CAJ | 학술논문

目的：研究卷柏多糖对肠道病毒71型（EV71）的抑制作用，并初步探讨其抗病毒机制。方法：测定30%、50%醇沉卷柏多糖对Vero细胞的毒性和对EV71感染致Vero细胞病变的抑制作用，筛选出选择指数（SI）高的卷柏多糖醇沉部位，检测活性高的卷柏多糖部位对EV71的直接灭活作用及对病毒生活周期的影响。结果：30%和50%醇沉卷柏多糖及利巴韦林抑制EV71致细胞病变的IC50值分别为23.62±2.50、10.43±0.38和38.66±4.91 mg/L，SI分别为>169、>384和101，提示50%醇沉卷柏多糖抗EV71活性最好。机制研究显示，50%醇沉卷柏多糖对EV71无直接杀病毒作用；与模型组相比，50%醇沉卷柏多糖可显著抑制EV71对Vero细胞的吸附作用（P<0.05），而对EV71的进入、复制及释放环节无影响。结论：卷柏多糖具有抗EV71活性，可能通过抑制病毒的吸附达到抗病毒作用。
목적：연구권백다당대장도병독71형（EV71）적억제작용，병초보탐토기항병독궤제。방법：측정30%、50%순침권백다당대Vero세포적독성화대EV71감염치Vero세포병변적억제작용，사선출선택지수（SI）고적권백다당순침부위，검측활성고적권백다당부위대EV71적직접멸활작용급대병독생활주기적영향。결과：30%화50%순침권백다당급리파위림억제EV71치세포병변적IC50치분별위23.62±2.50、10.43±0.38화38.66±4.91 mg/L，SI분별위>169、>384화101，제시50%순침권백다당항EV71활성최호。궤제연구현시，50%순침권백다당대EV71무직접살병독작용；여모형조상비，50%순침권백다당가현저억제EV71대Vero세포적흡부작용（P<0.05），이대EV71적진입、복제급석방배절무영향。결론：권백다당구유항EV71활성，가능통과억제병독적흡부체도항병독작용。
Objective: To study the inhibitory activity and mechanism of polysaccharides extracted from Selaginel?la tamariscina using Vero cells infected with enterovirus 71(EV71). Methods: Cytotoxicity and cytopathic effect (CPE) inhibition of 30%, 50% alcohol precipitated polysaccharides from S.tamariscina were measured and the agent with the higher selection index(SI) was determined. Then it was added to Vero cells infected with EV71 to study its direct virucidal activity, and acting phase by time-of-addition assays. Results: IC50 of 30%, 50% alcohol precipitated polysaccharides and ribavirin were 23.62±2.50, 10.43±0.38 and 38.66±4.91 mg/L, respectively, and SI of the agents were respectively >169, >384 and 101. 50% alcohol precipitated polysaccharides showed the best an?ti-EV71 activity. 50% alcohol precipitated polysaccharides had no direct virucidal effect on EV71, but it could in?hibit the attachment of EV71 with Vero cells significantly(P<0.05), but not viral penetration, replication and re?lease phase. Conclusion: 50% alcohol precipitated polysaccharides from S.tamariscina has remarkable anti-EV71 activity by blocking virus attachment.