CAJ | 학술논문

从制药厂的活性污泥中分离到一株能以唯一碳源和硫源降解甲硫醚的菌株JLM-8，经过生理生化测试与16S rDNA系统发育树分析鉴定为嗜麦芽寡养单胞菌（Stenotrophomonasmaltophilia）。通过测定菌株的生长量、甲硫醚的降解率，利用响应面法优化最佳降解条件，并测定了该菌降解甲硫醚的动力学参数。结果表明：当接种量为25 mg·L?1时，通过响应面法优化的最佳降解条件为温度31.3℃、pH 7.5，初始甲硫醚浓度50 mg·L?1时最大预测降解率为98.2%，实验验证降解率为97.9%。菌株降解动力学参数最大比降解速率、半饱和系数、抑制系数分别为2.37 h?1、143.55 mg·L?1、51.35 mg·L?1，临界抑制浓度为78.46 mg·L?1。
종제약엄적활성오니중분리도일주능이유일탄원화류원강해갑류미적균주JLM-8，경과생리생화측시여16S rDNA계통발육수분석감정위기맥아과양단포균（Stenotrophomonasmaltophilia）。통과측정균주적생장량、갑류미적강해솔，이용향응면법우화최가강해조건，병측정료해균강해갑류미적동역학삼수。결과표명：당접충량위25 mg·L?1시，통과향응면법우화적최가강해조건위온도31.3℃、pH 7.5，초시갑류미농도50 mg·L?1시최대예측강해솔위98.2%，실험험증강해솔위97.9%。균주강해동역학삼수최대비강해속솔、반포화계수、억제계수분별위2.37 h?1、143.55 mg·L?1、51.35 mg·L?1，림계억제농도위78.46 mg·L?1。
Dimethyl sulfide (DMS) is one of the most important volatile organic sulfur compounds. Due to its malodorous smell and the extremely low odor threshold, DMS has become a critical issue in wastewater collection and treatment systems. In this study, a novel strain JLM-8, with capability of degrading DMS as sole carbon and sulfur source, was isolated from the active sludge of a pharmaceutical plant. Based on the morphological, physiological and biochemical properties as well as its 16S rDNA gene sequence analysis, the strain JLM-8 was identified as a member of Stenotrophomonasmaltophilia. The strain could degrade approximately 100% of 50 mg·L?1 DMS within 8 hours at pH 7 and 30℃. Response surface methodology (RSM) analysis showed that the optimum conditions for degradation were at pH 7.5 and 31.3℃, using an inoculum amount of 25 mg·L?1. Under these conditions, approximately 97.9% of DMS was degraded. The kinetic parametersqmax,Ks, andKi were established to be 2.37 h?1, 143.55 mg·L?1, 51.35 mg·L?1, respectively. The critical inhibitor concentration was determined to be 78.46 mg·L?1. Research on the characterization of DMS degradable bacteria can provide a new theoretical and technical fundamental basis for bioremediation of DMS-contaminated environments.