中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2015年
14期
2722-2725
,共4页
葡萄糖%细胞凋亡%视网膜%Müller 细胞%重组人促红细胞生成素
葡萄糖%細胞凋亡%視網膜%Müller 細胞%重組人促紅細胞生成素
포도당%세포조망%시망막%Müller 세포%중조인촉홍세포생성소
Glucose%Apoptosis%Retina%Müller cell%Recombinant human erythropoietin
目的：探讨促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞的保护作用。方法体外传代培养大鼠视网膜Müller细胞，分组为N组（正常对照），G组（25 mmol/L葡萄糖）， E1G组（1×104 IU/L rhEPO＋25 mmol/L葡萄糖），E2G组（2×104 IU/L rhEPO＋25 mmol/L葡萄糖）， E4G组（4×104 IU/L rhEPO＋25 mmol/L葡萄糖），MTT比色法比较五组视网膜Müller细胞活力的改变。酶联免疫吸附试验检测各组细胞caspase-3蛋白表达的情况。结果 G组、E1G组、E2G组、E4G组细胞活力均低于N组，E1G组、E2G组、E4G组细胞活力高于G组，E4G组高于E2G组，E2G组高于E1G组。酶联免疫吸附试验检测结果显示G组caspase-3表达高于N组，rhEPO干预各组caspase-3表达均低于G组，并随EPO干预浓度增高表达降低，但均高于N组。结论促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞有保护作用。
目的：探討促紅細胞生成素對高濃度葡萄糖培養大鼠視網膜Müller細胞的保護作用。方法體外傳代培養大鼠視網膜Müller細胞，分組為N組（正常對照），G組（25 mmol/L葡萄糖）， E1G組（1×104 IU/L rhEPO＋25 mmol/L葡萄糖），E2G組（2×104 IU/L rhEPO＋25 mmol/L葡萄糖）， E4G組（4×104 IU/L rhEPO＋25 mmol/L葡萄糖），MTT比色法比較五組視網膜Müller細胞活力的改變。酶聯免疫吸附試驗檢測各組細胞caspase-3蛋白錶達的情況。結果 G組、E1G組、E2G組、E4G組細胞活力均低于N組，E1G組、E2G組、E4G組細胞活力高于G組，E4G組高于E2G組，E2G組高于E1G組。酶聯免疫吸附試驗檢測結果顯示G組caspase-3錶達高于N組，rhEPO榦預各組caspase-3錶達均低于G組，併隨EPO榦預濃度增高錶達降低，但均高于N組。結論促紅細胞生成素對高濃度葡萄糖培養大鼠視網膜Müller細胞有保護作用。
목적：탐토촉홍세포생성소대고농도포도당배양대서시망막Müller세포적보호작용。방법체외전대배양대서시망막Müller세포，분조위N조（정상대조），G조（25 mmol/L포도당）， E1G조（1×104 IU/L rhEPO＋25 mmol/L포도당），E2G조（2×104 IU/L rhEPO＋25 mmol/L포도당）， E4G조（4×104 IU/L rhEPO＋25 mmol/L포도당），MTT비색법비교오조시망막Müller세포활력적개변。매련면역흡부시험검측각조세포caspase-3단백표체적정황。결과 G조、E1G조、E2G조、E4G조세포활력균저우N조，E1G조、E2G조、E4G조세포활력고우G조，E4G조고우E2G조，E2G조고우E1G조。매련면역흡부시험검측결과현시G조caspase-3표체고우N조，rhEPO간예각조caspase-3표체균저우G조，병수EPO간예농도증고표체강저，단균고우N조。결론촉홍세포생성소대고농도포도당배양대서시망막Müller세포유보호작용。
Objective To investigate the protective effect of erythropoietin on cultured retinal Müller cells injured by high glucose. Methods Neonatal rats’ retinal Müller cells of serial subcultivation were divided into N (normal control group), G (25 mmol/L glucose) group, E1G (1×104 IU/L recombinant human erythropoietin+25 mmol/L glucose) group, E2G (2×104 IU/L recombinant human erythropoietin+25 mmol/L glucose) group and E4G (4×104 IU/L recombinant human erythropoietin+25 mmol/L glucose) group. MTT assay was applied to detect cells viability in five groups. The expression of caspase-3 was detected with enzyme linked immunosorbent assay. Results Cell viability of G group, E1G group, E2G group and E4C group were lower than that of N group. Cell viability of E1G group, E2G group and E4C group were higher than that of G group, E4G group was higher than that of E2G group and E2G group was higher than that of E1G group. The expression of caspase-3 in E1G group, E2G group and E4G group were lower than that in G group which was high compared with N group. The expression of caspase-3 in E1G group, E2G group and E4G group were lower than that in G group which was high compared with N group. With the increase of rhEPO, the expression of caspase-3 was reducing. Conclusion Erythropoietin pretreatment could effectively protect the cultured retinal Müller cells from injuries induced by high glucose.
