CAJ | 학술논문

目的：建立HPLC法测定复方益节健片中淫羊藿苷的含量。方法采用高效液相色谱法，色谱柱：Diamonsil C18柱（4．6mm ×250mm，5μm），流动相：水－乙腈（70∶30）；流速：1mL? min －1；柱温：30℃，检测波长：270nm；进样量：10μL。结果淫羊藿苷的质量浓度在10～50μg?mL －1（r＝0．9997，n＝5）范围内与其峰面积呈良好的线性关系。精密度、稳定性、重复性试验的 RSD＜3％；平均加样回收率为99．28％，RSD＝1．32％（ n＝6）。结论该方法简便，准确，重复性好，可用于复方益节健片中淫羊藿苷的含量测定。
목적：건립HPLC법측정복방익절건편중음양곽감적함량。방법채용고효액상색보법，색보주：Diamonsil C18주（4．6mm ×250mm，5μm），류동상：수－을정（70∶30）；류속：1mL? min －1；주온：30℃，검측파장：270nm；진양량：10μL。결과음양곽감적질량농도재10～50μg?mL －1（r＝0．9997，n＝5）범위내여기봉면적정량호적선성관계。정밀도、은정성、중복성시험적 RSD＜3％；평균가양회수솔위99．28％，RSD＝1．32％（ n＝6）。결론해방법간편，준학，중복성호，가용우복방익절건편중음양곽감적함량측정。
OBJECTIVE To establish an HPLC method for the determination of Icarii in Compound Yijiejian Tablets.METHODS The chromatographic separation was performed on Diamonsil C 18 (4.6mm ×250mm,5μm) analytical column with mobile phase A-acetonitrile ,B-water at a flow rate of 1.0 mL? min-1 .The column temperature was at 30℃;the detection wavelength was set at 270 nm and the sample volume was 10μL.RESULTS The linear range of icariin was 10~50μg(r=0.9997,n=5).The average recovery rate was 99.28%(RSD=1.32%,n=6) .The RSD of precision ,reproducibility and stability tests were lower than 3%.CONCLUSION This method is sim-ple,accurate and has a good repeatability ,which can be used for the determination of Compound Yijiejian Tablets .