CAJ | 학술논문

目的：研究电针对局灶性脑缺血大鼠神经行为学及缺血侧皮质JAK2 mRNA表达的影响。方法按区组随机化法将250只SD大鼠分为假手术组、模型组、电针组、AG490组、电针＋AG490组，根据不同缺血时间段又分为2 h、1、3、7和21 d五个小组。采用热凝闭大脑中动脉建立局灶性脑缺血模型，电针取“百会”、“大椎”穴，AG490采用侧脑室注射。通过原位杂交的方法检测缺血侧皮质JAK2 mRNA表达变化。结果在假手术组大鼠脑组织中未发现明显JAK2 mRNA表达；模型组JAK2 mRNA同时间段与假手术组比较表达量均增多（P＜0．01），其中以1 d组表达量增高最为显著；电针治疗与AG490干预可降低JAK2 mRNA表达（P＜0．05），以1 d、3 d较为显著。 JAK2 mRNA表达变化上与脑缺血大鼠的神经行为学评分改变基本呈一致性。结论脑缺血后JAK2的超量表达及其活化，可能为脑损伤加重的机制之一。脑缺血后电针治疗可下调JAK2的表达水平，降低其磷酸化活性，阻断JAK2诱导的信号转导通路的异常激活。
목적：연구전침대국조성뇌결혈대서신경행위학급결혈측피질JAK2 mRNA표체적영향。방법안구조수궤화법장250지SD대서분위가수술조、모형조、전침조、AG490조、전침＋AG490조，근거불동결혈시간단우분위2 h、1、3、7화21 d오개소조。채용열응폐대뇌중동맥건립국조성뇌결혈모형，전침취“백회”、“대추”혈，AG490채용측뇌실주사。통과원위잡교적방법검측결혈측피질JAK2 mRNA표체변화。결과재가수술조대서뇌조직중미발현명현JAK2 mRNA표체；모형조JAK2 mRNA동시간단여가수술조비교표체량균증다（P＜0．01），기중이1 d조표체량증고최위현저；전침치료여AG490간예가강저JAK2 mRNA표체（P＜0．05），이1 d、3 d교위현저。 JAK2 mRNA표체변화상여뇌결혈대서적신경행위학평분개변기본정일치성。결론뇌결혈후JAK2적초량표체급기활화，가능위뇌손상가중적궤제지일。뇌결혈후전침치료가하조JAK2적표체수평，강저기린산화활성，조단JAK2유도적신호전도통로적이상격활。
Objective To explore the electro-acupuncture effect on neuroethology and the expression of tyrosine kinase-JAK 2 of is-chemic cortex in rats with the focal cerebral ischemia .Methods The 250 SD rats were randomly divided into sham , model, electroa acu-puncture ( EA) , AG490 and EA+AG490 groups.The model of focal cerebral ischemia was established by the heat-coagulation induced the occlusion of the middle cerebral artery.The electro-acupuncture were applied on Baihui (GV20) and Dazhui (GV14), and AG490 were ap-plied by intracerebroventricular infusion .The expression of JAK2 mRNA in the ischemic cortex was observed by in situ hybridization .Results There was no obvious expression of JAK 2 mRNA found in sham group.In the model group, the expression of JAK2 mRNA was increased (P<0.01).After 1d of cerebral ischemia, the expression of JAK2 mRNA had reached the peak.Electro-acupuncture treatment and AG490 intervention could down-regulate the expression of JAK 2 mRNA ( P<0.05 ) , especially in 1 d and 3 d groups.After cerebral ischemia , the expression of JAK2 mRNA was consistent with the neurological deficits score and cerebral pathomorphological changes .Conclusions After cerebral ischemia, the excessive expression of JAK2, and the JAK2 phosphorylation would be one of mechanisms which the brain injury gets worse.The therapy of electro-acupuncture could reduce the expression of JAK 2, and inhibit JAK2 phosphorylated activation , so as to block the abnormal activation of signal transduction pathway which induced by JAK 2.