海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2015年
7期
66-68
,共3页
宫颈液基细胞学检查(TCT)%宫颈细胞DNA倍体定量分析%宫颈癌%筛查
宮頸液基細胞學檢查(TCT)%宮頸細胞DNA倍體定量分析%宮頸癌%篩查
궁경액기세포학검사(TCT)%궁경세포DNA배체정량분석%궁경암%사사
Liquid-based cervical cytology (TCT)%Quantitative analysis of cervical cellular DNA ploidy%Cervical Cancer%Screening
目的：评价宫颈液基细胞学检查（ TCT）联合宫颈细胞DNA倍体定量分析在宫颈癌筛查中的意义。方法对2013年10月到2014年12月来我院就诊的2250名女性采用TCT联合宫颈细胞DNA倍体分析的筛查，宫颈液基细胞学制片2张，一张行巴氏染色做常规细胞学检查，另一张用硫堇－Feulgen染色后，采用DNA定量分析系统对细胞核DNA含量进行定量分析。以病理检查作为金标准。结果①2250例宫颈脱落细胞样本中，TCT阳性137例，阳性率6．09％；DNA倍体分析阳性164例，阳性率7．29％；TCT联合DNA倍体分析阳性192例，阳性率8．53％；②TCT阳性者进行组织活检，符合率54．01％（74／137）；DNA倍体分析阳性者进行组织活检，符合率48．78％（80／164）；TCT联合DNA倍体分析检测阳性，符合率70．31％（135／192）。结论宫颈液基细胞学检查联合DNA倍体定量分析，能提高诊断敏感性和符合率。
目的：評價宮頸液基細胞學檢查（ TCT）聯閤宮頸細胞DNA倍體定量分析在宮頸癌篩查中的意義。方法對2013年10月到2014年12月來我院就診的2250名女性採用TCT聯閤宮頸細胞DNA倍體分析的篩查，宮頸液基細胞學製片2張，一張行巴氏染色做常規細胞學檢查，另一張用硫堇－Feulgen染色後，採用DNA定量分析繫統對細胞覈DNA含量進行定量分析。以病理檢查作為金標準。結果①2250例宮頸脫落細胞樣本中，TCT暘性137例，暘性率6．09％；DNA倍體分析暘性164例，暘性率7．29％；TCT聯閤DNA倍體分析暘性192例，暘性率8．53％；②TCT暘性者進行組織活檢，符閤率54．01％（74／137）；DNA倍體分析暘性者進行組織活檢，符閤率48．78％（80／164）；TCT聯閤DNA倍體分析檢測暘性，符閤率70．31％（135／192）。結論宮頸液基細胞學檢查聯閤DNA倍體定量分析，能提高診斷敏感性和符閤率。
목적：평개궁경액기세포학검사（ TCT）연합궁경세포DNA배체정량분석재궁경암사사중적의의。방법대2013년10월도2014년12월래아원취진적2250명녀성채용TCT연합궁경세포DNA배체분석적사사，궁경액기세포학제편2장，일장행파씨염색주상규세포학검사，령일장용류근－Feulgen염색후，채용DNA정량분석계통대세포핵DNA함량진행정량분석。이병리검사작위금표준。결과①2250례궁경탈락세포양본중，TCT양성137례，양성솔6．09％；DNA배체분석양성164례，양성솔7．29％；TCT연합DNA배체분석양성192례，양성솔8．53％；②TCT양성자진행조직활검，부합솔54．01％（74／137）；DNA배체분석양성자진행조직활검，부합솔48．78％（80／164）；TCT연합DNA배체분석검측양성，부합솔70．31％（135／192）。결론궁경액기세포학검사연합DNA배체정량분석，능제고진단민감성화부합솔。
OBJECTIVE To evaluate the significance of liquid-based cervical cytology combined with quantita-tive analysis of cervical cellular DNA ploidy in cervical cancer screening .METHODS Screening of cervical cancer was performed by liquid-based cytology combined with quantitative analysis of cellular DNA ploidy in 2250 women who were in our hospital for visit from October 2013 to December 2014.The samples were taken by cervix brush and prepared for 2 slides:one was stained by pap staining and read by cytologist .The other was stained with Thionin-FeulgenstainingandtestbyDNAimagescytometry.RESULTS ①TheTCTpositiveresultswereobservedin137 case ( 6.09%) .The DNA positive results were observed in 164 case ( 7.29%) .The TCT +DNA positive results were observed in 192 case(8.53%).②By colposcopy biopsy ,the positive coincidence rate of the TCT positive was 54.01%(74/137),the positive coincidence of The DNA positive was 48.78%(80/164).the positive conincidence of the TCT+DNA positive was 70.31%(135/192).CONCLUSION The liquid-based cervical cytology combined with quantitative analysis of cervical cellular DNA ploidy could improve the sensitivity and coincidence rate .
