中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2015年
2期
135-139,封3-封4
,共6页
王翠翠%刘晓红%曹永成%毕利泉%耿明
王翠翠%劉曉紅%曹永成%畢利泉%耿明
왕취취%류효홍%조영성%필리천%경명
减压病%脊髓损伤%星形细胞%小神经胶质细胞%肿瘤坏死因子
減壓病%脊髓損傷%星形細胞%小神經膠質細胞%腫瘤壞死因子
감압병%척수손상%성형세포%소신경효질세포%종류배사인자
Decompression sickness%Spinal cord injuries%Astrocytes%Microglia%Tumor necrosis factor-alpha
目的 探讨星型胶质细胞与小胶质细胞的活化在家兔减压病脊髓损伤中的作用. 方法 成年健康雄性新西兰家兔21只,按随机数字表法分为对照组、安全减压组和减压病(decompression sickness,DCS)组,每组7只.实验动物置于加压舱内.安全减压组参考我国海军空气潜水减压表减至常压;DCS组5 min匀速加压至0.8 MPa(绝对压),停留60 min后,再5 min匀速减至常压.光镜观察胸腰段脊髓的病理形态学改变;荧光实时定量法测定肿瘤坏死因子-a(tumor necrosis factor-α,TNF-α)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)基因mRNA表达;免疫组织化学方法测定TNF-α、GFAP和钙结合蛋白(ionized calcium binding adapter molecule 1,IBA1)的表达.结果 DCS组脊髓白质内空泡形成,脊髓中TNF-α基因mRNA相对表达量(6.28±1.73)与对照组(1.00±o.14)和安全减压组(1.34±0.42)相比,差异均有统计学意义(P＜0.01);GFAP基因mRNA相对表达量(7.39±2.04)与对照组(1.02±0.26)和安全减压组(1.63±0.90)相比,差异均有统计学意义(P＜0.01).TNF-α蛋白表达量(24.14±2.61)较对照组(6.71±1.25)和安全减压组(8.28±1.11)明显增加,差异均有统计学意义(P＜0.01);GFAP蛋白表达量(18.20±4.38)较对照组(4.30±2.70)和安全减压组(6.20±2.92)明显增加,差异均有统计学意义(P＜0.01);IBA1蛋白表达量(21.53±1.37)较对照组(5.94±0.36)和安全减压组(6.69±0.81)明显增加,差异均有统计学意义(P＜0.01). 结论 星形胶质细胞和小胶质细胞在减压病脊髓损伤的病理生理过程中发挥重要作用,在DCS的早期即可被激活,释放大量的TNF-α,引起过度炎症反应,导致脊髓损伤.
目的 探討星型膠質細胞與小膠質細胞的活化在傢兔減壓病脊髓損傷中的作用. 方法 成年健康雄性新西蘭傢兔21隻,按隨機數字錶法分為對照組、安全減壓組和減壓病(decompression sickness,DCS)組,每組7隻.實驗動物置于加壓艙內.安全減壓組參攷我國海軍空氣潛水減壓錶減至常壓;DCS組5 min勻速加壓至0.8 MPa(絕對壓),停留60 min後,再5 min勻速減至常壓.光鏡觀察胸腰段脊髓的病理形態學改變;熒光實時定量法測定腫瘤壞死因子-a(tumor necrosis factor-α,TNF-α)、膠質纖維痠性蛋白(glial fibrillary acidic protein,GFAP)基因mRNA錶達;免疫組織化學方法測定TNF-α、GFAP和鈣結閤蛋白(ionized calcium binding adapter molecule 1,IBA1)的錶達.結果 DCS組脊髓白質內空泡形成,脊髓中TNF-α基因mRNA相對錶達量(6.28±1.73)與對照組(1.00±o.14)和安全減壓組(1.34±0.42)相比,差異均有統計學意義(P＜0.01);GFAP基因mRNA相對錶達量(7.39±2.04)與對照組(1.02±0.26)和安全減壓組(1.63±0.90)相比,差異均有統計學意義(P＜0.01).TNF-α蛋白錶達量(24.14±2.61)較對照組(6.71±1.25)和安全減壓組(8.28±1.11)明顯增加,差異均有統計學意義(P＜0.01);GFAP蛋白錶達量(18.20±4.38)較對照組(4.30±2.70)和安全減壓組(6.20±2.92)明顯增加,差異均有統計學意義(P＜0.01);IBA1蛋白錶達量(21.53±1.37)較對照組(5.94±0.36)和安全減壓組(6.69±0.81)明顯增加,差異均有統計學意義(P＜0.01). 結論 星形膠質細胞和小膠質細胞在減壓病脊髓損傷的病理生理過程中髮揮重要作用,在DCS的早期即可被激活,釋放大量的TNF-α,引起過度炎癥反應,導緻脊髓損傷.
