CAJ | 학술논문

目的：探讨微小核糖核苷酸（miRNA）在急性心肌梗死（AMI）早期诊断中的价值。方法：将发病时间＜3h的胸痛患者60例根据其诊断结果分为AMI组（n＝30）和非AMI组（n＝30），另选择正常对照组（n＝30），在入院即刻测定miR－208a、肌钙蛋白、肌红蛋白、磷酸肌酸心肌同工酶（CK－MB），比较各组生化标记物的变化。在AMI组动态监测miR－208a和肌钙蛋白的波动情况；以实时荧光定量聚合酶链反应测定miR－208a。结果：入院即刻AMI组miR－208a明显高于对照组（P＜0．05），但肌钙蛋白和CK－MB与对照组无明显差异；发病1h时的miR－208a显著高于对照组（P＜0．05），肌钙蛋白浓度尚处于正常范围内。 miR－208a的峰值出现于发病12h，峰值明显早于肌钙蛋白。结论：miR－208a在早期AMI诊断中具备高特异性和高敏感性。
목적：탐토미소핵당핵감산（miRNA）재급성심기경사（AMI）조기진단중적개치。방법：장발병시간＜3h적흉통환자60례근거기진단결과분위AMI조（n＝30）화비AMI조（n＝30），령선택정상대조조（n＝30），재입원즉각측정miR－208a、기개단백、기홍단백、린산기산심기동공매（CK－MB），비교각조생화표기물적변화。재AMI조동태감측miR－208a화기개단백적파동정황；이실시형광정량취합매련반응측정miR－208a。결과：입원즉각AMI조miR－208a명현고우대조조（P＜0．05），단기개단백화CK－MB여대조조무명현차이；발병1h시적miR－208a현저고우대조조（P＜0．05），기개단백농도상처우정상범위내。 miR－208a적봉치출현우발병12h，봉치명현조우기개단백。결론：miR－208a재조기AMI진단중구비고특이성화고민감성。
Objective:To explore the value of miR208a in the early diagnosis of acute myocardial infarction (AMI). Methods:60 Patients with chest discomfort within 3 hours were included, 30 in the AMI group and 30 in the non-AMI group. Another 30 healthy people were also included as the control group. Plasma miR-208a,cardiac troponin I, myoglobin and CK-MB were determined once the patients arrived and then compared to the basic level in the control group. MiR-208a was detected at series times thereafter in the AMI group. In the study,miR-208a was determined by quantitative RT-PCR. Results:MiR-208a was higher in the AMI group than the control group with the first plasma sample(P<0.05). At the first hour after the onset of symptoms miR-208a elevated significantly (P<0.05), while cardiac troponin I was normal. The peak level of miR-208a appeared within 12 hours. Conclusions:It is confirmed that the novel biomarker miR-208a had great specificity and sensitivity in the early diagnosis of AMI.