中国药物评价
中國藥物評價
중국약물평개
Chinese Journal of Drug Evaluation
2015年
3期
129-131
,共3页
孙鸽%李炜宾%岳娟%尹晶晶%安全
孫鴿%李煒賓%嶽娟%尹晶晶%安全
손합%리위빈%악연%윤정정%안전
被动皮肤过敏性试验%阳性对照%佐剂%蓝斑
被動皮膚過敏性試驗%暘性對照%佐劑%藍斑
피동피부과민성시험%양성대조%좌제%람반
Passive cutaneous anaphylaxis test%Positive control%Adjuvant%Blue spot
目的：建立被动皮肤过敏性试验中准确且具可重复的阳性对照。方法：30只SD雄性大鼠随机分为1个阴性对照组（氯化钠注射液＋弗氏不完全佐剂）和4个阳性处理组（阳性1组：卵白蛋白，阳性2组：卵白蛋白＋百白破疫苗，阳性3组：卵白蛋白＋弗氏不完全佐剂，阳性4组：卵白蛋白＋弗氏完全佐剂及弗氏不完全佐剂），分别致敏5次制备抗血清，通过皮内注射被动致敏、48 h后激发，以皮内蓝斑有无及直径判定结果。结果：阴性对照组和阳性1组、2组均未见蓝斑，阳性3组、4组出现蓝斑，其1∶2和1∶8注射点蓝斑直径均大于5 mm。结论：被动皮肤过敏性试验中，致敏时结合使用弗氏佐剂可使阳性率达到100％，以保证试验的准确性和可重复性。
目的：建立被動皮膚過敏性試驗中準確且具可重複的暘性對照。方法：30隻SD雄性大鼠隨機分為1箇陰性對照組（氯化鈉註射液＋弗氏不完全佐劑）和4箇暘性處理組（暘性1組：卵白蛋白，暘性2組：卵白蛋白＋百白破疫苗，暘性3組：卵白蛋白＋弗氏不完全佐劑，暘性4組：卵白蛋白＋弗氏完全佐劑及弗氏不完全佐劑），分彆緻敏5次製備抗血清，通過皮內註射被動緻敏、48 h後激髮，以皮內藍斑有無及直徑判定結果。結果：陰性對照組和暘性1組、2組均未見藍斑，暘性3組、4組齣現藍斑，其1∶2和1∶8註射點藍斑直徑均大于5 mm。結論：被動皮膚過敏性試驗中，緻敏時結閤使用弗氏佐劑可使暘性率達到100％，以保證試驗的準確性和可重複性。
목적：건립피동피부과민성시험중준학차구가중복적양성대조。방법：30지SD웅성대서수궤분위1개음성대조조（록화납주사액＋불씨불완전좌제）화4개양성처리조（양성1조：란백단백，양성2조：란백단백＋백백파역묘，양성3조：란백단백＋불씨불완전좌제，양성4조：란백단백＋불씨완전좌제급불씨불완전좌제），분별치민5차제비항혈청，통과피내주사피동치민、48 h후격발，이피내람반유무급직경판정결과。결과：음성대조조화양성1조、2조균미견람반，양성3조、4조출현람반，기1∶2화1∶8주사점람반직경균대우5 mm。결론：피동피부과민성시험중，치민시결합사용불씨좌제가사양성솔체도100％，이보증시험적준학성화가중복성。
Objective: To establish an accurate and repeatable method for positive control of passive cutaneous anaphylaxis(PCA) test.Methods:30 SD rats were randomly divided into 1 negative control group( sodium chloride injection plus Freund′s adjuvant incom-plete) and 4 positive treatment groups(the positive 1 group:ovalbumin, the positive 2 group:ovalbumin plus diphtheria-pertussis-tetanus vaccine, the positive 3 group: ovalbumin plus Freund′s adjuvant incomplete, the positive 4 group: ovalbumin plus Freund′s adjuvant complete and Freund′s adjuvant incomplete ) were set in the experiment.Firstly, antiserum was prepared with 5 subcutaneous injections.Secondly, passive sensitization was conducted by intradermal injection of antiserum.After 48h, the immuno-stimulation was fol-lowed by intravenous injection of equal amount of antigen.Finally,the result was evaluated by inspecting the amount and diameter of intra-dermal blue spots.Results:The blue spot was not found in the negative control group, the positive 1 group and the positive 2 group.While both the positive 3 group and the positive 4 group produced intradermal blue spots.The average diameter of blue spots of the 1∶2 and 1∶8 antiserum dilution injection was larger than 5 mm.Conclusion:In PCA test, by using different sensitive treatment, we found that sensi-tization with Freund′s adjuvant could carry out 100%positive results.It is an accurate and reliable method for establishment of positive control in PCA test.
