CAJ | 학술논문

目的探讨血清素对成骨细胞Ⅰ型胶原蛋白合成的影响。方法用不同浓度（50、100、200和400 ng／L）的血清素处理人成骨细胞，即时定量PCR（ RT-PCR）观察成骨细胞中Ⅰ型胶原蛋白和赖氨酰氧化酶（ LOX）含量的变化。用LOX siRNA干扰LOX蛋白的表达后，用Western blot法检测干扰效果，用酶联免疫吸附实验检测Ⅰ型胶原蛋白含量的变化。用RT-PCR检测血清素处理成骨细胞后Smad2蛋白、Smad3蛋白表达量的变化和Smad3基因敲除对LOX蛋白表达的影响。结果血清素上调成骨细胞中Ⅰ型胶原蛋白和LOX的表达水平，LOX siRNA可显著抑制Ⅰ型胶原蛋白的表达。此外，血清素上调了成骨细胞中Smad2蛋白和Smad3蛋白的表达，干扰Smad3基因的转录，抑制LOX蛋白的表达。结论血清素通过Smad信号通路上调LOX的表达，并促进成骨细胞Ⅰ型胶原蛋白的合成。
목적탐토혈청소대성골세포Ⅰ형효원단백합성적영향。방법용불동농도（50、100、200화400 ng／L）적혈청소처리인성골세포，즉시정량PCR（ RT-PCR）관찰성골세포중Ⅰ형효원단백화뢰안선양화매（ LOX）함량적변화。용LOX siRNA간우LOX단백적표체후，용Western blot법검측간우효과，용매련면역흡부실험검측Ⅰ형효원단백함량적변화。용RT-PCR검측혈청소처리성골세포후Smad2단백、Smad3단백표체량적변화화Smad3기인고제대LOX단백표체적영향。결과혈청소상조성골세포중Ⅰ형효원단백화LOX적표체수평，LOX siRNA가현저억제Ⅰ형효원단백적표체。차외，혈청소상조료성골세포중Smad2단백화Smad3단백적표체，간우Smad3기인적전록，억제LOX단백적표체。결론혈청소통과Smad신호통로상조LOX적표체，병촉진성골세포Ⅰ형효원단백적합성。
Objective To explore the mechanism of serotonin-promoted osteoblast differentiation.Methods Expression levels of collagen Ⅰand lysyl oxidase ( LOX) in osteoblast were measured by RT-PCR after treated by (50, 100, 200 and 400 ng/L) serotonin.LOX siRNA effect was measured by Western blot, and protein levels of collagen Ⅰ were determined by ELISA after treated by serotonin.Expression levels of Smad2 and Smad3 in osteoblasts were also measured by RT-PCR after treated by serotonin.Moreover , expression levels of LOX were measured by RT-PCR after Smad3 was knockout.Results Serotonin promoted collagenⅠ and LOX expression.The expression level of collagenⅠ was significantly decreased by LOX siRNA.Furthermore , serotonin up-regulated the expression of Smad 2 and Smad3 in osteoblasts , and the expression level of LOX was inhibited by Smad 3 siRNA.Conclusion Serotonin promoted collagenⅠexpression by activating Smads signaling pathway and up-regulating the LOX expression.