CAJ | 학술논문

目的探讨持续性正加速度(+Gz)暴露对大鼠牙龈组织的影响以及丹参多酚酸的保护作用. 方法采用随机区组法将27只雄性SD大鼠分为阴性对照组、+5Gz阳性对照组、+5Gz药物组(低、中、高剂量)、+10 Gz阳性对照组、+10 Gz药物组(低、中、高剂量),共9组,每组3只.除阴性对照组外,其余各组动物分别在+5Gz和+10 Gz的条件下各离心30 s,每天重复5次,间隔以+1Gz持续60 s,每周4d,共3周.实验期间给药组于每次离心前30 min按剂量腹腔注射丹参多酚酸溶液(低、中、高给药剂量分别为2、6、18 mg/kg),3个对照组均注射等体积生理盐水.观察大鼠行为活动的变化.光镜观察各组大鼠牙龈组织形态学变化,并采用PCR法测定牙龈组织中热休克蛋白70(heat shock protein 70,HSP70) mRNA相对含量. 结果持续性正加速度对大鼠的行为活动有一定程度的影响,采用丹参多酚酸干预后,大鼠的活动程度有逐渐恢复的趋势.各组大鼠牙龈组织呈红色,有光泽,质地坚韧,未见明显肿胀和出血.对照组牙龈组织上皮基本完整;+5 Gz阳性对照组和药物组的牙龈组织上皮过度角化,粒层明显;+10 Gz阳性对照组和药物组牙龈组织上皮角化程度更高,粒层更明显.随着正加速度G值的增加,大鼠牙龈组织HSP70 mRNA相对表达量亦增加(F=169.22、650.69,P＜0.01),随着丹参多酚酸给药浓度的增加,HSP70 mRNA的相对表达量受到明显抑制(F=68.73,P＜0.01). 结论 HSP70 mRNA相对表达量与+Gz值的增加呈正相关,+Gz值越大对大鼠牙龈组织的损伤也越大;丹参多酚酸对+Gz暴露下的牙龈组织损伤具有一定的保护作用.
목적 탐토지속성정가속도(+Gz)폭로대대서아간조직적영향이급단삼다분산적보호작용. 방법 채용수궤구조법장27지웅성SD대서분위음성대조조、+5Gz양성대조조、+5Gz약물조(저、중、고제량)、+10 Gz양성대조조、+10 Gz약물조(저、중、고제량),공9조,매조3지.제음성대조조외,기여각조동물분별재+5Gz화+10 Gz적조건하각리심30 s,매천중복5차,간격이+1Gz지속60 s,매주4d,공3주.실험기간급약조우매차리심전30 min안제량복강주사단삼다분산용액(저、중、고급약제량분별위2、6、18 mg/kg),3개대조조균주사등체적생리염수.관찰대서행위활동적변화.광경관찰각조대서아간조직형태학변화,병채용PCR법측정아간조직중열휴극단백70(heat shock protein 70,HSP70) mRNA상대함량. 결과 지속성정가속도대대서적행위활동유일정정도적영향,채용단삼다분산간예후,대서적활동정도유축점회복적추세.각조대서아간조직정홍색,유광택,질지견인,미견명현종창화출혈.대조조아간조직상피기본완정;+5 Gz양성대조조화약물조적아간조직상피과도각화,립층명현;+10 Gz양성대조조화약물조아간조직상피각화정도경고,립층경명현.수착정가속도G치적증가,대서아간조직HSP70 mRNA상대표체량역증가(F=169.22、650.69,P＜0.01),수착단삼다분산급약농도적증가,HSP70 mRNA적상대표체량수도명현억제(F=68.73,P＜0.01). 결론 HSP70 mRNA상대표체량여+Gz치적증가정정상관,+Gz치월대대대서아간조직적손상야월대;단삼다분산대+Gz폭로하적아간조직손상구유일정적보호작용.
Objective To investigate changes in gingival tissues of the rats in sustained + Gz acceleration exposure and the protective effects of salvia miltiorrhiza polyphenols acid.Methods Twenty-seven male SD rats were randomly divided into negative control,+ 5 Gz positive control,+5 Gz dose group (low,moderate,and high dose),+10 Gz positive control and +10 Gz dose group (low,moderate,and high dose) groups.Except negative control group,other groups were exposed to +5 Gz or +10 Gz for 30 s with 60 s intervals,5 times a day,4 days a week for 3 consecutive weeks.Salvia miltiorrhiza polyphenols acid was injected into rat's abdomen with the different doses (2,6,18 mg/kg) 30 min before each exposure.Three control groups were injected equivalent amount of 0.9％ sodium chloride solution.The rat's behavior and gingival tissues changes were observed after the experiments.Real time PCR was applied to determine the relative expression of heat shock protein 70 (HSP70) mRNA in gingival tissues.Results Sustained high +Gz exposure influenced the active degree of model rats in a certain range and salvia miltiorrhiza polyphenols acid helped the rats turning to the activity recovery.The gingival tissues of every groups showed red,sheen and with firm and tenacious character,and no obvious plumping and bleeding were observed.The epithelium of gingival tissues of negative control group kept its integrity,but the epithelium of +5 Gz positive control and dose groups showed significant keratinization and granular layer.Situations were more serious for +10 Gz positive and +10 Gz dose groups.The relative expression of HSP70 mRNA was increased with the growing of +Gz level (F=169.22,650.69,P＜0.01),but showed significant restraint with the augment of salvia miltiorrhiza polyphenols acid dose (F=68.73,P＜0.01).Conclusions The relative expression of HSP70 mRNA in gingival tissues of rats is positively correlated to +Gz level.The higher +Gz level the more harmful to the gingival tissues of rats.Salvia miltiorrhiza polyphenols acid has certain protective effect on the gingival tissues of rats.