国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
14期
1078-1081
,共4页
苏强%王培银%马妮娜%李琴%俞静%曹邦伟
囌彊%王培銀%馬妮娜%李琴%俞靜%曹邦偉
소강%왕배은%마니나%리금%유정%조방위
烟酰胺磷酸核糖转移酶抑制剂%FK866%厄洛替尼%非小细胞肺癌%凋亡
煙酰胺燐痠覈糖轉移酶抑製劑%FK866%阨洛替尼%非小細胞肺癌%凋亡
연선알린산핵당전이매억제제%FK866%액락체니%비소세포폐암%조망
Nicotinamide phosphoribosyltransferase inhibitor%FK866%Erlotinib%Non-small cell lung cancer%Apoptosis
目的 探讨FK866对酪氨酸激酶抑制剂耐药非小细胞肺癌A549细胞凋亡的影响及其可能的机制.方法 FK866和厄洛替尼单药以及两者联合作用A549细胞,72 h后,MTT法检测细胞的增殖并计算细胞活度,用流式细胞学检测A549细胞凋亡率.结果 厄洛替尼联合FK866比单药组降低了A549的细胞活度,流式细胞学显示二者联合比单药更能促进A549细胞的凋亡.结论 初步得出FK866联合组比单药组更能抑制A549细胞的增殖,促进A549细胞的凋亡.
目的 探討FK866對酪氨痠激酶抑製劑耐藥非小細胞肺癌A549細胞凋亡的影響及其可能的機製.方法 FK866和阨洛替尼單藥以及兩者聯閤作用A549細胞,72 h後,MTT法檢測細胞的增殖併計算細胞活度,用流式細胞學檢測A549細胞凋亡率.結果 阨洛替尼聯閤FK866比單藥組降低瞭A549的細胞活度,流式細胞學顯示二者聯閤比單藥更能促進A549細胞的凋亡.結論 初步得齣FK866聯閤組比單藥組更能抑製A549細胞的增殖,促進A549細胞的凋亡.
목적 탐토FK866대락안산격매억제제내약비소세포폐암A549세포조망적영향급기가능적궤제.방법 FK866화액락체니단약이급량자연합작용A549세포,72 h후,MTT법검측세포적증식병계산세포활도,용류식세포학검측A549세포조망솔.결과 액락체니연합FK866비단약조강저료A549적세포활도,류식세포학현시이자연합비단약경능촉진A549세포적조망.결론 초보득출FK866연합조비단약조경능억제A549세포적증식,촉진A549세포적조망.
Objective To investigate nicotinamide phosphoribosyltransferase inhibitor,FK866,enhance erlotinib-induced apoptosis in epidermal growth factor receptor-tyrosine kinase inhibitor-resistant non-small cell lung cancer A549 cells.Methods After FK866 or erlotinib,combined treatment in A549 cells for 72 hours,the cell proliferation was detected and the cell activity was calculated by MTT,the apoptosis rate of A549 cells was detected by flow cytometry.Results This combined treatment significantly reduced the activity of A549 cells,and enhanced apoptosis of A549 cells compared with FK866 or erlotinib single treatment.Conclusions It is indicated that FK866 can enhance erlotinib-induced apoptosis in epidermal growth factor receptor-tyrosine kinase inhibitor-resistant non-small cell lung cancer A549 cells.
