中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2015年
7期
497-500
,共4页
邓振领%张万久%韩玥%张叔人
鄧振領%張萬久%韓玥%張叔人
산진령%장만구%한모%장숙인
乳腺肿瘤%消融技术%肿瘤转移%小鼠%免疫系统
乳腺腫瘤%消融技術%腫瘤轉移%小鼠%免疫繫統
유선종류%소융기술%종류전이%소서%면역계통
Breast neoplasms%Ablation techniques%Neoplasm metastasis%Mice%Immune system
目的：探讨射频消融术对小鼠三阴性乳腺癌肺转移和免疫系统的影响。方法小鼠乳腺癌4T1细胞系皮下接种于雌性Bal B／c小鼠右下肢处，肿瘤直径达到6～8 mm时进行射频消融治疗，流式细胞术检测脾脏中各淋巴细胞亚群的变化，观察肺转移的情况。结果射频消融基本上可以消除原位肿瘤，原位肿瘤复发率较低；射频消融后，小鼠脾脏CD4＋T细胞、CD8＋T细胞、B细胞、自然杀伤细胞及自然杀伤T细胞数量都有所升高；射频消融治疗4T1肿瘤14 d后，脱颈处死对照组和射频消融组小鼠，射频消融组和对照组4T1荷瘤小鼠的肺转移结节分别为（24±18）个和（81±35）个，差异有统计学意义（ P＝0．012）。射频消融抑制肺转移的机制与脾脏中CD4＋T细胞、CD8＋T细胞、B细胞和自然杀伤细胞的比例增加及髓系来源抑制细胞比例降低有关。结论射频消融增强了机体内抗肿瘤的免疫反应，抑制了小鼠4T1细胞乳腺癌的肺转移。射频消融术治疗三阴性乳腺癌和控制其远端转移可能会有良好的治疗效果。
目的：探討射頻消融術對小鼠三陰性乳腺癌肺轉移和免疫繫統的影響。方法小鼠乳腺癌4T1細胞繫皮下接種于雌性Bal B／c小鼠右下肢處，腫瘤直徑達到6～8 mm時進行射頻消融治療，流式細胞術檢測脾髒中各淋巴細胞亞群的變化，觀察肺轉移的情況。結果射頻消融基本上可以消除原位腫瘤，原位腫瘤複髮率較低；射頻消融後，小鼠脾髒CD4＋T細胞、CD8＋T細胞、B細胞、自然殺傷細胞及自然殺傷T細胞數量都有所升高；射頻消融治療4T1腫瘤14 d後，脫頸處死對照組和射頻消融組小鼠，射頻消融組和對照組4T1荷瘤小鼠的肺轉移結節分彆為（24±18）箇和（81±35）箇，差異有統計學意義（ P＝0．012）。射頻消融抑製肺轉移的機製與脾髒中CD4＋T細胞、CD8＋T細胞、B細胞和自然殺傷細胞的比例增加及髓繫來源抑製細胞比例降低有關。結論射頻消融增彊瞭機體內抗腫瘤的免疫反應，抑製瞭小鼠4T1細胞乳腺癌的肺轉移。射頻消融術治療三陰性乳腺癌和控製其遠耑轉移可能會有良好的治療效果。
목적：탐토사빈소융술대소서삼음성유선암폐전이화면역계통적영향。방법소서유선암4T1세포계피하접충우자성Bal B／c소서우하지처，종류직경체도6～8 mm시진행사빈소융치료，류식세포술검측비장중각림파세포아군적변화，관찰폐전이적정황。결과사빈소융기본상가이소제원위종류，원위종류복발솔교저；사빈소융후，소서비장CD4＋T세포、CD8＋T세포、B세포、자연살상세포급자연살상T세포수량도유소승고；사빈소융치료4T1종류14 d후，탈경처사대조조화사빈소융조소서，사빈소융조화대조조4T1하류소서적폐전이결절분별위（24±18）개화（81±35）개，차이유통계학의의（ P＝0．012）。사빈소융억제폐전이적궤제여비장중CD4＋T세포、CD8＋T세포、B세포화자연살상세포적비례증가급수계래원억제세포비례강저유관。결론사빈소융증강료궤체내항종류적면역반응，억제료소서4T1세포유선암적폐전이。사빈소융술치료삼음성유선암화공제기원단전이가능회유량호적치료효과。
Objective To explore the effects of radiofrequency ablation( RFA) on immune system and lung metastasis in a mouse model of triple negative breast cancer 4T1. Methods Mouse breast cancer 4T1 cells were injected into the right hind limb of female Bal B/c mice. When the tumor size was 6?8 mm in diameter, RFA was used to treat the transplanted breast cancer in mice. We examined the splenic lymphocyte subsets by flow cytometry at different time points after RFA. Fourteen days after treatment, we sacrificed the mice of both control and treatment groups, counted the number of lung metastatic nodules, and detected the changes of splenic lymphocyte subsets by flow cytometry. Results RFA basically eliminated the orthotopic carcinoma with a low local recurrence rate. After the RFA treatment, the amount of spleic CD4+T cells,CD8+T cells, B cells, NK and NKT cells was increased. Fourteen days after the RFA treatment, all mice were sacrificed, and the lung metastatic nodules were 24±18 in the control group and 81±35 in the RFA?treated group ( P=0.012) . The mechanism of suppression of metastatic lung cancers was related to the increase of splenic CD4+ T cells, CD8+T cells, B cells and NK cells, and the decrease of myeloid?derived suppressor cells. Conclusions RFA can enhance the anti?tumor immunity and effectively inhibit lung metastasis of 4T1 cell?induced breast cancer, and has a good potential effect in the treatment of triple?negative breast cancer and the control of distant metastasis.
