CAJ | 학술논문

目的：分析 HCV 核心蛋白（Core）和非结构蛋白（NS）5B 区直接测序的敏感性和准确性。方法收集慢性丙型肝炎患者血清51份，采用反转录 PCR 对 Core 和 NS5B 区分别扩增，产物直接测序，构建进化树进行基因分型。结果51份样本中，Core 区扩增阳性49份，占96．1％。共检出5种基因亚型：1b 为61．2％（30／49），2a 为20．4％（10／49），2b 为2．0％（1／49），3a 为4．1％（2／49），6a 为12．2％（6／49）。NS5B 区扩增阳性45份，占88．2％。1b、2a、2b、3a、6a 分别占62．2％（28／45）、20．0％（9／45）、2．2％（1／45）、4．4％（2／45）和11．1％（5／45）。结论与 NS5B 区相比，Core 区引物设计容易，扩增效率和分型成功率较高，可作为 HCV 基因型流行病学调查和临床研究的优先选择。
목적：분석 HCV 핵심단백（Core）화비결구단백（NS）5B 구직접측서적민감성화준학성。방법수집만성병형간염환자혈청51빈，채용반전록 PCR 대 Core 화 NS5B 구분별확증，산물직접측서，구건진화수진행기인분형。결과51빈양본중，Core 구확증양성49빈，점96．1％。공검출5충기인아형：1b 위61．2％（30／49），2a 위20．4％（10／49），2b 위2．0％（1／49），3a 위4．1％（2／49），6a 위12．2％（6／49）。NS5B 구확증양성45빈，점88．2％。1b、2a、2b、3a、6a 분별점62．2％（28／45）、20．0％（9／45）、2．2％（1／45）、4．4％（2／45）화11．1％（5／45）。결론여 NS5B 구상비，Core 구인물설계용역，확증효솔화분형성공솔교고，가작위 HCV 기인형류행병학조사화림상연구적우선선택。
Objective To explore the sensitivity and accuracy of directly sequenced core and non-structrural protein (NS)5B regions for hepatitis C virus (HCV)genotyping.Methods Fifty-one serum samples from chronic hepatitis C patients were collected in the study.Reverse transcription-polymerase chain reaction was used to amplify core and NS5B regions.Genotypes or subtypes were determined by the phylogenetic analysis of directly sequenced core and NS5B regions.Results Among the 51 samples,49 (96.1 %)were successfully typed by phylogenetic analysis of directly sequenced core region.There were overall five genotypes determined in the area,including 1b (61 .2%,30/49 ),2a (20.4%,10/49 ),2b (2.0%,1/49),3a (4.1 %,2/49 )and 6a (12.2%,6/49 ).The positive rate of HCV genotying was 88.2% (45/51 )on the basis of NS5B region.HCV genotypes 1b,2a,2b,3a and 6a were found in 62.2% (28/45),20.0% (9/45 ),2.2% (1/45 ),4.4% (2/45 )and 11 .1 % (5/45 )of the patients, respectively.Conclusion The HCV genotyping based on core regions,compared with that based on NS5B,shows the advantages of primer design,amplification efficiency and accuracy,suggesting that it has the priority to be used in the epidemiological and clinical study of HCV genotyping.