CAJ | 학술논문

目的：利用荧光反射成像技术，重建出小鼠下肢外周组织的灌注参数的分布图像。方法BALB/c裸鼠尾静脉注射10μg的吲哚菁绿（ICG）溶液，给药后立即用荧光反射成像系统连续拍摄ICG荧光强度图像，总计时长200 s。随后拍取小鼠轮廓的白光图，以获得感兴趣区域（ROI）。用双指数模型对ROI区域的动态荧光数据建模分析，重建出小鼠下肢外周组织的灌注参数图像。结果利用双指数模型获得的荧光强度-时间曲线与测量的灌注曲线接近，并重建出反映小鼠下肢外周组织血液灌注情况的参数分布图。结论提出一种量化分析小鼠下肢外周组织血液灌注状态的方法，重建的灌注参数图像分辨率较高。该方法采用非入侵式活体成像，对实验对象损伤小。
목적：이용형광반사성상기술，중건출소서하지외주조직적관주삼수적분포도상。방법BALB/c라서미정맥주사10μg적신타정록（ICG）용액，급약후립즉용형광반사성상계통련속박섭ICG형광강도도상，총계시장200 s。수후박취소서륜곽적백광도，이획득감흥취구역（ROI）。용쌍지수모형대ROI구역적동태형광수거건모분석，중건출소서하지외주조직적관주삼수도상。결과이용쌍지수모형획득적형광강도-시간곡선여측량적관주곡선접근，병중건출반영소서하지외주조직혈액관주정황적삼수분포도。결론제출일충양화분석소서하지외주조직혈액관주상태적방법，중건적관주삼수도상분변솔교고。해방법채용비입침식활체성상，대실험대상손상소。
Objective To reconstruct the perfusion parameters of murine hindlimbs peripheral tissue by using fluorescence reflectance imaging technique. Methods BALB/c mice were injected with intravenous bolus injection of indocyanine green (ICG) (10μg) into the tail vein. Time-series fluorescence intensity images were obtained for 200 s immediately after the injection. After the serial imaging, silhouette images of the mice were taken under white light to obtain the region of interest (ROI) of the murine hindlimbs. Bi-exponential model was applied to analyze the dynamic fluorescence parameters and the peripheral tissue perfusion parameter images were reconstructed. Results The fitted perfusion curves obtained from bi-exponential model were in good agreement with the measured ones. The parametric images which reflected the vascular sufficiency of murine hindlimbs were reconstructed. Conclusions A novel method for parametric images of murine hindlimbs peripheral tissue blood perfusion is proposed in this paper, which is noninvasive with higher resolution and little damage to biological tissues.