山东农业大学学报（自然科学版）
山東農業大學學報（自然科學版）
산동농업대학학보（자연과학판）
JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2015年
4期
540-543
,共4页
P2X2受体%神经节内板状末梢%降钙素基因相关肽%食管%小鼠
P2X2受體%神經節內闆狀末梢%降鈣素基因相關肽%食管%小鼠
P2X2수체%신경절내판상말소%강개소기인상관태%식관%소서
P2X2 receptor%intraganglionic laminar endings%calcitonin gene-related peptide%esophagus%mouse
本文采用免疫荧光组织化学双标方法结合激光共聚焦显微镜技术研究了伤害性受体P2X2阳性感觉纤维末梢在小鼠食管内的分布及与降钙素基因相关肽（CGRP）阳性纤维的关系。结果表明：在食管各水平均可见到P2X2阳性感觉纤维末梢。P2X2阳性纤维集中分布于食管的内、外肌层之间，部分或全部的覆盖在肌间神经节的表面。一些串珠样的纤维深入到肌间神经节内，相互缠绕在一起形成许多结构复杂、形状各异的神经节内板状末稍（IGLEs）。荧光双标显示许多CGRP阳性纤维围绕在P2X2阳性IGLEs周围，没有见到P2X2和CGRP在同一纤维的共存。结状神经节内可见许多中小型神经元表达P2X2，P2X2/CGRP双标神经元稀少，而背根神经节内可见较多的P2X2阳性细胞含有CGRP。由此可见，小鼠食管内部分感觉神经末梢表达伤害性受体P2X2，并形成IGLEs样末梢结构。
本文採用免疫熒光組織化學雙標方法結閤激光共聚焦顯微鏡技術研究瞭傷害性受體P2X2暘性感覺纖維末梢在小鼠食管內的分佈及與降鈣素基因相關肽（CGRP）暘性纖維的關繫。結果錶明：在食管各水平均可見到P2X2暘性感覺纖維末梢。P2X2暘性纖維集中分佈于食管的內、外肌層之間，部分或全部的覆蓋在肌間神經節的錶麵。一些串珠樣的纖維深入到肌間神經節內，相互纏繞在一起形成許多結構複雜、形狀各異的神經節內闆狀末稍（IGLEs）。熒光雙標顯示許多CGRP暘性纖維圍繞在P2X2暘性IGLEs週圍，沒有見到P2X2和CGRP在同一纖維的共存。結狀神經節內可見許多中小型神經元錶達P2X2，P2X2/CGRP雙標神經元稀少，而揹根神經節內可見較多的P2X2暘性細胞含有CGRP。由此可見，小鼠食管內部分感覺神經末梢錶達傷害性受體P2X2，併形成IGLEs樣末梢結構。
본문채용면역형광조직화학쌍표방법결합격광공취초현미경기술연구료상해성수체P2X2양성감각섬유말소재소서식관내적분포급여강개소기인상관태（CGRP）양성섬유적관계。결과표명：재식관각수평균가견도P2X2양성감각섬유말소。P2X2양성섬유집중분포우식관적내、외기층지간，부분혹전부적복개재기간신경절적표면。일사천주양적섬유심입도기간신경절내，상호전요재일기형성허다결구복잡、형상각이적신경절내판상말초（IGLEs）。형광쌍표현시허다CGRP양성섬유위요재P2X2양성IGLEs주위，몰유견도P2X2화CGRP재동일섬유적공존。결상신경절내가견허다중소형신경원표체P2X2，P2X2/CGRP쌍표신경원희소，이배근신경절내가견교다적P2X2양성세포함유CGRP。유차가견，소서식관내부분감각신경말소표체상해성수체P2X2，병형성IGLEs양말소결구。
The distribution of P2X2 immunoreactive afferent endings and the relationship with calcitonin gene-related peptide (CGRP) immunoreactive fibers in the mouse esophagus was studied using double immunofluorescence method combined confocal laser scanning microscope technique. P2X2 immunoreactive endings were seen in sections from all levels of the mouse esophagus. The distribution of P2X2 immunoreactive structures concentrated in the area between outer and inner muscular layers, covered myenteric ganglia totally or partly. The dense clusters of fine varicose P2X2 immunoreactive terminals protruded into myenteric ganglia forming complexes of profuse laminar structures for intraganglionic laminar endings (IGLEs). Double immunofluorescence for P2X2 and CGRP revealed that numerous varicose fibers immunoreactive for CGRP closely surrounded P2X2 immunopositive IGLEs. However, P2X2 immunoreactive terminals never expressed CGRP immunoreactivity. In nodose ganglion, numerous neuronal cell bodies in small- and middle-sized displayed P2X2 immunoreactivity. P2X2/CGRP double-labeling neurons were rare. However, many P2X2 positive neurons in dorsal root ganglion contained CGRP immunoreactivity. These results demonstrated that some afferent endings in mouse esophagus expressed nociceptor P2X2, and formed IGLEs structures.
