CAJ | 학술논문

目的：探讨番茄红素对PM2．5暴露内皮细胞氧化损伤的分子机制。方法利用CCK－8明确番茄红素减轻 PM2．5暴露内皮细胞损伤的最适宜浓度；检测细胞丙二醛（ MDA）含量及超氧化物歧化酶（ SOD）活性，评估番茄红素解除PM2．5对内皮细胞氧化损伤的作用；Western 印迹检测细胞内缺氧诱导因子（HIF）－1α表达；q－PCR、ELISA检测细胞白介素（IL）－6表达水平。结果解除因PM2．5暴露造成氧化损伤的最佳番茄红素浓度为80μg／ml；番茄红素作用使PM2．5暴露内皮细胞氧化应激指标SOD活性升高48．04％，MDA含量下降38．89％，IL－6、HIF－1α恢复到正常细胞水平。结论番茄红素具有解除PM2．5暴露内皮细胞氧化损伤的作用。
목적：탐토번가홍소대PM2．5폭로내피세포양화손상적분자궤제。방법이용CCK－8명학번가홍소감경 PM2．5폭로내피세포손상적최괄의농도；검측세포병이철（ MDA）함량급초양화물기화매（ SOD）활성，평고번가홍소해제PM2．5대내피세포양화손상적작용；Western 인적검측세포내결양유도인자（HIF）－1α표체；q－PCR、ELISA검측세포백개소（IL）－6표체수평。결과해제인PM2．5폭로조성양화손상적최가번가홍소농도위80μg／ml；번가홍소작용사PM2．5폭로내피세포양화응격지표SOD활성승고48．04％，MDA함량하강38．89％，IL－6、HIF－1α회복도정상세포수평。결론번가홍소구유해제PM2．5폭로내피세포양화손상적작용。
Objective To study the protective effect and find out the molecular mechanism of lycopene on damaged endothelial cells exposed to PM2.5.Methods The optimum concentration of lycopene was found , the endothelial cells exposed to 80 μg/ml PM2.5 were administrated by series of concentrations (0, 40, 80, 160μg/ml), cell viability was assayed by CCK-8 kits;MDA content and SOD activity were elevated respectively .HIF-1αexpression was detected by Western blot;IL-6 concentration was assayed by q-PCR and ELISA .Results The concentration of 80μg/ml lycopene rescued oxidative damage on endothelial cells exposed to 80μg/ml PM2.5.Moreover, SOD activ-ity was significantly increased 48.04%(P<0.05) and MDA concentration was significantly declined 38.89% (P<0.05) post-administra-tion;Furthermore, IL-6 and HIF-1αlevels in endothelial cells exposured to PM 2.5 were declined by lycopene .Conclusions Lycopene could rescue endothelial cells oxidative damage by exposure to PM 2.5.