中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2015年
3期
222-225
,共4页
徐新宏%许林军%江璐%陈茜
徐新宏%許林軍%江璐%陳茜
서신굉%허림군%강로%진천
Novec 1230灭火剂%急性吸入%毒性%血常规指标%血液生化指标
Novec 1230滅火劑%急性吸入%毒性%血常規指標%血液生化指標
Novec 1230멸화제%급성흡입%독성%혈상규지표%혈액생화지표
Fire extinguishing agent novec 1230%Acute inhalation%Toxicity%Routine blood indexes%Blood biochemical indexes
目的:探讨Novec 1230灭火剂急性吸入对小鼠血常规和血液生化指标的影响。方法5周龄雄性ICR小鼠120只,体质量18~22 g,按数字表法随机分为染毒组(90只)和正常对照组(对照组,30只),染毒组各30只分别暴露于5%、10%、15%浓度的Novec 1230灭火剂气体中2 h,对照组暴露于普通室内空气中,每组分别设2个时间点(染毒2 h后、染毒2 h后正常饲养14 d),每个时间点15只,检测小鼠在这2个时间点的血常规和血液生化指标。结果在5%、10%、15%浓度染毒浓度下染毒2 h后,小鼠红细胞计数(RBC)[分别为(9.99±1.16)×1012、(9.90±0.68)×1012、(8.84±1.39)×1012/L]随染毒浓度增加逐渐下降,与对照组[(9.16±1.18)×1012/L]比较差异有统计学意义(P<0.05);白细胞计数(WBC)[分别为(4.11±0.75)×109、(6.23±1.05)×109、(6.51±1.30)×109/L]随染毒浓度增加逐渐上升,与对照组[(5.38±1.46)×109/L]比较差异有统计学意义(P<0.05或P<0.01);红细胞压积( HCT)、红细胞平均容积( MCV)、血小板计数( PLT)、血红蛋白( HGB)与对照组比较有不同程度的变化,但差异均无统计学意义(P >0.05);碱性磷酸酶(ALP)[分别为(218.67±0.85)、(210.13±0.76)、(196.07±6.63)U/L]随染毒浓度增加逐渐下降,丙氨酸氨基转移酶(ALT)[分别为(51.50±1.40)、(61.47±0.76)、(62.33±11.16)U/L]、天门冬氨酸氨基转移酶(AST)[分别为(195.03±1.60)、(201.30±6.56)、(259.43±11.82)U/L]、肌酸激酶(CK)[分别为(515.50±42.95)、(834.60±76.20)、(915.63±24.18)U/L]随染毒浓度增加逐渐上升,与对照组[分别为(184.97±2.46)、(39.37±0.97)、(187.70±2.51)、(452.20±93.01)U/L]比较差异均有统计学意义(P<0.05或P<0.01);饲养14 d后血常规和血液生化指标与对照组比较差异均无统计学意义(P>0.05)。结论5%、10%、15%浓度Novec 1230灭火剂气体急性吸入,能引起小鼠感染性损伤和心肌、肝脏损伤,但在脱离染毒环境14 d后损伤即可恢复。
目的:探討Novec 1230滅火劑急性吸入對小鼠血常規和血液生化指標的影響。方法5週齡雄性ICR小鼠120隻,體質量18~22 g,按數字錶法隨機分為染毒組(90隻)和正常對照組(對照組,30隻),染毒組各30隻分彆暴露于5%、10%、15%濃度的Novec 1230滅火劑氣體中2 h,對照組暴露于普通室內空氣中,每組分彆設2箇時間點(染毒2 h後、染毒2 h後正常飼養14 d),每箇時間點15隻,檢測小鼠在這2箇時間點的血常規和血液生化指標。結果在5%、10%、15%濃度染毒濃度下染毒2 h後,小鼠紅細胞計數(RBC)[分彆為(9.99±1.16)×1012、(9.90±0.68)×1012、(8.84±1.39)×1012/L]隨染毒濃度增加逐漸下降,與對照組[(9.16±1.18)×1012/L]比較差異有統計學意義(P<0.05);白細胞計數(WBC)[分彆為(4.11±0.75)×109、(6.23±1.05)×109、(6.51±1.30)×109/L]隨染毒濃度增加逐漸上升,與對照組[(5.38±1.46)×109/L]比較差異有統計學意義(P<0.05或P<0.01);紅細胞壓積( HCT)、紅細胞平均容積( MCV)、血小闆計數( PLT)、血紅蛋白( HGB)與對照組比較有不同程度的變化,但差異均無統計學意義(P >0.05);堿性燐痠酶(ALP)[分彆為(218.67±0.85)、(210.13±0.76)、(196.07±6.63)U/L]隨染毒濃度增加逐漸下降,丙氨痠氨基轉移酶(ALT)[分彆為(51.50±1.40)、(61.47±0.76)、(62.33±11.16)U/L]、天門鼕氨痠氨基轉移酶(AST)[分彆為(195.03±1.60)、(201.30±6.56)、(259.43±11.82)U/L]、肌痠激酶(CK)[分彆為(515.50±42.95)、(834.60±76.20)、(915.63±24.18)U/L]隨染毒濃度增加逐漸上升,與對照組[分彆為(184.97±2.46)、(39.37±0.97)、(187.70±2.51)、(452.20±93.01)U/L]比較差異均有統計學意義(P<0.05或P<0.01);飼養14 d後血常規和血液生化指標與對照組比較差異均無統計學意義(P>0.05)。結論5%、10%、15%濃度Novec 1230滅火劑氣體急性吸入,能引起小鼠感染性損傷和心肌、肝髒損傷,但在脫離染毒環境14 d後損傷即可恢複。
목적:탐토Novec 1230멸화제급성흡입대소서혈상규화혈액생화지표적영향。방법5주령웅성ICR소서120지,체질량18~22 g,안수자표법수궤분위염독조(90지)화정상대조조(대조조,30지),염독조각30지분별폭로우5%、10%、15%농도적Novec 1230멸화제기체중2 h,대조조폭로우보통실내공기중,매조분별설2개시간점(염독2 h후、염독2 h후정상사양14 d),매개시간점15지,검측소서재저2개시간점적혈상규화혈액생화지표。결과재5%、10%、15%농도염독농도하염독2 h후,소서홍세포계수(RBC)[분별위(9.99±1.16)×1012、(9.90±0.68)×1012、(8.84±1.39)×1012/L]수염독농도증가축점하강,여대조조[(9.16±1.18)×1012/L]비교차이유통계학의의(P<0.05);백세포계수(WBC)[분별위(4.11±0.75)×109、(6.23±1.05)×109、(6.51±1.30)×109/L]수염독농도증가축점상승,여대조조[(5.38±1.46)×109/L]비교차이유통계학의의(P<0.05혹P<0.01);홍세포압적( HCT)、홍세포평균용적( MCV)、혈소판계수( PLT)、혈홍단백( HGB)여대조조비교유불동정도적변화,단차이균무통계학의의(P >0.05);감성린산매(ALP)[분별위(218.67±0.85)、(210.13±0.76)、(196.07±6.63)U/L]수염독농도증가축점하강,병안산안기전이매(ALT)[분별위(51.50±1.40)、(61.47±0.76)、(62.33±11.16)U/L]、천문동안산안기전이매(AST)[분별위(195.03±1.60)、(201.30±6.56)、(259.43±11.82)U/L]、기산격매(CK)[분별위(515.50±42.95)、(834.60±76.20)、(915.63±24.18)U/L]수염독농도증가축점상승,여대조조[분별위(184.97±2.46)、(39.37±0.97)、(187.70±2.51)、(452.20±93.01)U/L]비교차이균유통계학의의(P<0.05혹P<0.01);사양14 d후혈상규화혈액생화지표여대조조비교차이균무통계학의의(P>0.05)。결론5%、10%、15%농도Novec 1230멸화제기체급성흡입,능인기소서감염성손상화심기、간장손상,단재탈리염독배경14 d후손상즉가회복。
