CAJ | 학술논문

目的：建立高效液相色谱( HPLC)法同时测定复方丹参片和复方丹参滴丸中7种活性成分含量的方法。方法采用Zorbax C18色谱柱,以乙腈-0.1％磷酸溶液为流动相,梯度洗脱,流速1 mL·min-1,检测波长为203,270和281 nm。结果7种成分峰面积与浓度的线性关系良好；加样回收率在95.1％~100.4％；滴丸样品中丹参酮ⅡA未能检出。结论建立了两类复方丹参制剂中同时测定丹参素、原儿茶醛、三七皂苷R1、人参皂苷Rg1、丹酚酸B、人参皂苷Rb1和丹参酮ⅡA含量的方法,精密度高,重复性好,可用于两类复方丹参制剂的质量控制；复方丹参片中丹参酮ⅡA含量明显高于滴丸。
목적：건립고효액상색보( HPLC)법동시측정복방단삼편화복방단삼적환중7충활성성분함량적방법。방법채용Zorbax C18색보주,이을정-0.1％린산용액위류동상,제도세탈,류속1 mL·min-1,검측파장위203,270화281 nm。결과7충성분봉면적여농도적선성관계량호；가양회수솔재95.1％~100.4％；적환양품중단삼동ⅡA미능검출。결론건립료량류복방단삼제제중동시측정단삼소、원인다철、삼칠조감R1、인삼조감Rg1、단분산B、인삼조감Rb1화단삼동ⅡA함량적방법,정밀도고,중복성호,가용우량류복방단삼제제적질량공제；복방단삼편중단삼동ⅡA함량명현고우적환。
Objective To establish a HPLC method for simultaneous determination of seven active components in Fufang Danshen tablets and Fufang Danshen dripping pills. Methods These seven compounds were analyzed simultaneously with a Zorbax C18 column by gradient elution using acetonitrile-0. 1% phosphoric acid solution as mobile phase, the flow rate was 1 mL·min-1 and the detection wavelength was set at 203, 270 and 281 nm, respectively. Results All the seven components showed good linear relation between peak area and concentration of the test, and the average recoveries were between 95. 1%-100. 4%. Tanshinone ⅡA was not detected in samples of dropping pills. Conclusion The HPLC method to determine the components including tanshinone ⅡA, salvianolic acid B, propanoid acid, protocatechuic aldehyde, notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 of the two different Danshen preparations has been established, and it has the advantages of simplicity, high precision, good repeatability, and can be used for the quality control of two kinds of Fufang Danshen preparations. The content of tanshinone Ⅱ A in Fufang Danshen tablet was distinctly higher than that of dropping pills.