CAJ | 학술논문

基于紫杉醇的抗癌功效，探究何种条件（乙醇浓度，固液比，提取时间，提取次数，提取功率，温度，颗粒直径，溶媒，提取方法，提取部位）下，能在有限的红豆杉中最大量的提取紫杉醇很有必要。在单因素条件的基础上，选取预处理中影响紫杉醇提取的4个因素（固液比，提取时间，超声波功率，温度）进行4因素3水平（L9（34））正交实验。然后对K值进行分析，得出结论：最优的超声波预处理实验组合为温度50℃、固液比为1：35、超声波提取时间为40 min、超声波功率为70％。最后采取氧化铝柱层析进一步除杂，通过高效液相色谱结果，对提取纯化的效果进行分析。
기우자삼순적항암공효，탐구하충조건（을순농도，고액비，제취시간，제취차수，제취공솔，온도，과립직경，용매，제취방법，제취부위）하，능재유한적홍두삼중최대량적제취자삼순흔유필요。재단인소조건적기출상，선취예처리중영향자삼순제취적4개인소（고액비，제취시간，초성파공솔，온도）진행4인소3수평（L9（34））정교실험。연후대K치진행분석，득출결론：최우적초성파예처리실험조합위온도50℃、고액비위1：35、초성파제취시간위40 min、초성파공솔위70％。최후채취양화려주층석진일보제잡，통과고효액상색보결과，대제취순화적효과진행분석。
based on Paclitaxel anti-cancer efficacy, it is necessary to find out that, under which conditions a large number of Paclitaxel can be extracted from Taxus chinensis, such as ethanol concentration, solid-liquid ratio, extraction time, extraction times, extraction power, temperature, particle diameter, solvent, extraction method, extraction parts, etc.Based on single factor conditions, 4 factors, solid-liquid ratio, extraction time, ultrasonic power, temperature, which influenced the selection of taxol extraction pretreatment, were used in L9(34 ) orthogonal experiment.Analyzing the K value, it was concluded that the best optimal ultrasonic pretreatment experiment combination was as follows: the temperature was 50℃, the ratio of solid to liquid was 1:35, ultrasonic extraction time was 40 min, ultrasonic power was 70%. Using alumina column chromatography to impurities further removement, under the high performance liquid chromatography results, the effect of the extraction and purification was analyzed.