广西植物
廣西植物
엄서식물
GUIHAIA
2015年
4期
598-603
,共6页
孙雪丹%何苗%罗友%刘铁秋%卢勇%曾宪垠
孫雪丹%何苗%囉友%劉鐵鞦%盧勇%曾憲垠
손설단%하묘%라우%류철추%로용%증헌은
石菖蒲%番茄%防御酶%GC-MS%β-细辛醚
石菖蒲%番茄%防禦酶%GC-MS%β-細辛醚
석창포%번가%방어매%GC-MS%β-세신미
Acorus tatarinowii%tomato%defensive enzymes%GC-MS%β-asarone
为了探讨石菖蒲醇提物作为一种中药杀菌剂对病菌寄主植物本身防御酶体系的影响,该研究以番茄欧粉贝幼苗和川产道地中药石菖蒲为材料,采用冷浸法制备石菖蒲醇提物,经不同浓度石菖蒲醇提物对番茄幼苗进行处理,用比色法测定多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性,并利用GC-MS 联用技术,对石菖蒲醇提物的主要化学成分进行定性分析.结果表明：第1天,醇提物浓度在0．04~0．08 g·mL-1时,PAL 活性显著高于对照；随后 PAL 活性随浓度升高降低；当浓度在0．04~0．16 g·mL-1时, POD 活性与对照无显著差异；浓度为0．32 g·mL-1时,POD 活性显著高于对照；浓度为0．64 g·mL-1时,POD活性显著低于对照；当浓度在0．04~0．32 g·mL-1时,PPO 活性显著高于对照；浓度为0．64 g·mL-1时,PPO活性与对照无显著差异；随着时间的延长,三种酶各处理组之间的差异逐步减少.第4天时,各处理组之间PAL 的活性无显著差异；当浓度为0．04 g·mL-1和0．64 g·mL-1时,PPO 活性与对照无显著差异,其余范围均显著高于对照；当浓度在0．08~0．32 g·mL-1时,POD 活性数值上均高于对照.以上表明在一定浓度范围内醇提物可以增强防御酶活性,未破坏植物的防御酶体系.因此,该醇提物作为一种新型的中药杀菌剂有巨大的开发和利用潜力.利用 GC-MS 联用技术,可从醇提物中鉴定出30种化学成分,这些物质中多为苯丙素类物质,如β-细辛醚、α-细辛醚和顺式甲基异丁香油酚等,其中含量最高的物质为β-细辛醚(42．48％).这提示在后续的研究中,可针对β-细辛醚等醇提物的主要化学成分进行.
為瞭探討石菖蒲醇提物作為一種中藥殺菌劑對病菌寄主植物本身防禦酶體繫的影響,該研究以番茄歐粉貝幼苗和川產道地中藥石菖蒲為材料,採用冷浸法製備石菖蒲醇提物,經不同濃度石菖蒲醇提物對番茄幼苗進行處理,用比色法測定多酚氧化酶(PPO)、苯丙氨痠解氨酶(PAL)和過氧化物酶(POD)的活性,併利用GC-MS 聯用技術,對石菖蒲醇提物的主要化學成分進行定性分析.結果錶明：第1天,醇提物濃度在0．04~0．08 g·mL-1時,PAL 活性顯著高于對照；隨後 PAL 活性隨濃度升高降低；噹濃度在0．04~0．16 g·mL-1時, POD 活性與對照無顯著差異；濃度為0．32 g·mL-1時,POD 活性顯著高于對照；濃度為0．64 g·mL-1時,POD活性顯著低于對照；噹濃度在0．04~0．32 g·mL-1時,PPO 活性顯著高于對照；濃度為0．64 g·mL-1時,PPO活性與對照無顯著差異；隨著時間的延長,三種酶各處理組之間的差異逐步減少.第4天時,各處理組之間PAL 的活性無顯著差異；噹濃度為0．04 g·mL-1和0．64 g·mL-1時,PPO 活性與對照無顯著差異,其餘範圍均顯著高于對照；噹濃度在0．08~0．32 g·mL-1時,POD 活性數值上均高于對照.以上錶明在一定濃度範圍內醇提物可以增彊防禦酶活性,未破壞植物的防禦酶體繫.因此,該醇提物作為一種新型的中藥殺菌劑有巨大的開髮和利用潛力.利用 GC-MS 聯用技術,可從醇提物中鑒定齣30種化學成分,這些物質中多為苯丙素類物質,如β-細辛醚、α-細辛醚和順式甲基異丁香油酚等,其中含量最高的物質為β-細辛醚(42．48％).這提示在後續的研究中,可針對β-細辛醚等醇提物的主要化學成分進行.
