CAJ | 학술논문

目的：探讨男性不育患者精浆弹性硬蛋白酶水平与精子 DNA完整性及精液质量之间的关系。方法选择148例男性不育患者根据精浆弹性硬蛋白酶的检测结果将所有患者分为 A、B、C3组。 A组为精浆弹性硬蛋白酶含量＜290 ng／mL；B组为精浆弹性硬蛋白酶含量290～1000 ng／mL；C 组为精浆弹性硬蛋白酶含量＞1000ng／mL。用酶联免疫吸附试验（ ELISA）检测精浆中弹性硬蛋白酶，精子 DNA完整性检测用精子染色质扩散法（ SCD），精液参数用计算机辅助精子分析系统检测。结果 A组与 C 组相比精子存活率与前向运动精子百分率（PR）均升高、精子DNA碎片指数（DFI）明显降低，差异均具有统计学意义（ P＜0．05）。 B组的上述指标与A组相比，差异均无统计学意义（ P ＞0．05）。结论精浆弹性硬蛋白酶与精子DNA完整性及精液质量密切相关，生殖道感染是影响男性精液质量的一个重要原因。
목적：탐토남성불육환자정장탄성경단백매수평여정자 DNA완정성급정액질량지간적관계。방법선택148례남성불육환자근거정장탄성경단백매적검측결과장소유환자분위 A、B、C3조。 A조위정장탄성경단백매함량＜290 ng／mL；B조위정장탄성경단백매함량290～1000 ng／mL；C 조위정장탄성경단백매함량＞1000ng／mL。용매련면역흡부시험（ ELISA）검측정장중탄성경단백매，정자 DNA완정성검측용정자염색질확산법（ SCD），정액삼수용계산궤보조정자분석계통검측。결과 A조여 C 조상비정자존활솔여전향운동정자백분솔（PR）균승고、정자DNA쇄편지수（DFI）명현강저，차이균구유통계학의의（ P＜0．05）。 B조적상술지표여A조상비，차이균무통계학의의（ P ＞0．05）。결론정장탄성경단백매여정자DNA완정성급정액질량밀절상관，생식도감염시영향남성정액질량적일개중요원인。
Objectives To study the relationship of the seminal plasma elastase(SPE) level with sperm DNA integrity and semen quality in infertile men.Methods Based on the results of seminal plasma elastase tests, 148 infertile men were divided into group A(SPE:<290 ng/mL), group B( SPE:290~1000ng/mL),and groupC( SPE:>1000ng/mL).The level of SPE was detected by ELISA,integrity of sperm DNA weew used sperm chromation dispersion test to measure, and analyses were also performed on the semen parameters by CASA.Results Sperm livability and the rate of progressively motile sperm(PR) in group A were all higher than that in group C,and the DFI was apparently depressed( P <0.05).But there was no significant difference in the indexes above between group B and group A( P >0.05).Conclusions The results suggested that seminal plasma elastase was closely associat-ed with sperm DNA integrity and semen quality,meanwhile the reproductive tract infection is an important factor af-fecting the male semen quality.