CAJ | 학술논문

目的：建立高效液相色谱法同时测定依折麦布阿托伐他汀钙片中依折麦布和阿托伐他汀的含量。方法色谱柱为 Agilent ZORBAX RX－C8（250mm ×4．6mm，5μm），流动相为0．05mol? L－1醋酸铵缓冲液（冰醋酸调节 pH 至5．0±0．1）－四氢呋喃－乙腈（55∶12∶33，V／V／V），流速为1．2mL? min －1，检测波长为244nm，柱温为35℃，进样量为20μL。结果在所建立的色谱条件下，阿托伐他汀钙主峰和依折麦布主峰分离良好。结论该方法操作简单，灵敏度高，准确可靠，可用于依折麦布阿托伐他汀片含量的测定。
목적：건립고효액상색보법동시측정의절맥포아탁벌타정개편중의절맥포화아탁벌타정적함량。방법색보주위 Agilent ZORBAX RX－C8（250mm ×4．6mm，5μm），류동상위0．05mol? L－1작산안완충액（빙작산조절 pH 지5．0±0．1）－사경부남－을정（55∶12∶33，V／V／V），류속위1．2mL? min －1，검측파장위244nm，주온위35℃，진양량위20μL。결과재소건립적색보조건하，아탁벌타정개주봉화의절맥포주봉분리량호。결론해방법조작간단，령민도고，준학가고，가용우의절맥포아탁벌타정편함량적측정。
OBJECTIVE To establish a HPLC method for determination of ezetimibe and atorvastatin in ezetimi-be and atorvastatin calcium tablets.METHODS The reported method was developed on a Agilent ZORBAX RX-C8 (250mm ×4.6mm,5μm).Mobile phase was comprised of 0.05mol? L-1 ammonium acetate solution(adjust the pH to 5.0 ±0.1 by using glacial acetic acid ),tetrahydrofuran,and acetonitrile in the ratio of (55∶12∶33,V/V/V);A flow rate was set at 1.2 mL? min -1 with UV detector of 244 nm and column temperature of 35℃,injection volume was set at 20μL.RESULT Under this chromatographic system ,the separation between atorvastatin and ezetimibe main peak was good.CONCLUSION The method is convenient ,sensitive,and accurate.It can be used for the determi-nation of ezetimibe and atorvastatin calcium tablets .