Objective To investigate the effects of acute inhalation of fire extinguishing agent Novec 1230 on routine blood indexes and biochemical indexes in mice .Methods One hundred and twenty male ICR mice with an age range of 5 weeks and body mass of 18-22 g were randomly divided into the normal control group and the acute inhalation group , which were further divided into 3 subgroups , each consisting of 30 animals.The animals were exposed to the fire extinguishing agent Novec 1230 at concentration levels of 5%, 10%and 15%for 2 hours respectively , while the animals in the control group were exposed to common room air.Two time points ( at 2 hours and 14 days after inhalation exposure ) were set for each subgroups , which consisted of 15 mice.The routine blood levels and serum biochemical indexes of the mice were detected at the above 2 time points.Results Following exposure to the agent at levels of 5%, 10%and 15%for 2 hours, red blood cell (RBC) counts [(9.99 ±1.16) ×1012,(9.90 ±0.68) ×1012,(8.84 ±1.39) ×1012/L] decreased gradually with the increase in exposure concentrations , and statistical significance could be noted , when they were compared with those of the control group [(9.16 ±1.18)1012/L] (P<0.05).White blood cell (WBC) counts [(4.11 ±0.75) ×109,(6.23 ±1.05) ×109,(6.51 ±1.30) ×109/L] increased gradually with the increase in exposure concentrations , and statistical significance could be seen , when they were compared with those of the control group [(5.38 ±1.46) ×109/L] (P <0.05 or P <0.01).There were changes in hematocrit (HCT), mean corpuscular volume (MCV), platelet counts and hemoglobin (HGB), as compared with those of the control group , but without statistical significance (P<0.05).The levels of alkaline phosphate (ALP) [(218.67 ±0.85),(210.13 ±0.76)and(196.07 ±6.63)U/L] decreased gradually with the increase of exposure levels.The levels of alanine aminotransferase (ALT) were respectively [(51.50 ±1.40), (61.47 ±0.76) and (62.33 ±11.16)U/L], the levels of aspartate aminotransferase (AST) were respectively [(195.03 ±1.60)、(201.30 ±6.56)、(259.43 ±11.82)U/L] , and the levels of creatine kinase (CK) were respectively [(515.50 ±42.95),(834.60 ±76.20) and(915.63 ±24.18)U/L], which were increased with increase in exposure levels .Statistical significance could be observed , when they were compared with those of the control group [(184.97 ±2.46),(39.37 ±0.97),(187.70 ±2.51) and(452.20 ±93.01)U/L] (P<0. 05 or P<0.01).However,the differences in routine blood indexes and serum biochemical indexes were not statistically significant , when comparisons were made between the acute inhalation groups and the control group at day 14 after exposure(P>0.05).Conclusions Acute exposure of fire extinguishing agent Novec 1230 seemed to induce infectious damage , myocardial and heptal damage to mice , however , the damage afflicted on the animals seemed to recover 14 days after exposure .