CAJ | 학술논문

研究人参皂苷分子与生物膜的作用对于深入了解中药人参的药理活性及其生物学功效至关重要。DPPC作为具有双分子层结构的脂质分子，常被许多国内外学者作为模拟膜的模型来研究药物分子与细胞膜的作用；Rb1作为中药人参中的重要皂苷成分，具有显著的药理学功效和生物性能。拉曼光谱是探讨分子间作用的有力工具，差示扫描量热技术（differential scanning calorimetry ，DSC）是研究脂双层分子单体及其与药物分子作用的常用技术，而将两者结合研究药物分子对细胞膜作用的研究的报道较少。本文采用变温拉曼光谱和DSC探讨了在温度变化条件下人参皂苷Rb1单体分子与DPPC双层膜的作用。通过拉曼光谱测试，在Rb1作用前后，DPPC分子极性头部O—C—C—N＋和C—C伸缩振动区域以及烷基链部分C—H键的伸缩振动区域的变化表明，随着温度的增加，含有一定浓度Rb1的DPPC磷脂极性头部旁氏构象没有发生变化，脂酰链的无序性构象增多，侧向排列的无序性增强，DPPC脂双层的流动性增加。由DSC实验得到的几个热力学常数[相变温度（ Tm ）、半峰宽（ΔT1／2）及相转变焓值（ΔH ）]的变化表明，DSC进一步验证了变温拉曼实验结果，随着Rb1浓度的增大，DPPC双层膜的相变温度显著下降，流动性增强，说明Rb1对DP‐PC双层膜的影响较大。
연구인삼조감분자여생물막적작용대우심입료해중약인삼적약리활성급기생물학공효지관중요。DPPC작위구유쌍분자층결구적지질분자，상피허다국내외학자작위모의막적모형래연구약물분자여세포막적작용；Rb1작위중약인삼중적중요조감성분，구유현저적약이학공효화생물성능。랍만광보시탐토분자간작용적유력공구，차시소묘량열기술（differential scanning calorimetry ，DSC）시연구지쌍층분자단체급기여약물분자작용적상용기술，이장량자결합연구약물분자대세포막작용적연구적보도교소。본문채용변온랍만광보화DSC탐토료재온도변화조건하인삼조감Rb1단체분자여DPPC쌍층막적작용。통과랍만광보측시，재Rb1작용전후，DPPC분자겁성두부O—C—C—N＋화C—C신축진동구역이급완기련부분C—H건적신축진동구역적변화표명，수착온도적증가，함유일정농도Rb1적DPPC린지겁성두부방씨구상몰유발생변화，지선련적무서성구상증다，측향배렬적무서성증강，DPPC지쌍층적류동성증가。유DSC실험득도적궤개열역학상수[상변온도（ Tm ）、반봉관（ΔT1／2）급상전변함치（ΔH ）]적변화표명，DSC진일보험증료변온랍만실험결과，수착Rb1농도적증대，DPPC쌍층막적상변온도현저하강，류동성증강，설명Rb1대DP‐PC쌍층막적영향교대。
The research on the interactions between Ginsenosides and biomembranes plays a crucial role in thorough understand‐ing the pharmacological activity and biologyical effect of Chinese medicine Panax ginseng .With the bilayer structure ,DPPC of‐ten serves as an simulation model of the cell membrane to study the role of drug molecules and cell membranes .Ginsenoside Rb1 ,one of the most important components of Panaxginseng ,playing the significant roles of pharmacological effects and biologi‐cal properties .Raman and differential scanning calorimetry(DSC) are respectively a powerful tool for discussing the molecular in‐teraction ,and a kind of general technology by which researching the bilayer monomer structures and its interactions with drug molecules .However ,rarely research reports on the interactions between drug molecules and biomembranes by means of both technologies above .In this paper ,the influence of ginsenoside monomer Rb1 on DPPC membrane bilayers was investigated by thermo‐Raman and DSC .In Raman spectra ,the changes of DPPC molecule have been observed before and after interacted with ginsenoside Rb1 ,the data analysis indicates three aspects :the O—C—C—N+ polar head group skeleton ,C—C stretching vi‐bration area ,and the C—H bond stretching vibrarion in terminated methyl group of alkyl chains .The results showed that gin‐senoside Rb1 molecule with certain concentration has not changed the gauche conformation of the polar head backbone group in DPPC bilayers ,the order of the internal molecular chain and the lateral chain‐chain packing have been decreased as the tempera‐ture increased ,the lateral disposed disorder has been increased .The changes of some thermodynamic constants obtained by DSC experiment such as phase transition temperature (Tm )、the temperature at which the transition is half completed (ΔT1/2 ) ,and the transition enthalpy normalized per mol of DPPC (ΔH) have been showed further results of the thermo Raman experiments , with increasing the concentration of ginsenoside Rb1 ,the pre‐transition temperature of DPPC bilayers dropped immediately with small amount of the Rb1 drug when the containtion was only 5 mol% and the whole system has been destructed at the same time ,the main phase transition peak showed as a new little shoulder seam ,however ,both pre‐and main transition peak disap‐peared completely until the drug concentration increased to 20 mol% ,the phase transition temperature of DPPC has been reduced significantly ,and the fluidity of bilayers has been increased .Both experiments indicated that the strong effects of ginsenoside Rb1 on DPPC .