CAJ | 학술논문

目的：探讨甘草功能基因拷贝数多态性（CNV）与叶片形态特征及甘草酸含量的相关性，为筛选高品质甘草奠定基础。方法：以来自7个产地的60株甘草为实验材料，采用实时PCR对甘草中的3-羟基-3-甲基戊二酰CoA还原酶（HMGR）基因、鲨稀合成酶1（SQS1）基因和β-香树脂醇合成酶（β-AS）基因进行拷贝数测定，采用HPLC测定甘草酸含量，并考察不同功能基因CNV组合类型甘草叶片的形态特征。结果：根据实时PCR测定结果，按照3个功能基因的拷贝数，将甘草分为6种类型，即A型（β-AS+HMGR+SQS1=1+1+1）、B型（1+2+1）、C型（2+1+1）、D型（2+1+2）、E型（2+2+1）和F型（2+2+2）；不同类型甘草在叶片形态特征方面存在显著性差异，A型与D型叶片的整体面积较小，而B型与E型叶片的整体面积则较大；HPLC测定结果显示B型甘草的甘草酸平均含量最高，E型甘草的甘草酸含量较高，而A型和D型的甘草酸含量则较低。结论：甘草中功能基因CNV组合类型与甘草叶片的形态特征及甘草酸含量之间存在明确的相关性，β-AS基因与SQS1基因单拷贝、HMGR基因双拷贝的甘草植株，其叶片具有最大的面积，同时其甘草酸含量最高。本研究结果可为优质甘草筛选奠定基础。
목적：탐토감초공능기인고패수다태성（CNV）여협편형태특정급감초산함량적상관성，위사선고품질감초전정기출。방법：이래자7개산지적60주감초위실험재료，채용실시PCR대감초중적3-간기-3-갑기무이선CoA환원매（HMGR）기인、사희합성매1（SQS1）기인화β-향수지순합성매（β-AS）기인진행고패수측정，채용HPLC측정감초산함량，병고찰불동공능기인CNV조합류형감초협편적형태특정。결과：근거실시PCR측정결과，안조3개공능기인적고패수，장감초분위6충류형，즉A형（β-AS+HMGR+SQS1=1+1+1）、B형（1+2+1）、C형（2+1+1）、D형（2+1+2）、E형（2+2+1）화F형（2+2+2）；불동류형감초재협편형태특정방면존재현저성차이，A형여D형협편적정체면적교소，이B형여E형협편적정체면적칙교대；HPLC측정결과현시B형감초적감초산평균함량최고，E형감초적감초산함량교고，이A형화D형적감초산함량칙교저。결론：감초중공능기인CNV조합류형여감초협편적형태특정급감초산함량지간존재명학적상관성，β-AS기인여SQS1기인단고패、HMGR기인쌍고패적감초식주，기협편구유최대적면적，동시기감초산함량최고。본연구결과가위우질감초사선전정기출。
Objective: To investigate the relationship between the functional gene copy number variations(CNV), the leaf morphological characteristics and the content levels of glycyrrhizic acid in Glycyrrhiza uralensis. Methods:Sixty G.uralensis plants from seven origins were used as materials. The copy number of 3 functional genes, HMGR, SQS1 and β-AS, were determined by real-time PCR, and glycyrrhizic acid contents were analyzed by HPLC. The leaf morphological characteristics of them were also analyzed. Results: Based on the results of real-time PCR, the 60 G.uralensis samples were divided into group A(β-AS+HMGR+SQS1=1+1+1), B(1+2+1), C(2+1+1), D(2+1+2), E(2+2+1) and F(2+2+2). There were significant differences between the leaf morphological characteristics in differ?ent groups, and the blade area of samples in group B and E were bigger. Among the six groups, group B and E contained relatively higher contents of glycyrrhizic acid. Conclusion: There was a definite relationship between the functional gene CNV, the leaf morphological characteristics and the content levels of glycyrrhizic acid in G.uralen?sis. The samples with one copy of β-AS and SQS1 genes, two copies of HMGR gene had the biggest blade area and contained relatively highest contents of glycyrrhizic acid. The conclusion of this paper will lay a foundation for screening high-quality G.uralensis.