CAJ | 학술논문

目的：建立八珍益母胶囊中槲皮素的HPLC的测定方法，为制定该制剂质量标准中定量测定方法及限度提供依据。方法色谱柱：Sun－fire－C18（4．6mm ×150mm，5μm）；流动相：以甲醇－0．4％磷酸溶液（50∶50）；流速：1．0mL? min －1，检测波长：360nm，柱温：25℃，进样量：10μL。结果槲皮素的线性范围分别为34．35～429．43μg，平均加样回收率（n＝6）分别为99．26％，RSD分别为0．26％。结论该方法简单、快速、结果准确，重现性好，为八珍益母胶囊中槲皮素的测定提供可靠的方法。
목적：건립팔진익모효낭중곡피소적HPLC적측정방법，위제정해제제질량표준중정량측정방법급한도제공의거。방법색보주：Sun－fire－C18（4．6mm ×150mm，5μm）；류동상：이갑순－0．4％린산용액（50∶50）；류속：1．0mL? min －1，검측파장：360nm，주온：25℃，진양량：10μL。결과곡피소적선성범위분별위34．35～429．43μg，평균가양회수솔（n＝6）분별위99．26％，RSD분별위0．26％。결론해방법간단、쾌속、결과준학，중현성호，위팔진익모효낭중곡피소적측정제공가고적방법。
OBJECTIVE To establish a method for determing quercetin in Bazhenyimu capsules by HPLC.METHODS The HPLC method was carried out on Agilent C 18 column(4.6mm ×150mm,5μm),The mo-bile phase was methanol-0.5%phosphate(50∶50);The flow rate was 1.0mL? min-1,The detection wavelength was at 360nm,The column temperature was set at 25℃,and 10μL injection volumn. RESULTS There was a good lin-ear relationship when quercetin were in the range of 34.35~429.43μg.The average recoveries were 99.26%, re-spective RSD were 0.26%.CONCLUSION The method is simple in operation ,high in sensitivity and good in re-producibility ,and can be used for the determination the quercetin in Bazhenyimu capsules by HPLC .