检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2015年
15期
2188-2189,2192
,共3页
胶体金法%全自动生化分析仪%肌酸激酶同工酶
膠體金法%全自動生化分析儀%肌痠激酶同工酶
효체금법%전자동생화분석의%기산격매동공매
colloidal gold method%fully automatic biochemical analyzer%CK-MB
目的：探讨胶体金法与全自动生化分析仪检测肌酸激酶同工酶（CK‐MB）的相关性及差异，为临床应用提供依据。方法选取2013年1月至2014年12月重庆市精神卫生中心歌乐山院区收治的心肌损伤患者血清标本327例，采用胶体金法及全自动生化分析仪检测同一血清标本 CK‐MB 浓度及活性，比较2种方法测定结果的相关性及差异。结果全自动生化分析仪测出的 CK‐MB 活性范围为30～110 U ／L 的标本数量与胶体金法测定CK‐MB 显示“＋”（浓度范围5．0～20．0 ng／mL ）的标本数量符合率为95．97％；全自动生化分析仪测出的 CK‐MB活性范围为110～450 U ／L 的标本数量与胶体金法测定 CK‐MB 显示“＋＋”（浓度范围20．0～100．0 ng／mL）的标本数量符合率为95．41％；全自动生化分析仪测出的 CK‐MB 活性范围大于450 U ／L 的标本数量与胶体金法测定的CK‐MB 显示“＋＋＋”（浓度范围大于100 ng／mL）的标本数量符合率为94．68％。结论胶体金法测定的 CK‐MB的显色强度提示的浓度范围与全自动生化分析仪测定的血清 CK‐MB 活性范围呈一定比例的对应关系。
目的：探討膠體金法與全自動生化分析儀檢測肌痠激酶同工酶（CK‐MB）的相關性及差異，為臨床應用提供依據。方法選取2013年1月至2014年12月重慶市精神衛生中心歌樂山院區收治的心肌損傷患者血清標本327例，採用膠體金法及全自動生化分析儀檢測同一血清標本 CK‐MB 濃度及活性，比較2種方法測定結果的相關性及差異。結果全自動生化分析儀測齣的 CK‐MB 活性範圍為30～110 U ／L 的標本數量與膠體金法測定CK‐MB 顯示“＋”（濃度範圍5．0～20．0 ng／mL ）的標本數量符閤率為95．97％；全自動生化分析儀測齣的 CK‐MB活性範圍為110～450 U ／L 的標本數量與膠體金法測定 CK‐MB 顯示“＋＋”（濃度範圍20．0～100．0 ng／mL）的標本數量符閤率為95．41％；全自動生化分析儀測齣的 CK‐MB 活性範圍大于450 U ／L 的標本數量與膠體金法測定的CK‐MB 顯示“＋＋＋”（濃度範圍大于100 ng／mL）的標本數量符閤率為94．68％。結論膠體金法測定的 CK‐MB的顯色彊度提示的濃度範圍與全自動生化分析儀測定的血清 CK‐MB 活性範圍呈一定比例的對應關繫。
목적：탐토효체금법여전자동생화분석의검측기산격매동공매（CK‐MB）적상관성급차이，위림상응용제공의거。방법선취2013년1월지2014년12월중경시정신위생중심가악산원구수치적심기손상환자혈청표본327례，채용효체금법급전자동생화분석의검측동일혈청표본 CK‐MB 농도급활성，비교2충방법측정결과적상관성급차이。결과전자동생화분석의측출적 CK‐MB 활성범위위30～110 U ／L 적표본수량여효체금법측정CK‐MB 현시“＋”（농도범위5．0～20．0 ng／mL ）적표본수량부합솔위95．97％；전자동생화분석의측출적 CK‐MB활성범위위110～450 U ／L 적표본수량여효체금법측정 CK‐MB 현시“＋＋”（농도범위20．0～100．0 ng／mL）적표본수량부합솔위95．41％；전자동생화분석의측출적 CK‐MB 활성범위대우450 U ／L 적표본수량여효체금법측정적CK‐MB 현시“＋＋＋”（농도범위대우100 ng／mL）적표본수량부합솔위94．68％。결론효체금법측정적 CK‐MB적현색강도제시적농도범위여전자동생화분석의측정적혈청 CK‐MB 활성범위정일정비례적대응관계。
Objective To explore the correlation and difference between the colloidal gold method and the fully automatic biochemical analyzer for detecting the creatine kinase isoenzyme(CK‐MB) .Methods 327 serum specimens from the patients with myocardial injury in our hospital from January 2013 to December 2014 were analyzed .The col‐loidal gold method and the fully automatic biochemical analyzer were adopted to measure the CK‐MB concentration of the same sample .The corresponding relation and difference of the detection results were compared between the two methods .Results The coincidence rate of the sample number for the CK‐MB activity range of 30 - 110 U /L detected by the fully automatic biochemical analyzer and which for the CK‐MB concentration range of 5 .0 - 20 .0 ng/mL dis‐playing as “ + ” detected by the colloidal gold method was 95 .97% ;the coincidence rate of the sample number for the CK‐MB activity range of 110 - 450 U /L detected by the fully automatic biochemical analyzer and which for the CK‐MB concentration range of 20 .0 - 100 .0 ng/mL displaying “ + + ” detected by the colloidal gold method was 95 .41% ;the coincidence rate of sample number for the CK‐MB activity range over 450 U /L and which for the CK‐MB concentration range over 100 ng/mL displaying “ + + + ” detected by the colloidal gold method was 94 .68% . Conclusion The concentration range prompted by the serum CK‐MB color intensity detected by the colloidal gold method has certain proportional corresponding relation with the CK‐MB activity range detected by the fully automatic biochemical analyzer .
