CAJ | 학술논문

目的：运用代谢组学技术和放射免疫技术，观察加速度暴露对人体血液代谢物、应激激素和抵抗素、瘦素等胃肠道调节因子的含量影响，为阐明晕动病胃肠道症状的发生机制提供新的线索。方法运用六自由度舰船运动模拟装置对招募到的60名大学生进行加速度暴露15 min，采用格瑞比尔评分法进行晕动病严重程度评分，采用气相色谱法耦合飞行时间质谱分析（ GC-TOF／MS）技术检测血液代谢物的水平，运用放免法、ELISA等技术检测血液糖皮质激素、肾上腺素、精氨酸加压素、胰岛素和抵抗素、瘦素等的含量。结果（1）晕动病发生率为72％；（2）加速度暴露可以引起受试者血清代谢物含量发生明显变化，其中葡萄糖和N-月桂酸含量明显升高，L-丝氨酸，L-苏氨酸明显下降（P＜0．05）；（3）加速度暴露可导致血清胰岛素下降[暴露前（21．03±6．78）mIU／L，暴露后（14．97±2．94）mIU／L]，胰高血糖素、糖皮质激素、肾上腺素、精氨酸加压素升高[暴露前分别为（18．06±5．72） ng／L、（212．88±61．37）μg／L、（88．41±35．72）ng／L、（10．80±7．30）ng／L，暴露后分别为（20．40±5．48）ng／L、（293．34±50．91）μg／L、（132．83±67．25）ng／L、（51．35±8．24）ng／L]（P＜0．05或P＜0．001）；（4）加速度暴露可引起肠抑胃肽、瘦素和血清抵抗素水平明显降低，胃饥饿素水平明显升高[暴露前分别为（170．34±82．64）nmol／L、（744．03±438．46）μg／L、（6687．96±3289．18）μg／L、（136．43±74．75）ng／L，暴露后分别为（41．05±17．91） nmol／L、（409．95±347．95）μg／L、（2615．56±1345．61）μg／L、（210．85±109．01） ng／L]（ P＜0．05或P＜0．001）。结论加速度暴露可以引起机体发生应激反应，导致血清应激激素水平和部分胃肠道调节因子以及代谢物含量明显变化。目的：運用代謝組學技術和放射免疫技術，觀察加速度暴露對人體血液代謝物、應激激素和牴抗素、瘦素等胃腸道調節因子的含量影響，為闡明暈動病胃腸道癥狀的髮生機製提供新的線索。方法運用六自由度艦船運動模擬裝置對招募到的60名大學生進行加速度暴露15 min，採用格瑞比爾評分法進行暈動病嚴重程度評分，採用氣相色譜法耦閤飛行時間質譜分析（ GC-TOF／MS）技術檢測血液代謝物的水平，運用放免法、ELISA等技術檢測血液糖皮質激素、腎上腺素、精氨痠加壓素、胰島素和牴抗素、瘦素等的含量。結果（1）暈動病髮生率為72％；（2）加速度暴露可以引起受試者血清代謝物含量髮生明顯變化，其中葡萄糖和N-月桂痠含量明顯升高，L-絲氨痠，L-囌氨痠明顯下降（P＜0．05）；（3）加速度暴露可導緻血清胰島素下降[暴露前（21．03±6．78）mIU／L，暴露後（14．97±2．94）mIU／L]，胰高血糖素、糖皮質激素、腎上腺素、精氨痠加壓素升高[暴露前分彆為（18．06±5．72） ng／L、（212．88±61．37）μg／L、（88．41±35．72）ng／L、（10．80±7．30）ng／L，暴露後分彆為（20．40±5．48）ng／L、（293．34±50．91）μg／L、（132．83±67．25）ng／L、（51．35±8．24）ng／L]（P＜0．05或P＜0．001）；（4）加速度暴露可引起腸抑胃肽、瘦素和血清牴抗素水平明顯降低，胃饑餓素水平明顯升高[暴露前分彆為（170．34±82．64）nmol／L、（744．03±438．46）μg／L、（6687．96±3289．18）μg／L、（136．43±74．75）ng／L，暴露後分彆為（41．05±17．91） nmol／L、（409．95±347．95）μg／L、（2615．56±1345．61）μg／L、（210．85±109．01） ng／L]（ P＜0．05或P＜0．001）。結論加速度暴露可以引起機體髮生應激反應，導緻血清應激激素水平和部分胃腸道調節因子以及代謝物含量明顯變化。
목적：운용대사조학기술화방사면역기술，관찰가속도폭로대인체혈액대사물、응격격소화저항소、수소등위장도조절인자적함량영향，위천명훈동병위장도증상적발생궤제제공신적선색。방법운용륙자유도함선운동모의장치대초모도적60명대학생진행가속도폭로15 min，채용격서비이평분법진행훈동병엄중정도평분，채용기상색보법우합비행시간질보분석（ GC-TOF／MS）기술검측혈액대사물적수평，운용방면법、ELISA등기술검측혈액당피질격소、신상선소、정안산가압소、이도소화저항소、수소등적함량。결과（1）훈동병발생솔위72％；（2）가속도폭로가이인기수시자혈청대사물함량발생명현변화，기중포도당화N-월계산함량명현승고，L-사안산，L-소안산명현하강（P＜0．05）；（3）가속도폭로가도치혈청이도소하강[폭로전（21．03±6．78）mIU／L，폭로후（14．97±2．94）mIU／L]，이고혈당소、당피질격소、신상선소、정안산가압소승고[폭로전분별위（18．06±5．72） ng／L、（212．88±61．37）μg／L、（88．41±35．72）ng／L、（10．80±7．30）ng／L，폭로후분별위（20．40±5．48）ng／L、（293．34±50．91）μg／L、（132．83±67．25）ng／L、（51．35±8．24）ng／L]（P＜0．05혹P＜0．001）；（4）가속도폭로가인기장억위태、수소화혈청저항소수평명현강저，위기아소수평명현승고[폭로전분별위（170．34±82．64）nmol／L、（744．03±438．46）μg／L、（6687．96±3289．18）μg／L、（136．43±74．75）ng／L，폭로후분별위（41．05±17．91） nmol／L、（409．95±347．95）μg／L、（2615．56±1345．61）μg／L、（210．85±109．01） ng／L]（ P＜0．05혹P＜0．001）。결론가속도폭로가이인기궤체발생응격반응，도치혈청응격격소수평화부분위장도조절인자이급대사물함량명현변화。
