CAJ | 학술논문

通过温室中进行的微宇宙实验，探讨了不同浓度邻苯二甲酸二甲酯（Dimethyl phthalate, DMP）污染对黑土土壤呼吸和酶学活性的影响。研究结果表明，DMP污染处理后黑土微生物呼吸速率和代谢熵均表现为低浓度（5 mg·kg-1）促进，高浓度（10、20、40 mg·kg-1）抑制，且抑制效应与DMP污染浓度正相关；DMP污染对黑土过氧化氢酶具有抑制效应，对黑土转化酶和脲酶呈现为激活效应，且与污染浓度正相关；DMP污染对黑土多酚氧化酶和蛋白酶呈现随时间波动而变化的趋势，多酚氧化酶表现为先抑制后促进，蛋白酶则为先促进后抑制。通过典型对应性分析发现DMP是影响黑土酶学性质改变的主要因素，DMP污染改变了黑土代谢特征，进而可能影响黑土的生态系统功能。
통과온실중진행적미우주실험，탐토료불동농도린분이갑산이갑지（Dimethyl phthalate, DMP）오염대흑토토양호흡화매학활성적영향。연구결과표명，DMP오염처리후흑토미생물호흡속솔화대사적균표현위저농도（5 mg·kg-1）촉진，고농도（10、20、40 mg·kg-1）억제，차억제효응여DMP오염농도정상관；DMP오염대흑토과양화경매구유억제효응，대흑토전화매화뇨매정현위격활효응，차여오염농도정상관；DMP오염대흑토다분양화매화단백매정현수시간파동이변화적추세，다분양화매표현위선억제후촉진，단백매칙위선촉진후억제。통과전형대응성분석발현DMP시영향흑토매학성질개변적주요인소，DMP오염개변료흑토대사특정，진이가능영향흑토적생태계통공능。
Dimethyl phthalate(DMP), one of the phthalate esters, is ubiquitous environmental pollutant in soil, and is listed as an environ-mental priority pollutant by China's Ministry of Environmental Protection(MEP). Black soil(Mollisols)is extremely important soil resources and mainly distributes in Northeast China. In this study, a microcosm model was set up to estimate the impacts of DMP contamination on mi-crobial respiration and enzyme activities in black soil. Microbial respiratory rates and metabolic quotients of black soil were promoted by low DMP concentration(5 mg·kg-1), but inhibited by high DMP concentrations(10 mg·kg-1, 20 mg·kg-1 and 40 mg·kg-1). The inhibition was positively correlated with DMP concentrations. DMP inhibited catalase, but promoted invertase and urease in dose-effect manner. Activities of polyphenol oxidase and protease fluctuated during incubation course. Polyphenol oxidase activity showed initial inhibition followed by promotion;whereas protease activity displayed initial promotion followed by inhibition. Canonical correspondence analysis showed that DMP could be the key factor that alters the metabolic characteristics of black soil and impacts ecosystem functions by affecting the enzymatic properties of black soil.