CAJ | 학술논문

目的：探讨血红蛋白变异体对糖化血红蛋白（ HbA1c ）不同检测系统的影响。方法采用酶法（Norudia N HbA1c，SEKISUI）、免疫透射比浊法（oneHbA1c FS，DiaSys）、亲和层析高压液相色谱法（Ultra2，Trinity Biotech）、毛细管电泳法[Capillary 2 Flex Piercing（CFP）Sebia]和离子交换高压液相色谱法[HA8160，Arkary；VariantⅡTurbo（Ⅶ－T），Bio－Rad；VariantⅡTurbo 2．0（Ⅶ－T 2．0），Bio－Rad]7种检测系统测定正常样本和血红蛋白变异体样本HbA1c ，分析血红蛋白变异体对HbA1c检测结果的影响。结果对于正常样本，7种检测系统的相关性良好。对于（ HbE）杂合子，以（ CFP）为参比系统，Ⅶ－T的HbA1c结果分成高低两群，各检测系统的HbA1c结果超过6％比例范围为14．9％～73．1％。对于HbE纯合子，Ultra2检测出部分样本的糖化血红蛋白结果，CFP和Ⅶ－T 2．0未给出HbA1c结果。常见血红蛋白变异体中，J－Bangkok、Hb J－Bangkok、Hb J－Newyork、Hb G－Taipei和Hb G－Coushatta 样本的HbA1c结果偏倚大小不一。结论血红蛋白变异体对不同HbA1c检测系统有着不同的影响，CFP可提示常见血红蛋白变异体。（中华检验医学杂志，2015，38：470－474）
목적：탐토혈홍단백변이체대당화혈홍단백（ HbA1c ）불동검측계통적영향。방법채용매법（Norudia N HbA1c，SEKISUI）、면역투사비탁법（oneHbA1c FS，DiaSys）、친화층석고압액상색보법（Ultra2，Trinity Biotech）、모세관전영법[Capillary 2 Flex Piercing（CFP）Sebia]화리자교환고압액상색보법[HA8160，Arkary；VariantⅡTurbo（Ⅶ－T），Bio－Rad；VariantⅡTurbo 2．0（Ⅶ－T 2．0），Bio－Rad]7충검측계통측정정상양본화혈홍단백변이체양본HbA1c ，분석혈홍단백변이체대HbA1c검측결과적영향。결과대우정상양본，7충검측계통적상관성량호。대우（ HbE）잡합자，이（ CFP）위삼비계통，Ⅶ－T적HbA1c결과분성고저량군，각검측계통적HbA1c결과초과6％비례범위위14．9％～73．1％。대우HbE순합자，Ultra2검측출부분양본적당화혈홍단백결과，CFP화Ⅶ－T 2．0미급출HbA1c결과。상견혈홍단백변이체중，J－Bangkok、Hb J－Bangkok、Hb J－Newyork、Hb G－Taipei화Hb G－Coushatta 양본적HbA1c결과편의대소불일。결론혈홍단백변이체대불동HbA1c검측계통유착불동적영향，CFP가제시상견혈홍단백변이체。（중화검험의학잡지，2015，38：470－474）
Objective To assess influences of hemoglobin variants on different HbA 1c measurement systems.Methods HbA1c values of normal samples and samples with hemoglobin variants were measured respectively byenzymatic assay (Norudia N HbA1c,SEKISUI), immunity transmission turbidity (oneHbA1c FS,DiaSys), boronate affinity HPLC(Ultra2,Trinity Biotech), capillary electrophoresis(Capillary 2 Flex Piercing,Sebia)and ion exchange HPLC(HA8160,Arkary;Variant Ⅱ Turbo(VⅡ-T),Bio-Rad;Variant ⅡTurbo 2.0 (VⅡ-T 2.0), Bio-Rad).HbA1c values from different hemoglobin variants and HbA1c measurement systems were analyzed .Results The HbA1c values from the 7 HbA1c measurement systems were well correlatedin normal samples .For HbE heterozygote , the HbA1c values from VⅡ-T were divided into 2 groups comparing with CFP , and HbA1c differences between CFP and other measurement systems are minor except for HA8160 and VⅡ-T.The HbA1c values of homozygous HbE were given by Ultra 2 but CFP and VⅡ-T 2.0.The differences of HbA1c values from samples with J-Bangkok are much higher than those from the samples with other hemoglobin variants .The differences of HbA1c values from samples with all kinds of hemoglobin variants(Hb J-Bangkok, Hb J-Newyork, Hb G-Taipei and Hb G-Coushatta)are dramatic for VⅡ-T.For rare Kurosaki, CFP can give a hint that there existshemoglobin variant while measuring HbA 1c. Conclusions Capillary Flex 2 Piercingcan well recognize common hemoglobin variants . Different hemoglobin variants have different influences on different HbA 1c measurement systems.The influences of J-Bangkok among HbA1c measurement systems are more evident than the other common hemoglobin variants .