CAJ | 학술논문

本试验运用454焦磷酸高通量测序的方法对施用多抗菌剂的植烟土壤进行细菌群落结构分析。结果发现：植烟土壤中细菌的种类主要集中在变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、芽单胞菌门(Gemmatimonadetes)和厚壁菌门(Firmicutes)。施用多抗菌剂与未施用多抗菌剂的样品相比，根际土壤中细菌总纲数减少7.27%，检测到的OTU数目减少12.83%；非根际土中，细菌的总纲数减少4.62%，检测到的OTU数目减少20.31%。可见，施用多抗菌剂可以降低植烟土壤中细菌群落多样性。
본시험운용454초린산고통량측서적방법대시용다항균제적식연토양진행세균군락결구분석。결과발현：식연토양중세균적충류주요집중재변형균문(Proteobacteria)、방선균문(Actinobacteria)、산간균문(Acidobacteria)、아단포균문(Gemmatimonadetes)화후벽균문(Firmicutes)。시용다항균제여미시용다항균제적양품상비，근제토양중세균총강수감소7.27%，검측도적OTU수목감소12.83%；비근제토중，세균적총강수감소4.62%，검측도적OTU수목감소20.31%。가견，시용다항균제가이강저식연토양중세균군락다양성。
We used 454 pyrophosphate sequencing approach to analyze the bacterial com-munity structure of the tobacco planting soils with multiple antimicrobial inoculant. The results showed that the bacterium in tobacco planting soil which was detected in this experiment belong to five phyla. They are Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Firmicutes compared with rizosphere soil without using multiple antimicrobial inoculants, the number of class which belonged to rhizosphere soil with multiple antimicrobial inoculant reduced 7.27%, and the number of OTU reduced 12.83%. Compared with non-rizosphere soil without using multiple antimicrobial inoculants, the number of class which belonged to non-rhizosphere soil with multiple antimicrobial inoculant reduced 4.62%, and the number of OTU reduced 20.31%. Obviously, multiple antimicrobial inoculantis can lead the decrease in microbial diversity of tobacco planting soil.