목적 탐토성형효질세포여소효질세포적활화재가토감압병척수손상중적작용. 방법 성년건강웅성신서란가토21지,안수궤수자표법분위대조조、안전감압조화감압병(decompression sickness,DCS)조,매조7지.실험동물치우가압창내.안전감압조삼고아국해군공기잠수감압표감지상압;DCS조5 min균속가압지0.8 MPa(절대압),정류60 min후,재5 min균속감지상압.광경관찰흉요단척수적병리형태학개변;형광실시정량법측정종류배사인자-a(tumor necrosis factor-α,TNF-α)、효질섬유산성단백(glial fibrillary acidic protein,GFAP)기인mRNA표체;면역조직화학방법측정TNF-α、GFAP화개결합단백(ionized calcium binding adapter molecule 1,IBA1)적표체.결과 DCS조척수백질내공포형성,척수중TNF-α기인mRNA상대표체량(6.28±1.73)여대조조(1.00±o.14)화안전감압조(1.34±0.42)상비,차이균유통계학의의(P＜0.01);GFAP기인mRNA상대표체량(7.39±2.04)여대조조(1.02±0.26)화안전감압조(1.63±0.90)상비,차이균유통계학의의(P＜0.01).TNF-α단백표체량(24.14±2.61)교대조조(6.71±1.25)화안전감압조(8.28±1.11)명현증가,차이균유통계학의의(P＜0.01);GFAP단백표체량(18.20±4.38)교대조조(4.30±2.70)화안전감압조(6.20±2.92)명현증가,차이균유통계학의의(P＜0.01);IBA1단백표체량(21.53±1.37)교대조조(5.94±0.36)화안전감압조(6.69±0.81)명현증가,차이균유통계학의의(P＜0.01). 결론 성형효질세포화소효질세포재감압병척수손상적병리생리과정중발휘중요작용,재DCS적조기즉가피격활,석방대량적TNF-α,인기과도염증반응,도치척수손상.
Objective To investigate the effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness (DCS).Methods Twenty-one healthy adult male New Zealand rabbits were averagely divided into 3 groups according to random number table:control group,safety relief group and DCS group.Experimental animals were placed in the pressure cabin.Safety relief group model referred to the Chinese Navy diving decompression tables when decompression applied.In DCS group,the pressure equably was increased to 0.8 MPa (absolute pressure) within 5 min by compressed air,maintained for 60 min and then equably decompressed to normal pressure within 5 min.The change of pathology morphology in the spinal cord of thoracolumbar vertebra was observed by light microscope.The expression of tumor necrosis factor-a (TNF-a) and Glial Fibrillary Acidic Protein (GFAP) mRNA were measured by real-time polymerase chain reaction (real-time PCR) and the expression of TNF-α,GFAP,ionized calcium binding adapter molecule 1 (IBA1) protein was measured by immunohistochemistry method.Results There were some cavity formations on white matter of spinal cord in DCS group.The relative expression of TNF-α mRNA was higher in DCS (6.28±1.73) than that in control group (1.00±0.14) and in safety relief group (1.34±0.42) (P＜0.01).The relative expression of GFAP mRNA was higher in DCS (7.39±2.04) than in that control group (1.02±0.26) and in safety relief group (1.63±0.90) (P＜ 0.01).The expression of TNF-α protein was significantly higher in DCS group (24.14± 2.61) than that in control group (6.71±1.25) and in safety relief group (8.28 ± 1.11) (P＜0.01).The expression of GFAP protein was also significantly higher in DCS (18.20±4.38) than that in control group (4.30±2.70) and in safety relief group (6.20±2.92) (P＜0.01).The expression of IBA1 protein was also significantly higher in DCS (21.53±1.37) than that in control group (5.94±0.36) and in safety relief group (6.69 ± 0.81) (P＜0.01).Conclusions The astrocyte and microglia may play a key role in pathophysiology of spinal cord injury.They are activated in the early stage of DCS and accompanied with massive release of TNF-α,which causes excessive inflammation reaction and induces the spinal cord injury finally.
