CAJ | 학술논문

目的：观察脂联素在SS小鼠颌下腺的表达；通过体内转染重组基因，探讨脂联素在SS小鼠炎症中的作用。方法选取30只非肥胖糖尿病（NOD）小鼠作自发性SS小鼠模型。分别转染脂联素基因重组腺病毒,空病毒载体和生理盐水(对照组),比较3组小鼠颌下腺腺体、病理学改变，TNF-α、脂联素水平。用实时荧光定量（RT）-PCR检测小鼠腺体脂联素表达。结果通过3组小鼠对比发现，对照组小鼠腺体破坏，淋巴细胞浸润，腺泡细胞萎缩变性，rADV-腺病毒组无淋巴细胞浸润；与对照组相比，rADV-腺病毒组TNF-α、颌下腺脂联素mRNA表达[（248±30）和（162±73） ng/ml]均较对照组显著降低（P<0.05）；对照组间差异无统计学意义（P>0.05）。结论局部转染脂联素可明显改善小鼠腺体形态学、降低TNF-α水平，抑制小鼠涎腺炎症反应，降低脂联素表达，脂联素可能为SS小鼠保护基因。
목적：관찰지련소재SS소서합하선적표체；통과체내전염중조기인，탐토지련소재SS소서염증중적작용。방법선취30지비비반당뇨병（NOD）소서작자발성SS소서모형。분별전염지련소기인중조선병독,공병독재체화생리염수(대조조),비교3조소서합하선선체、병이학개변，TNF-α、지련소수평。용실시형광정량（RT）-PCR검측소서선체지련소표체。결과통과3조소서대비발현，대조조소서선체파배，림파세포침윤，선포세포위축변성，rADV-선병독조무림파세포침윤；여대조조상비，rADV-선병독조TNF-α、합하선지련소mRNA표체[（248±30）화（162±73） ng/ml]균교대조조현저강저（P<0.05）；대조조간차이무통계학의의（P>0.05）。결론국부전염지련소가명현개선소서선체형태학、강저TNF-α수평，억제소서연선염증반응，강저지련소표체，지련소가능위SS소서보호기인。
Objective To observe the expression of AdipoQ in Sj?gren's syndrome (SS) mice and its role in inflammation was investigated by recombinant gene transfection study in vivo. Methods As spon-taneous SS mice model, a total of 30 NOD mice were divided into 3 groups randomly: recombinant adenovirus (rADV-AdipoQ) group, normal saline control and simple adenoviruses (control group). The submandibular gland morphology, histopathological grading and the level of serum tumor necrosis factor-α (TNF-α), AdipoQ was compared between the three groups. The expression of AdipoQ on mice submandibular gland was assessed by means of semi-quantitative reverse transcriptase polymerase chain reaction. Results The submandibular glands of the mice of the control group were destructed, with focal lymphocytic infiltration and acinus atrophy. Compared with control model groups, the serum TNF-α and salivary gland AdipoQ expression was significantly down-regulated in the rADV-AdipoQ group [(248 ±30) vs (162 ±73) ng/ml] (P<0.05). Conclusion AdipoQ gene transfected SS mice can significantly improve the morphological features of tissues and decrease the concentration of TNF-αin serum, in addition, it can effectively inhibit inflammation, decrease the degree of protein and AdipoQgene expression. So the AdipoQ may be the protective gene in SS mice.