CAJ | 학술논문

目的：探讨芹黄素对快速老化SAMP8小鼠学习记忆能力及突触囊泡蛋白（Syn）及生长相关蛋白43（GAP43） mRNA及蛋白表达的影响。方法6月龄SAMP8小鼠随机分为模型组、芹黄素10、20、40 mg／kg剂量组，同龄SAMP1小鼠作为对照组，用Morris水迷宫训练测试其空间学习能力和记忆能力；用Western 印迹及RT－PCR方法测定海马区Syn及GAP43蛋白及mRNA表达。结果芹黄素各剂量组的平均逃避潜伏期均缩短，穿越平台区次数增加，在目标象限的停留时间延长（与SAMP8模型组相比，P＜0．05），海马区Syn、GAP43蛋白及mRNA表达均较模型组增加（P＜0．05）。结论芹黄素可能通过促进海马神经突触Syn及GAP43的表达，提高突触修复及再生能力，改善小鼠的学习记忆。
목적：탐토근황소대쾌속노화SAMP8소서학습기억능력급돌촉낭포단백（Syn）급생장상관단백43（GAP43） mRNA급단백표체적영향。방법6월령SAMP8소서수궤분위모형조、근황소10、20、40 mg／kg제량조，동령SAMP1소서작위대조조，용Morris수미궁훈련측시기공간학습능력화기억능력；용Western 인적급RT－PCR방법측정해마구Syn급GAP43단백급mRNA표체。결과근황소각제량조적평균도피잠복기균축단，천월평태구차수증가，재목표상한적정류시간연장（여SAMP8모형조상비，P＜0．05），해마구Syn、GAP43단백급mRNA표체균교모형조증가（P＜0．05）。결론근황소가능통과촉진해마신경돌촉Syn급GAP43적표체，제고돌촉수복급재생능력，개선소서적학습기억。
Objective To explore the effect of apigenin on learning and memory and the expressions of mRNA and protein of Synato -physin ( Syn) and growth associated protein 43 ( GAP43 ) in senescence-accelerated mouse prone 8 ( SAMP8 ) mice.Methods The 6-month-old SAMP8 mice were randomly divided into model,apigenin 10,20,40 mg/kg administration groups,the same age of SAMP1 mice were treated as control.The spatial learning and memory capacity of mouse was determined by Morris water maze training test .The protein and mRNA expressions of Syn and GAP 43 in the hippocampus of the mice were determined by Western blot and RT-PCR method.Results The average escape latency was decreased ,the residence time in the target quadrant ,and the times of passing through platform were increased in apigenin treated group compared with model group (P<0.05).The protein and mRNA expressions of Syn and GAP43 in hippocampus were increased in apigenin treated group compared with model group .Conclusions Apigenin could enhance the learning and memory capability of the SAMP8 mice,which could be due to the apigenin improving synaptic repair and regeneration of hippocampal synapses by promoting the expressions of Syn and GAP 43.