위료탐토석창포순제물작위일충중약살균제대병균기주식물본신방어매체계적영향,해연구이번가구분패유묘화천산도지중약석창포위재료,채용랭침법제비석창포순제물,경불동농도석창포순제물대번가유묘진행처리,용비색법측정다분양화매(PPO)、분병안산해안매(PAL)화과양화물매(POD)적활성,병이용GC-MS 련용기술,대석창포순제물적주요화학성분진행정성분석.결과표명：제1천,순제물농도재0．04~0．08 g·mL-1시,PAL 활성현저고우대조；수후 PAL 활성수농도승고강저；당농도재0．04~0．16 g·mL-1시, POD 활성여대조무현저차이；농도위0．32 g·mL-1시,POD 활성현저고우대조；농도위0．64 g·mL-1시,POD활성현저저우대조；당농도재0．04~0．32 g·mL-1시,PPO 활성현저고우대조；농도위0．64 g·mL-1시,PPO활성여대조무현저차이；수착시간적연장,삼충매각처리조지간적차이축보감소.제4천시,각처리조지간PAL 적활성무현저차이；당농도위0．04 g·mL-1화0．64 g·mL-1시,PPO 활성여대조무현저차이,기여범위균현저고우대조；당농도재0．08~0．32 g·mL-1시,POD 활성수치상균고우대조.이상표명재일정농도범위내순제물가이증강방어매활성,미파배식물적방어매체계.인차,해순제물작위일충신형적중약살균제유거대적개발화이용잠력.이용 GC-MS 련용기술,가종순제물중감정출30충화학성분,저사물질중다위분병소류물질,여β-세신미、α-세신미화순식갑기이정향유분등,기중함량최고적물질위β-세신미(42．48％).저제시재후속적연구중,가침대β-세신미등순제물적주요화학성분진행.
This paper aims to explore Acorus tatarinowii alcohol extracts as a kind of traditional Chinese herbal medi-cine fungicide effects on the plant defense enzyme system of fungi host.The test materials were tomato seedlings and Sichuan authentic herbal medicine A .tatarinowii .A .tatarinowii alcohol extracts were prepared by cold-maceration. The main experimental methods were colorimetric method and gas chromatography-mass spectrometry.The activity of plant defensive enzymes were examined by colorimetric method after the treatment of tomato seedlings using dif-ferent concentrations of alcoholic extracts from A .tatarinowii .And the main chemical compositions of A .tatarinowii alcohol extracts were analyzed by gas chromatography-mas s spectrometry.These results showed that when the concentration ofA .tatarinowii alcohol extracts in 0.04-0.08 g·mL-1 ,the activity of PAL was significantly higher than control groups on the first day.Then the activity of PAL reduced with the increase of the concentration of alcohol extract.And at the outset of the activity of POD had no significant difference on concentration condition of 0.04-0.16 g·mL-1 .When the concentration ofA .tatarinowii alcohol extracts in 0.64 g·mL-1 ,the activity of PAL was significantly lower than control groups .And the activity of PPO was significantly higher than control groups on concentration condition of 0.04-0.32 g·mL-1 .When in 0.64 g·mL-1 ,the activity of PPO had no significant difference with control groups.But with the extension of time,the difference between each group of three enzymes was reduced. On the 4th day,the activity of PAL had no significant differences between treatment groups.When the concentration of alcohol extracts in 0.04-0.64 g·mL-1 ,the activity of PPO had no significant differences with control groups.The activity of PPO was significantly higher than control groups on the scope of the other.When in 0.08-0.32 g·mL-1 , the activity of POD of values were higher than those in control groups.The results showed thatA .tatarinowii alcohol extracts could influence the activity of plant defensive enzymes and a certain concentration ofA .tatarinowii alcohol extracts could enhance it without damaging plants defense enzyme system.Therefore,A .tatarinowii alcohol extracts as a new type of traditional Chinese herbal medicine fungicide is a huge potential of development and utilization. And thirty chemical compositions were identified fromA .tatarinowii alcoholic extracts by gas chromatography-mass spectrometry.The main chemical composition ofA .tatarinowii alcohol extracts was phenylpropanoids such asβ-asarone,α-asarone and cis-Methylisoeugenol.The most abundant chemical composition wasβ-asarone.And the content ofβ-asarone inA .tatarinowii alcohol extracts accounted for 42.48%.And in follow-up studies,we can study the main chemical composition ofA .tatarinowii alcohol extracts likeβ-asarone.
