中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2015年
3期
184-186,217
,共4页
缺氧缺血性脑病%高压氧%新生儿%丙二醛%超氧化物歧化酶%神经元特异性烯醇化酶%髓鞘碱性蛋白
缺氧缺血性腦病%高壓氧%新生兒%丙二醛%超氧化物歧化酶%神經元特異性烯醇化酶%髓鞘堿性蛋白
결양결혈성뇌병%고압양%신생인%병이철%초양화물기화매%신경원특이성희순화매%수초감성단백
Hypoxic ischemic encephalopathy%Hyperbaric oxygen%Neonate%Malondialdehyde%Superoxide dismutase%Neuron specific enolase%Myelin basic protein
目的：探讨高压氧治疗对缺氧缺血性脑病（ HIE）新生儿血清丙二醛（ MDA）、超氧化物歧化酶（ SOD）、神经元特异性烯醇化酶（ NSE）及血清髓鞘碱性蛋白（ MBP）水平的影响。方法90例HIE足月新生儿，按治疗方法分为高压氧组45例，常规治疗组45例，选取同期出生的正常新生儿45例为正常对照组。在治疗前后分别用酶联免疫分析法及化学比色法检测血清MDA、SOD、NSE、MBP水平。评估患儿治疗前后行为神经评分（ NBNA）变化，并分析NBNA与以上指标的相关性。结果 HIE患儿治疗前MDA、NSE、MBP水平明显高于正常对照组[（4．48±0．94） nmol／L、（10．02±3．10）μg／L、（4．58±2．17）μg／L]（P＜0．05），SOD明显低于正常对照组[（137．65±34．54）μU／L]（P＜0．05）；高压氧组和常规治疗组治疗后血清SOD上升，MDA、NSE、MBP下降，NBNA评分升高，较同组治疗前差异有统计学意义（P＜0．05）；高压氧组中／重度HIE患儿上述指标变化更明显，与相同程度常规治疗组比较差异有统计学意义（P＜0．01）；MBP与NBNA之间具有相关性。结论高压氧治疗提高HIE患儿抗氧化能力，降低脑组织脂质过氧化反应损伤，联合检测MDA、SOD、NSE和MBP有利于病情的评估和判断。
目的：探討高壓氧治療對缺氧缺血性腦病（ HIE）新生兒血清丙二醛（ MDA）、超氧化物歧化酶（ SOD）、神經元特異性烯醇化酶（ NSE）及血清髓鞘堿性蛋白（ MBP）水平的影響。方法90例HIE足月新生兒，按治療方法分為高壓氧組45例，常規治療組45例，選取同期齣生的正常新生兒45例為正常對照組。在治療前後分彆用酶聯免疫分析法及化學比色法檢測血清MDA、SOD、NSE、MBP水平。評估患兒治療前後行為神經評分（ NBNA）變化，併分析NBNA與以上指標的相關性。結果 HIE患兒治療前MDA、NSE、MBP水平明顯高于正常對照組[（4．48±0．94） nmol／L、（10．02±3．10）μg／L、（4．58±2．17）μg／L]（P＜0．05），SOD明顯低于正常對照組[（137．65±34．54）μU／L]（P＜0．05）；高壓氧組和常規治療組治療後血清SOD上升，MDA、NSE、MBP下降，NBNA評分升高，較同組治療前差異有統計學意義（P＜0．05）；高壓氧組中／重度HIE患兒上述指標變化更明顯，與相同程度常規治療組比較差異有統計學意義（P＜0．01）；MBP與NBNA之間具有相關性。結論高壓氧治療提高HIE患兒抗氧化能力，降低腦組織脂質過氧化反應損傷，聯閤檢測MDA、SOD、NSE和MBP有利于病情的評估和判斷。
목적：탐토고압양치료대결양결혈성뇌병（ HIE）신생인혈청병이철（ MDA）、초양화물기화매（ SOD）、신경원특이성희순화매（ NSE）급혈청수초감성단백（ MBP）수평적영향。방법90례HIE족월신생인，안치료방법분위고압양조45례，상규치료조45례，선취동기출생적정상신생인45례위정상대조조。재치료전후분별용매련면역분석법급화학비색법검측혈청MDA、SOD、NSE、MBP수평。평고환인치료전후행위신경평분（ NBNA）변화，병분석NBNA여이상지표적상관성。결과 HIE환인치료전MDA、NSE、MBP수평명현고우정상대조조[（4．48±0．94） nmol／L、（10．02±3．10）μg／L、（4．58±2．17）μg／L]（P＜0．05），SOD명현저우정상대조조[（137．65±34．54）μU／L]（P＜0．05）；고압양조화상규치료조치료후혈청SOD상승，MDA、NSE、MBP하강，NBNA평분승고，교동조치료전차이유통계학의의（P＜0．05）；고압양조중／중도HIE환인상술지표변화경명현，여상동정도상규치료조비교차이유통계학의의（P＜0．01）；MBP여NBNA지간구유상관성。결론고압양치료제고HIE환인항양화능력，강저뇌조직지질과양화반응손상，연합검측MDA、SOD、NSE화MBP유리우병정적평고화판단。
Objective To explore the effects of hyperbaric oxygen ( HBO) therapy on the serum levels of malondialdehyde (MDA), superoxide dismutase (SOD) , neuron specific enolase (NSE) and myelin basic protein (MBP) in neonates with hypoxic ischemic encephalopathy (HIE).Methods Ninety neonates with HIE were randomly divided into the HBO group ( n=45 ) and the conventional treatment group ( n =45 ) . Another 45 normal neonates borns at the same time were chosen as the control group .Serum levels of MDA , SOD, NSE and MBP were detected with enzyme immunoassay and chemical colorimetric assay , both before and after treatment.Changes in the scores of NBNA were evaluated both before and after treatment , and the relationship between NBNA and the above data was analyzed .Results Before HBO therapy , the MDA, NSE and MBP levels in the neonates with HIE were obviously higher than those of the control group ( P<0.05) [(4.48 ±0.94)nmol/L、(10.02 ±3.10)μg/L、(4.58 ±2.17)μg/L], and the level of SOD was noticeably lower that of the control group (P<0.05)[(137.65 ±34.54)μU/L].Following HBO therapy, the levels of SOD for the HBO group and the routine treatment group were increased , while the levels of MDA , NSE and MBP were decreased , and the scores of NBNA were elevated .Statistical significance could be noted , when compared with those before treatment (P<0.05).More significant changes in the above parameters could be noted in the moderate and severe patients of the HBO group , and statistical significance could be found , when compared with those of the routine treatment group (P<0.01).Results showed that the levels of MBP and NBNA were well correlated .Conclusions HBO therapy could improve the antioxidant capacity of the neonates with HIE and decrease lesion induced by brain tissue lipid peroxidation .Combined detection of MDA , SOD, NSE and MBP was beneficial to the assessment and judgment of the patients'conditions .
