CAJ | 학술논문

目的：分析培养基、外源激素和真菌诱导子对北柴胡毛状根生长和柴胡皂苷含量的影响，筛选出柴胡皂苷产量高的培养条件。方法：设置3种培养基（B5、改良White和WPM）、3种外源激素（IBA、IAA和NAA）、真菌诱导子为黑曲霉诱导子，每组处理设计2~4个浓度梯度，25℃、110 r/min暗培养，3个月后收获称重，测定根产量，HPLC方法检测毛状根柴胡皂苷含量。结果：在添加0.5 mg/L IBA的B5培养基中，毛状根和柴胡皂苷产量最高，分别为2.4 g和9.05 mg。结论：筛选出北柴胡毛状根最适培养条件，为北柴胡毛状根大规模培养利用提供了参考依据。
목적：분석배양기、외원격소화진균유도자대북시호모상근생장화시호조감함량적영향，사선출시호조감산량고적배양조건。방법：설치3충배양기（B5、개량White화WPM）、3충외원격소（IBA、IAA화NAA）、진균유도자위흑곡매유도자，매조처리설계2~4개농도제도，25℃、110 r/min암배양，3개월후수획칭중，측정근산량，HPLC방법검측모상근시호조감함량。결과：재첨가0.5 mg/L IBA적B5배양기중，모상근화시호조감산량최고，분별위2.4 g화9.05 mg。결론：사선출북시호모상근최괄배양조건，위북시호모상근대규모배양이용제공료삼고의거。
Objective: To study the effects of different media, phytohormones and fungus elicitors on the growth and saikosaponin production of Bupleurum chinense DC. hairy roots. Methods: Conditions were screened including three different culture media(B5, modified-White and WPM), three types of exogenous hormones(IBA, IAA and NAA) and fugus elicitor from Aspergillus niger. Two to four concentration gradients were set for each group. The culture condition was at 25℃, 110 r/min. After three months, the weights of roots were measured and the saikosa?ponin content were determined by HPLC method. Results: The production of roots and saikosaponin were highest in the B5 culture medium added with 0.5 mg/L IBA, those were 2.4 g and 9.05 mg, respectively. Conclusion: The optimal condition for saikosaponin production from hair roots which we screened out will provide a basis for fur?ther cultivation in large scales.