广州医科大学学报
廣州醫科大學學報
엄주의과대학학보
Academic Journal of Guangzhou Medical College
2015年
3期
32-36
,共5页
李强%郭壮波%伍光颖%李超霞%钟志勇
李彊%郭壯波%伍光穎%李超霞%鐘誌勇
리강%곽장파%오광영%리초하%종지용
内皮微颗粒%急性心肌缺血%心肌细胞凋亡
內皮微顆粒%急性心肌缺血%心肌細胞凋亡
내피미과립%급성심기결혈%심기세포조망
endothelial microparticle%acute myocardial ischemia%myocardial apoptosis
目的：探讨内皮微颗粒作为早期急性心肌缺血评估指标的可能性。方法：将24只雄性 SD 大鼠随机分为假手术组（Sham 组）8只、心肌梗死组（MI 组）8只和心肌缺血再灌注组（I/ R 组）8只，采用冠状动脉结扎制作急性心肌缺血大鼠模型。分别在造模后2 h 和24 h 采外周血检测肌酸激酶同工酶（creatine kinase-MB，CK-MB ），肌钙蛋白 T（ cardiac troponin T，cTNT ），内皮微颗粒（ endothelial mIcroparticles，EMP），其中循环 EMP 用流式细胞仪检测。通过末端标记法（ terminal-deoxynucleoitidyl transferase mediated nick end labeling，TUNEL）检测心肌细胞凋亡情况。结果：造模后2 h 与24 h，I/ R 组大鼠 EMP 水平均显著低于 MI 组（P﹤0.05），造模后2 h MI 组、I/ R 组大鼠的心肌细胞凋亡率均明显高于Sham 组（P﹤0.05）。各组间比较，I/ R 组大鼠的 cTNT 表达水平造模后24 h 较造模后2 h 显著下降，差异有统计学意义（P﹤0.05）；MI 组大鼠的 CK-MB 表达水平造模后24 h 较造模后2 h 显著性升高（P﹤0.05）；而 EMP 与心肌细胞凋亡率各组间比较差异无统计学意义。结论：内皮微颗粒可以作为早期急性心肌缺血的评估指标。
目的：探討內皮微顆粒作為早期急性心肌缺血評估指標的可能性。方法：將24隻雄性 SD 大鼠隨機分為假手術組（Sham 組）8隻、心肌梗死組（MI 組）8隻和心肌缺血再灌註組（I/ R 組）8隻，採用冠狀動脈結扎製作急性心肌缺血大鼠模型。分彆在造模後2 h 和24 h 採外週血檢測肌痠激酶同工酶（creatine kinase-MB，CK-MB ），肌鈣蛋白 T（ cardiac troponin T，cTNT ），內皮微顆粒（ endothelial mIcroparticles，EMP），其中循環 EMP 用流式細胞儀檢測。通過末耑標記法（ terminal-deoxynucleoitidyl transferase mediated nick end labeling，TUNEL）檢測心肌細胞凋亡情況。結果：造模後2 h 與24 h，I/ R 組大鼠 EMP 水平均顯著低于 MI 組（P﹤0.05），造模後2 h MI 組、I/ R 組大鼠的心肌細胞凋亡率均明顯高于Sham 組（P﹤0.05）。各組間比較，I/ R 組大鼠的 cTNT 錶達水平造模後24 h 較造模後2 h 顯著下降，差異有統計學意義（P﹤0.05）；MI 組大鼠的 CK-MB 錶達水平造模後24 h 較造模後2 h 顯著性升高（P﹤0.05）；而 EMP 與心肌細胞凋亡率各組間比較差異無統計學意義。結論：內皮微顆粒可以作為早期急性心肌缺血的評估指標。
목적：탐토내피미과립작위조기급성심기결혈평고지표적가능성。방법：장24지웅성 SD 대서수궤분위가수술조（Sham 조）8지、심기경사조（MI 조）8지화심기결혈재관주조（I/ R 조）8지，채용관상동맥결찰제작급성심기결혈대서모형。분별재조모후2 h 화24 h 채외주혈검측기산격매동공매（creatine kinase-MB，CK-MB ），기개단백 T（ cardiac troponin T，cTNT ），내피미과립（ endothelial mIcroparticles，EMP），기중순배 EMP 용류식세포의검측。통과말단표기법（ terminal-deoxynucleoitidyl transferase mediated nick end labeling，TUNEL）검측심기세포조망정황。결과：조모후2 h 여24 h，I/ R 조대서 EMP 수평균현저저우 MI 조（P﹤0.05），조모후2 h MI 조、I/ R 조대서적심기세포조망솔균명현고우Sham 조（P﹤0.05）。각조간비교，I/ R 조대서적 cTNT 표체수평조모후24 h 교조모후2 h 현저하강，차이유통계학의의（P﹤0.05）；MI 조대서적 CK-MB 표체수평조모후24 h 교조모후2 h 현저성승고（P﹤0.05）；이 EMP 여심기세포조망솔각조간비교차이무통계학의의。결론：내피미과립가이작위조기급성심기결혈적평고지표。
Objective:To investigate the possibility of endothelial microparticles( EMP)being used as evaluation index for early acute myocardial ischemia.Methods:Twenty-four male SD rats were randomly divided into sham operation group,myocardial infarction( MI)group,and ischemia reperfusion( I/ R)group( n = 8 each). The rat models with acute myocardial ischemia were prepared by coronary artery ligation. At 2h and 24h after modeling,peripheral blood was taken to measure creatine kinase-MB( CK-MB),cardiac troponin T (cTNT),and EMP. The circulating level of EMP were measured by flow cytometry. The myocardial apoptosis was determined by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)assay.Results:At 2h and 24 h after modeling,the EMP level in I/ R group was significantly lower than that in MI group(P﹤0.05). At 2 h after modeling,the myocardial apoptosis in MI group and I/ R group was significantly higher than that in Sham operation group(P﹤ 0. 05). The expression level of cTNT in I/ R group significantly decreased at 24 h after modeling compared with that at 2h after modeling,with statistical difference(P﹤0.05). The expression level of CK-MB in MI group significantly increased at 24h after modeling compared with that at 2 h after modeling(P﹤0. 05). There were no statistical differences in EMP and cardiomyocyte apoptosis between different groups. Conclusion:EMP can be used as evaluation index for early acute myocardial ischemia.
