Chondroitin sulfate ，CS）후，유우경쟁결합작용，AlS4 Pc피석방이사체계적형광대폭도회복，차회복정도여CS정선성정상관。우화료반응조건，고찰료공존물질적영향，결과표명본법구유교호적선택성。재최가조건하，선성범위위0.20～10.0μg · m L －1，검측한위0.070μg · m L －1，공작곡선방정 y＝1.04 x＋2.09， r＝0.9995。해법조작간편，령민도、은정성여준학성호，실제양품적분석결과령인만의。태정형광화합물재분석과학중적응용상불다견，본문공작진일보개탁료태정홍구형광탐침적신응용。
Determination of chondroitin sulfate in the biomedical field has an important value .The conventional methods for the assay of chondroitin sulfate are still unsatisfactory in sensitivity ,selectivity or simplicity .This work aimed at developing a novel method for sensitive and selective determination of chondroitin sulfate by fluorimetry .We found that some kinds of cationic sur‐factants have the ability to quench the fluorescence of tetrasulfonated aluminum phthalocyanine (AlS4 Pc) ,a strongly fluorescent compound which emits at red region ,with high efficiency .But ,the fluorescence of the above‐mentioned fluorescence quenching system recovered significantly when chondroitin sulfate (CS) exits .Tetradecyl dimethyl benzyl ammonium chloride(TDBAC) which was screened from all of the candidates of cationic surfactants was chosen as the quencher because it shows the most effi‐cient quenching effect .It was found that the fluorescence of AlS4 Pc was extremely quenched by TDBAC because of the formation of association complex between AlS4 Pc and TDBAC .Fluorescence of the association complex recovered dramatically after the ad‐dition of chondroitin sulfate (CS) due to the ability of chondroitin sulfate to shift the association equilibrium of the association , leading to the release of AlS4 Pc ,thus resulting in an increase in the fluorescence of the reaction system .Based on this phenome‐non ,a novel method with simplicity ,accuracy and sensitivity was developed for quantitative determination of CS .Factors inclu‐ding the reaction time ,influencing factors and the effect of coexisting substances were investigated and discussed .Under opti‐mum conditions the linear range of the calibration curve was 0 .20~10 .0μg · mL -1 .The detection limit for CS was 0 .070 μg · mL -1 .The method has been applied to the analysis of practical samples with satisfied results .This work expands the applica‐tions of AlS4 Pc in biomedical area .