CAJ | 학술논문

目的：以BALB/c荷瘤小鼠为对象，探讨纳秒级陡脉冲电场对在体小鼠乳腺癌的杀伤效应。方法取24只皮下接种小鼠乳腺癌细胞EMT-6的BALB/c荷瘤鼠，分为实验组和对照组，每组12只。在麻醉状态下，采用场强为700 V/cm、脉宽为70μs、上升时间为57 ns、占空比为50%的陡脉冲电场，利用自制的电极针插入实验组小鼠肿瘤两端进行电刺激处理，脉冲个数为100个，能量为9.75 J，有效作用时间仅为7 ms。对照组不进行电刺激，其他处理相同。隔天测量动物体质量、肿瘤大小，并在处理后第1、3、5、7天分别取肿瘤组织做病理切片。结果与对照组相比，实验组小鼠肿瘤生长速度较慢，特别在处理后1~3d内肿瘤几乎停止生长。组织病理结果显示电刺激处理后肿瘤组织有明显的凝固性坏死表现，组织水肿明显，处理后第3天肿瘤组织中出现大量空泡样结构，血管损伤在早期即出现。结论在给定电场条件下，陡脉冲电场对肿瘤生长有一定的抑制作用。要想达到更理想的肿瘤治疗效果，需对陡脉冲剂量进行进一步的研究。
목적：이BALB/c하류소서위대상，탐토납초급두맥충전장대재체소서유선암적살상효응。방법취24지피하접충소서유선암세포EMT-6적BALB/c하류서，분위실험조화대조조，매조12지。재마취상태하，채용장강위700 V/cm、맥관위70μs、상승시간위57 ns、점공비위50%적두맥충전장，이용자제적전겁침삽입실험조소서종류량단진행전자격처리，맥충개수위100개，능량위9.75 J，유효작용시간부위7 ms。대조조불진행전자격，기타처리상동。격천측량동물체질량、종류대소，병재처리후제1、3、5、7천분별취종류조직주병리절편。결과여대조조상비，실험조소서종류생장속도교만，특별재처리후1~3d내종류궤호정지생장。조직병리결과현시전자격처리후종류조직유명현적응고성배사표현，조직수종명현，처리후제3천종류조직중출현대량공포양결구，혈관손상재조기즉출현。결론재급정전장조건하，두맥충전장대종류생장유일정적억제작용。요상체도경이상적종류치료효과，수대두맥충제량진행진일보적연구。
Objective To explore the in vivo killing effects of nanosecond pulsed electric fields on mouse breast cancer in tumor bearing BALB/c mice. Methods Twenty four BALB/c mice models with subcutaneous EMT-6 breast cancer were prepared and divided into experimental group and control group (n=12). In experimental group, the mice were treated by the pulsed electric fields with peak voltage of 700 V/cm, rise times of 57 ns, durations of 70μs and duty ratio of 50%on the tumor tissue with two parallel electrodes under anaesthesia. One hundred pulses were given and the energy was just 9.75 J with effective action period of 7 ms. Tumor volume, animal weight and histopathologic changes after 1, 3, 5 and 7 d were observed after irreversible electroporation. Results The tumor volume in experimental group increased more slowly than that in control group, especially at the 1-3 d after treatment with a standstill. Histopathological examination results showed that the tumor tissue had obvious coagulation necrosis and tissue edema after electrical stimulation treatment, and mass vacuole like structure appeared in tumor tissue from the third day after treatment and vascular injury occurred at the early stage. Conclusions In the given field conditions, steep pulsed electric field has inhibitory effect on tumor growth. In order to achieve more ideal tumor therapy effect, further studies on steep pulse dose are needed.