CAJ | 학술논문

在模拟生理条件下，借助荧光光谱研究了磺胺二甲基嘧啶（SMZ）与牛血清白蛋白（BSA）以及Eu（III）对磺胺二甲基嘧啶与牛血清白蛋白相互作用的光谱行为。求算了不同温度下（275 K和308 K）相互作用的结合常数Kb 和结合位点数n。结果表明：磺胺二甲基嘧啶对牛血清白蛋白荧光有猝灭，其过程属于静态猝灭机理。 Eu（Ⅲ）的存在使SMZ与BSA的结合常数降低，结合位点数变小。
재모의생리조건하，차조형광광보연구료광알이갑기밀정（SMZ）여우혈청백단백（BSA）이급Eu（III）대광알이갑기밀정여우혈청백단백상호작용적광보행위。구산료불동온도하（275 K화308 K）상호작용적결합상수Kb 화결합위점수n。결과표명：광알이갑기밀정대우혈청백단백형광유졸멸，기과정속우정태졸멸궤리。 Eu（Ⅲ）적존재사SMZ여BSA적결합상수강저，결합위점수변소。
The interaction between sulfamethazine and bovine serum albumin, and the influence of Eu(Ⅲ) on the system of sulfamethazine and bovine serum albumin was studied under the physiological conditions by fluorescence method.The binding constant Kb and the number of binding sites n were measured at diffrent temperatures (275 K and 308 K) by fluorescence quenching method.The results showed that sulfanilamide dimethyl pyridine could quench the fluorescence of bovine serum albumin, and it was the static quenching.The binding constant and the number of binding sites of both compounds decreased in the presence of Eu(Ⅲ).