CAJ | 학술논문

以蚕豆为试材，对甲醛（HCHO）处理0、24、48、72 h的蚕豆叶片进行气孔开度、导度、细胞内H2O2含量和抗氧化酶活性的测定，并用荧光显微检测法进行了H2O2的亚细胞定位检测。结果表明：气体HCHO处理的72 h内，由于蚕豆叶片超氧化物歧化酶（SOD）活性从284.52 U·min-1·g-1 FW升至291.44 U·min-1·g-1 FW，过氧化氢酶（CAT）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）活性先升高后降低，导致蚕豆叶片中H2O2含量从1.31μmol·g-1 FW升至2.39μmol·g-1 FW；较短时间（24~48 h）的HCHO处理下蚕豆叶片H2O2主要分布在保卫细胞的胞质中，72 h后不仅保卫细胞的胞质中H2O2积累增多，积累H2O2的叶绿体数量也增多；HCHO持续处理72 h内蚕豆叶片气孔开度和导度分别下降46.50％和78.80％，涂抹抗坏血酸（ASA）的实验证实，H2O2对蚕豆叶片气孔开度和导度的调节起到关键作用。研究认为，0.46~0.72 mg·m-3的HCHO胁迫致使蚕豆叶片中积累的H2O2定位于保卫细胞，是蚕豆叶片气孔开度减小和导度降低的主要原因。
이잠두위시재，대갑철（HCHO）처리0、24、48、72 h적잠두협편진행기공개도、도도、세포내H2O2함량화항양화매활성적측정，병용형광현미검측법진행료H2O2적아세포정위검측。결과표명：기체HCHO처리적72 h내，유우잠두협편초양화물기화매（SOD）활성종284.52 U·min-1·g-1 FW승지291.44 U·min-1·g-1 FW，과양화경매（CAT）、과양화물매（POD）、항배혈산과양화물매（APX）활성선승고후강저，도치잠두협편중H2O2함량종1.31μmol·g-1 FW승지2.39μmol·g-1 FW；교단시간（24~48 h）적HCHO처리하잠두협편H2O2주요분포재보위세포적포질중，72 h후불부보위세포적포질중H2O2적루증다，적루H2O2적협록체수량야증다；HCHO지속처리72 h내잠두협편기공개도화도도분별하강46.50％화78.80％，도말항배혈산（ASA）적실험증실，H2O2대잠두협편기공개도화도도적조절기도관건작용。연구인위，0.46~0.72 mg·m-3적HCHO협박치사잠두협편중적루적H2O2정위우보위세포，시잠두협편기공개도감소화도도강저적주요원인。
It has showed that formaldehyde(HCHO)concentrations in indoor air have elevated recently. Here we used Vicia faba as a test plant to examine the variations of the stomatal aperture and conductance, hydrogen peroxide content, and the activities of antioxidant en-zymes under HCHO stress for 0, 24, 48 h and 72 h. The subcellular localization of H2O2 was also measured by fluorescence microscopy. The activities of catalase(CAT), peroxidase(POD)and ascorbate peroxidase(APX)increased at the beginning but decreased afterward. Under HCHO stress for 72 h, superoxide dismutase(SOD)activity of V . faba leaves raised from 284.52 U·min-1·g-1 FW to 291.44 U·min-1·g-1 FW, resulting in increase of H2O2 content from 1.31μmol·g-1 FW to 2.39μmol·g-1 FW. Under 0~48 h of HCHO stress, H2O2 in V . faba leaves was mainly distributed in the cytoplasm of guard cells, whereas H2O2 accumulated in both the cytoplasm and the chloroplast of the guard cells after 72 h. The stomatal aperture and conductance decreased by 46.50% and 78.80%, respectively, after continuous HCHO stress for 72 h. Ascorbic acid(ASA)-daubed experiment confirmed that H2O2 played a critical role in regulating the stomatal aperture and conductance of the plant. This research indicates that H2O2 is localized in guard cells of V . faba leaves under HCHO stress of 0.46~0.72 mg· m-3, which effectively inhibits the stomatal aperture and conductance of V . faba leaves and thus protects plant from HCHO stresses.