海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2015年
7期
51-53
,共3页
铁氰化钾%普鲁士蓝%头孢唑啉钠%分光光度法
鐵氰化鉀%普魯士藍%頭孢唑啉鈉%分光光度法
철청화갑%보로사람%두포서람납%분광광도법
Potassium ferricyanide%Prussian blue%Cefazolin sodium%Spectrophotometry
目的：建立了利用生成普鲁士蓝测定头孢唑啉钠含量的新方法。方法头孢唑啉钠在0．10mol? L－1 NaOH溶液中、100℃水浴加热降解为含巯基化合物。然后加入 FeCl3，巯基化合物能把 Fe3＋还原为 Fe2＋，Fe2＋与 K3〔Fe（CN）6〕生成可溶性普鲁士蓝，通过测定普鲁士蓝在740 nm的吸光度，间接测定头孢唑啉钠的含量。结果头孢唑啉钠含量在0．11～9．60μg? mL －1均具有良好的线性关系，线性回归方程 A ＝0．2041C（μg? mL －1）－0．0227，相关系数R＝0．999。摩尔吸光系数ε740＝9．7×104L／（mol? cm）。结论本法用于测定头孢唑啉钠粉针剂，平均回收率为99．74％，样品测定结果与中国药典法的测定结果基本一致。
目的：建立瞭利用生成普魯士藍測定頭孢唑啉鈉含量的新方法。方法頭孢唑啉鈉在0．10mol? L－1 NaOH溶液中、100℃水浴加熱降解為含巰基化閤物。然後加入 FeCl3，巰基化閤物能把 Fe3＋還原為 Fe2＋，Fe2＋與 K3〔Fe（CN）6〕生成可溶性普魯士藍，通過測定普魯士藍在740 nm的吸光度，間接測定頭孢唑啉鈉的含量。結果頭孢唑啉鈉含量在0．11～9．60μg? mL －1均具有良好的線性關繫，線性迴歸方程 A ＝0．2041C（μg? mL －1）－0．0227，相關繫數R＝0．999。摩爾吸光繫數ε740＝9．7×104L／（mol? cm）。結論本法用于測定頭孢唑啉鈉粉針劑，平均迴收率為99．74％，樣品測定結果與中國藥典法的測定結果基本一緻。
목적：건립료이용생성보로사람측정두포서람납함량적신방법。방법두포서람납재0．10mol? L－1 NaOH용액중、100℃수욕가열강해위함구기화합물。연후가입 FeCl3，구기화합물능파 Fe3＋환원위 Fe2＋，Fe2＋여 K3〔Fe（CN）6〕생성가용성보로사람，통과측정보로사람재740 nm적흡광도，간접측정두포서람납적함량。결과두포서람납함량재0．11～9．60μg? mL －1균구유량호적선성관계，선성회귀방정 A ＝0．2041C（μg? mL －1）－0．0227，상관계수R＝0．999。마이흡광계수ε740＝9．7×104L／（mol? cm）。결론본법용우측정두포서람납분침제，평균회수솔위99．74％，양품측정결과여중국약전법적측정결과기본일치。
OBJECTIVE A new spectrophotometric method has been established to determine cefazolin sodium using the generated Prussian blue .METHOD In the 0.1mol? L-1 NaOH solution,cefazolin sodium can be de-graded to form sulfhydryl group at 100℃water-bath heating.Then,the Fe3+will be reduced to form Fe2+by sulfhydryl group after we adding FeCl3 .And the soluble Prussian blue is produced by the reaction between Fe2+and K3〔Fe( CN) 6〕.By determining the absorbance of Prussian blue in the 740 nm wavelength,the indi-rect determination of cefazolin sodium can be achieved.RESULTS A good linear relationship is obtained when the content of cefazolin sodium range from 0.11μg? mL-1 to 9.60μg? mL-1 .The linear regression equation is A =0.2041 C (μg? mL-1 ) -0.0227 with a correlation coefficient of 0.999 and the apparent molar absorption coefficient is 9.7 ×104L? (mol? cm-1).CONCLUSION This method has been applied to the determination of cefazolin so-dium powder injection with satisfactory results ,The average recovery was 99.74%.The determination results of sam-ple were almost identical to those obtained by Ch .P method.
