CAJ | 학술논문

目的：探讨肝细胞肝癌患者血浆microRNA－21（ miR－21）水平的变化及其临床意义。方法病例对照研究，采用实时荧光定量PCR检测河南省人民医院2014年1至6月收治的60例肝细胞肝癌（ HCC）患者、71例肝硬化（ LC）患者及52名健康体检者（ HV）血浆miR－21表达量并计算miR－21相对表达量。通过分析受试者工作特征曲线（ ROC）判断miR－21表达水平在肝癌诊断中的敏感度和特异度。两样本均数比较采用t检验。结果 HCC组患者血浆miR－21相对表达量（2．6±1．1）均高于LC组（1．6±0．9）和HV组（1．0±0．6），差异有统计学意义（HCC组与LC组比较t＝5．322，P＝0．004；HCC组与HV组比较t＝8．349，P＝0．0003）。 HCC患者血浆miR－21相对表达量与肿瘤分化和大小相关，差异有统计学意义（tdif ＝3．366，P＝0．019；tsize ＝3．490，P＝0．012），与TNM肿瘤分期、肿瘤数量、AFP浓度无相关，差异无统计学意义（ tTNM ＝1．103，P＝0．274；tnum ＝1．682，P＝0．097；tAFP ＝1．756，P＝0．084）。 ROC分析miR－21相对表达量在HCC组与HV组比较中敏感度为89．5％，特异度为81．8％，曲线下面积（ AUC）为0．934；miR－21在HCC组与LC组比较中敏感度为70．0％，特异度为65．3％，AUC为0．796。结论肝细胞肝癌患者血浆中miR－21高表达，可能对肝细胞肝癌的临床诊断有意义。（中华检验医学杂志，2015，38：484－486）
목적：탐토간세포간암환자혈장microRNA－21（ miR－21）수평적변화급기림상의의。방법병례대조연구，채용실시형광정량PCR검측하남성인민의원2014년1지6월수치적60례간세포간암（ HCC）환자、71례간경화（ LC）환자급52명건강체검자（ HV）혈장miR－21표체량병계산miR－21상대표체량。통과분석수시자공작특정곡선（ ROC）판단miR－21표체수평재간암진단중적민감도화특이도。량양본균수비교채용t검험。결과 HCC조환자혈장miR－21상대표체량（2．6±1．1）균고우LC조（1．6±0．9）화HV조（1．0±0．6），차이유통계학의의（HCC조여LC조비교t＝5．322，P＝0．004；HCC조여HV조비교t＝8．349，P＝0．0003）。 HCC환자혈장miR－21상대표체량여종류분화화대소상관，차이유통계학의의（tdif ＝3．366，P＝0．019；tsize ＝3．490，P＝0．012），여TNM종류분기、종류수량、AFP농도무상관，차이무통계학의의（ tTNM ＝1．103，P＝0．274；tnum ＝1．682，P＝0．097；tAFP ＝1．756，P＝0．084）。 ROC분석miR－21상대표체량재HCC조여HV조비교중민감도위89．5％，특이도위81．8％，곡선하면적（ AUC）위0．934；miR－21재HCC조여LC조비교중민감도위70．0％，특이도위65．3％，AUC위0．796。결론간세포간암환자혈장중miR－21고표체，가능대간세포간암적림상진단유의의。（중화검험의학잡지，2015，38：484－486）
Objective To evaluate the plasma level of miR-21 in hepatocellular carcinoma (HCC) patients and its clinical diagnostic significance .Methods Case-control study was used .The plasma level of miR-21 was measured by real-time quantitative PCR.The relative expressions of miR-21 were calculated. This study included 60 cases of patients with HCC , 71 patients with liver cirrhosis ( LC ) , 52 healthy volunteers ( HV) from January to June in 2014 in Henan Province People′s Hospital.The receiver operating characteristic ( ROC) curves were analyzed to determine the sensitivity and specificity of miR-21 expression levels in HCC diagnosis. Differences between groups were assessed by the t-test.Results Plasma microRNA-21 level in the 60 patients with hepatocellular carcinoma ( 2.6 ±1.1 ) was significantly higher than in patients with chronic hepatitis (1.6 ±0.9) and healthy volunteers (1.0 ±0.6) (t=5.322,P=0.004;t =8.349, P =0.000 3, respectively ) .Plasma microRNA-21 level in the HCC patients were positively correlated with tumor size and differentiation (tdif=3.366,P=0.019;tsize =3.490,P=0.012). ROC analysis of plasma microRNA-21 yielded an AUC of 0.796 with 70.0% sensitivity and 65.3%specificity when differentiating hepatocellular carcinoma from chronic hepatitis , and an AUC of 0.934 with 89.5% sensitivity and 81.8% specificity when differentiating hepatocellular carcinoma from healthy volunteers.Conclusion The plasma level of miR-21 in HCC patients has high specificity , and maybe help to diagnose of HCC.