CAJ | 학술논문

目的研究胚胎干细胞标志物转录因子(octamer-binding transcription factor4,Oct4)在胃癌细胞中的表达及对细胞增殖和凋亡的影响.方法利用实时荧光定量PCR技术和Western blot方法检测Oct4在不同分化胃癌细胞株中的表达;选择高表达Oct4的胃癌细胞株为对象,构建Oct4siRNA慢病毒载体干扰Oct4在胃癌细胞中的表达,观察Oct4在胃癌细胞中的表达被干扰后对胃癌细胞增殖和凋亡的影响.结果 Oct4在不同胃癌细胞株中均有表达,但在低分化胃癌细胞株MGC-803和中分化胃癌细胞株SGC-7901中表达明显;胃癌细胞SGC-7901在感染siRNA-Oct4慢病毒后相对荧光强度明显降低(P＜0.01),细胞划痕试验结果显示干扰组细胞数显著减少(P＜0.01).Oct4干扰后,细胞凋亡主要发生在G1期,干扰组细胞凋亡率为18.71％,对照组为5.32％,胃癌细胞凋亡比例增加(P＜0.01).结论干扰Oct4的表达可抑制胃癌细胞株的增殖并降低其侵袭能力.
목적 연구배태간세포표지물전록인자(octamer-binding transcription factor4,Oct4)재위암세포중적표체급대세포증식화조망적영향.방법 이용실시형광정량PCR기술화Western blot방법검측Oct4재불동분화위암세포주중적표체;선택고표체Oct4적위암세포주위대상,구건Oct4siRNA만병독재체간우Oct4재위암세포중적표체,관찰Oct4재위암세포중적표체피간우후대위암세포증식화조망적영향.결과 Oct4재불동위암세포주중균유표체,단재저분화위암세포주MGC-803화중분화위암세포주SGC-7901중표체명현;위암세포SGC-7901재감염siRNA-Oct4만병독후상대형광강도명현강저(P＜0.01),세포화흔시험결과현시간우조세포수현저감소(P＜0.01).Oct4간우후,세포조망주요발생재G1기,간우조세포조망솔위18.71％,대조조위5.32％,위암세포조망비례증가(P＜0.01).결론 간우Oct4적표체가억제위암세포주적증식병강저기침습능력.
Objective To investigate the expression of transcription factor Oct 4 in gastric carcinoma and its effects on gastric cancer cell proliferation and apoptosis after Oct 4 gene interfered by lentivirus vector.Methods Real time PCR and Western blot were used to observe the expression of Oct 4 in different differentiated gastric cancer cell lines.Gastric cancer cell lines with high expression of Oct 4 was cultured and infected by siRNA-Oct 4-lentivirus vector.Cell proliferation and apoptosis were observed after Oct 4 gene was interfered.Results Oct 4 was highly expressed in poorly and moderately differentiated gastric cancer cells.Gene interfered with siRNA inhibits the expression of Oct 4 in gastric cancer cells and show significant effects on cell proliferation and mobility as well as apoptosis after down-regulation of Oct 4.Condusions Oct 4 expression is in close relationship with gastric cancer cell proliferation and invasive ability.