广西植物
廣西植物
엄서식물
GUIHAIA
2015年
4期
604-609
,共6页
香樟果皮%花色苷%提取%响应面法%抗氧化
香樟果皮%花色苷%提取%響應麵法%抗氧化
향장과피%화색감%제취%향응면법%항양화
camphor pericarp%anthocyanins%extraction%response surface method%antioxidant
为了优化香樟果皮花色苷的最佳提取工艺条件,用乙醇作为提取溶剂,选取提取时间、提取温度、提取剂浓度、料液比、pH 五因素,采用 Box-Behnken 建立三因素三水平模型,用响应面法优化各因素及其相互作用的最佳组合,用 Design-Expert 8．0设计回归正交实验；同时用水杨酸比色法和 DPPH 法,测定了香樟果皮花色苷的抗氧化能力,并比较了不同放置时间对香樟果花色苷稳定性的影响.结果表明：香樟果花色苷最佳提取工艺的回归方程为Y ＝58．64＋2．27A ＋12．78B ＋10．18C －14．01A 2－11．00B 2－7．56C 2,R 2＝0．9796,模型拟合程度良好,在该试验范围内,模型能够准确反映花色苷的提取结果；最佳工艺参数分别为 pH1．0、料液比1∶15、乙醇浓度78．59％、提取温度77．14℃、提取时间42．48 min,在此条件下香樟果花色苷的得率为67．99 mg·100 g-1；在一定浓度范围内,香樟果皮花色苷清除羟自由基率和总抗氧化能力均与浓度成正线性相关,回归方程分别为 y ＝0．3388x ＋13．485(R 2＝0．9856),y ＝0．0275x ＋0．0221,(R 2＝0．9966).利用响应面法确定的最佳工艺条件合理,可用于香樟果皮花色苷的提取,同时香樟果花色苷具有良好的抗氧化活性,为天然色素的开发利用提供一定指导意义.
為瞭優化香樟果皮花色苷的最佳提取工藝條件,用乙醇作為提取溶劑,選取提取時間、提取溫度、提取劑濃度、料液比、pH 五因素,採用 Box-Behnken 建立三因素三水平模型,用響應麵法優化各因素及其相互作用的最佳組閤,用 Design-Expert 8．0設計迴歸正交實驗；同時用水楊痠比色法和 DPPH 法,測定瞭香樟果皮花色苷的抗氧化能力,併比較瞭不同放置時間對香樟果花色苷穩定性的影響.結果錶明：香樟果花色苷最佳提取工藝的迴歸方程為Y ＝58．64＋2．27A ＋12．78B ＋10．18C －14．01A 2－11．00B 2－7．56C 2,R 2＝0．9796,模型擬閤程度良好,在該試驗範圍內,模型能夠準確反映花色苷的提取結果；最佳工藝參數分彆為 pH1．0、料液比1∶15、乙醇濃度78．59％、提取溫度77．14℃、提取時間42．48 min,在此條件下香樟果花色苷的得率為67．99 mg·100 g-1；在一定濃度範圍內,香樟果皮花色苷清除羥自由基率和總抗氧化能力均與濃度成正線性相關,迴歸方程分彆為 y ＝0．3388x ＋13．485(R 2＝0．9856),y ＝0．0275x ＋0．0221,(R 2＝0．9966).利用響應麵法確定的最佳工藝條件閤理,可用于香樟果皮花色苷的提取,同時香樟果花色苷具有良好的抗氧化活性,為天然色素的開髮利用提供一定指導意義.
위료우화향장과피화색감적최가제취공예조건,용을순작위제취용제,선취제취시간、제취온도、제취제농도、료액비、pH 오인소,채용 Box-Behnken 건립삼인소삼수평모형,용향응면법우화각인소급기상호작용적최가조합,용 Design-Expert 8．0설계회귀정교실험；동시용수양산비색법화 DPPH 법,측정료향장과피화색감적항양화능력,병비교료불동방치시간대향장과화색감은정성적영향.결과표명：향장과화색감최가제취공예적회귀방정위Y ＝58．64＋2．27A ＋12．78B ＋10．18C －14．01A 2－11．00B 2－7．56C 2,R 2＝0．9796,모형의합정도량호,재해시험범위내,모형능구준학반영화색감적제취결과；최가공예삼수분별위 pH1．0、료액비1∶15、을순농도78．59％、제취온도77．14℃、제취시간42．48 min,재차조건하향장과화색감적득솔위67．99 mg·100 g-1；재일정농도범위내,향장과피화색감청제간자유기솔화총항양화능력균여농도성정선성상관,회귀방정분별위 y ＝0．3388x ＋13．485(R 2＝0．9856),y ＝0．0275x ＋0．0221,(R 2＝0．9966).이용향응면법학정적최가공예조건합리,가용우향장과피화색감적제취,동시향장과화색감구유량호적항양화활성,위천연색소적개발이용제공일정지도의의.
The extraction technology of camphor pericarp anthocyanins was optimized preferably.The alcohol was used as the extracting solvent.Extracting time and temperature,solvent concentrations,proportion of material and solution and pH were discussed.The model of three factors and three levels was established through box-Behnken.The design of experiment was made by Design-Expert 8.0 software and optimization of various factors and their interaction effects were analyzed by response surface method.The ability of antioxidant was determined with colorimetric method using salicylic acid and DPPH method,and comparison of time to influence the stability of the anthocyanins.The regression equation of the best extraction of anthocyanins was Y = 58.64 + 2.27A + 12.78B +10.18C -14.01A 2 -11.00B 2 -7.56C 2 ,R 2 = 0.9796,and the fitting degree of model was good.Within the range of the test,the model reflected the extraction results.The results showed that the best extraction parameters were pH 1.0,material-solvent ratio 1 g∶15 mL,ethanol content 78.59%,extraction temperature 77.14 ℃,extraction time 42.48 min.On this extraction condition,the content of the camphor pericarp anthocyanins was 67.99 mg·100 g-1 .In a certain concentration,the ability of scavenging hydroxyl free radical and the total antioxidant capability were allpositive linear correlation with the concentration of anthocyanins.The regression equation respectively were y =0.3388x +13.485(R 2 =0.9856),y =0.0275x +0.0221,(R 2 =0.9966).To determine the optimum technological con-ditions by using the response surface method were reasonable,it would provide certain guiding significance for the de-velopment and ntilization of natural pigment.
