CAJ | 학술논문

目的：正交法优选新疆紫草中紫草素的提取方法。方法以紫草素的含量为指标，用HPLC法测定紫草素的含量，色谱柱：Hedera ODS－2色谱柱（4．6mm ×250mm，5μm）；流动相：甲醇：0．05％甲酸－水（80：20）；流速：1mL? min －1；检测波长：516nm；进样量：20μL；柱温：30℃。考察提取时间、加醇倍量、温度对紫草素的提取的影响。结果紫草素线性范围是2．5～40．0μg? mL －1， r＝0．9999；平均加样回收率为99．96％。紫草中紫草素的最佳提取工艺为：提取4h，10倍量95％乙醇，温度50℃。结论该工艺合理，稳定可行，可为紫草素的提取工艺提供依据。
목적：정교법우선신강자초중자초소적제취방법。방법이자초소적함량위지표，용HPLC법측정자초소적함량，색보주：Hedera ODS－2색보주（4．6mm ×250mm，5μm）；류동상：갑순：0．05％갑산－수（80：20）；류속：1mL? min －1；검측파장：516nm；진양량：20μL；주온：30℃。고찰제취시간、가순배량、온도대자초소적제취적영향。결과자초소선성범위시2．5～40．0μg? mL －1， r＝0．9999；평균가양회수솔위99．96％。자초중자초소적최가제취공예위：제취4h，10배량95％을순，온도50℃。결론해공예합리，은정가행，가위자초소적제취공예제공의거。
OBJECTIVE To optimize the extraction of alkannin from Arnebia euchroma ( Royel) Johnst by or-thogonal design.METHODS Referring to the content of alkannin ,HPLC was employed for the content determina-tion with Hedera ODS-2(4.6mm ×250mm,5μm)and the mobile phase of methanol-0.05%formic acid solution (80∶20 ) at detection wavelength of 516 nm.The column temperature was 30℃and flow rate was 1.0 mL? min -1 .Factors of temperature、times amount of 95%ethanol and extraction time were studied .RESULTS Alkannin had linear in the range between 2.5~40.0μg? mL-1 , r=0.9999;The recovery was 99.96%.The optimal process of extraction was as follows:the extraction time was 4h,adding 10 times 95% ethanol,at 50℃.CONCLUSION The extract process is reasonable and practicable ,which can be used as basis for the extraction process of alkannin .