CAJ | 학술논문

目的：探索人胎盘间充质干细胞（MSCs）分化为胰岛素分泌细胞的诱导条件。方法从胎盘组织中获取MSCs，采用激活素A、表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）等多种细胞因子诱导胎盘MSCs向胰岛素分泌细胞分化。利用免疫细胞化学染色、Western Blot及动物实验，对诱导后的细胞进行鉴定。结果经诱导后的细胞具备胰岛β细胞的部分特征，能表达胰十二指肠同源盒-1（PDX-1）、胰岛素和C肽，对糖尿病小鼠具有降糖作用。结论人胎盘MSCs经多种细胞因子诱导后可分化为胰岛素分泌细胞。
목적：탐색인태반간충질간세포（MSCs）분화위이도소분비세포적유도조건。방법종태반조직중획취MSCs，채용격활소A、표피생장인자（EGF）、감성성섬유세포생장인자（bFGF）등다충세포인자유도태반MSCs향이도소분비세포분화。이용면역세포화학염색、Western Blot급동물실험，대유도후적세포진행감정。결과경유도후적세포구비이도β세포적부분특정，능표체이십이지장동원합-1（PDX-1）、이도소화C태，대당뇨병소서구유강당작용。결론인태반MSCs경다충세포인자유도후가분화위이도소분비세포。
Objective To explore the methods of inducing human placenta mesenchymal stem cells (MSCs) to differentiate into insulin-producing cells. Methods MSCs were obtained from human placenta and were induced to differentiate into insulin-producing cells by activin A, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and other cytokines. The cells were identified by immunocytochemistry staining, Western Blot and animal experiment after differentiation. Results After induction, the cells possessed some characteristics of islet βcells, and could express pancreatic duodenal homeobox-1 (PDX-1), insulin and C peptide. The induced cells could also decrease blood glucose levels of diabetic mice. Conclusions These results showed that human placenta MSCs can be induced to differentiate into insulin-producing cells by cytokines.