Objective Metabolomics and radioimmunoassay technologies were used to analyze the effects of acceleration exposure on the levels of human serum metabolites , stress-related hormones and such gastrointestinal regulatory factors as resistin and leptin , so as to provide new scientific evidence for the mechanism of gastrointestinal symptoms in motion sickness .Methods Sixty volunteers were recruited and exposed to abnormal acceleration for 15 minutes by using a 6-degree-of-freedom ship motion simulator .The severity of motion sickness was measured by Graybiel Scale; gas chromatography coupled with time-of-flight mass spectrometry ( GC-TOF/MS ) was used to detect serum metabolites in the human subjects; serum glucocorticoid, adrenaline, arginine vasopressin ( AVP ), insulin, resistin and leptin were detected by radioimmunoassay and ELISA .Results ( 1 ) The incidence of motion sickness was 72%.( 2 ) Acceleration exposure could induce significant changes in the levels of serum metabolites in the subjects , of which glucose and N-dodecanoic acid levels were significantly higher than those before exposure , while L-serine and L-threonine levels were significantly decreased after exposure (P<0.05).(3) Acceleration exposure could cause decrease in the level of serum insulin [ (21.03 ±6.78) mIU/L before exposure,(14.97 ±2.94)mIU/L after exposure], and increase in the level of glucagon , glucocorticoid, epinephrine and arginine vasopressin (AVP) [ (18.06 ±5.72)ng/L,(212.88 ±61.37)μg/L,(88.41 ±35.72)ng/L and(10.80 ±7.30)ng/L before exposure;(20.40 ±5.48)ng/L,(293.34 ±50.91)μg/L,(132.83 ±67.25 )ng/L and(51.35 ±8.24)ng/L after exposure] respectively(P<0.05 or P<0.001).(4) Acceleration exposure could also decrease the levels of gastric inhibitory peptide ( GIP), leptin,resistin, but increase the level of ghrelin [ (170.34 ±82.64) nmol/L,(744.03 ±438.46)μg/L,(6687.96 ±3289.18)μg/L and (136.43 ±74.75) ng/L before exposure;(41.05 ±17.91) nmol/L,(409.95 ±347.95)μg/L,(2615.56 ±1345.61)μg/L and (210.85 ±109.01) ng/L after exposure] respectively(P<0.05 or P<0.01).Conclusions Our study indicated that stress could be induced by acceleration exposure , resulting in significant changes in the levels of stress-related hormones and gastrointestinal regulatory factors , as well as serum metabolites .