中国麻风皮肤病杂志
中國痳風皮膚病雜誌
중국마풍피부병잡지
CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
2015年
8期
462-466
,共5页
IL-8%表皮生长因子%黑素瘤细胞%A375
IL-8%錶皮生長因子%黑素瘤細胞%A375
IL-8%표피생장인자%흑소류세포%A375
IL-8%EGF%melanoma cell%A375
目的：：明确IL－8与表皮生长因子( EGF)对黑素瘤细胞增殖的影响。方法：将黑素瘤细胞A375分为9组：EGF组；EGF＋AG1478组；AG1478组；IL－8组；IL－8I组；IL－8＋IL－8I组；EGF＋ IL－8组；EGF＋IL－8＋AG1478＋IL－8I组及对照组。采用MTT法检测各组细胞增殖情况；免疫印迹法检测EG-FR、蛋白激酶B、丝裂原活化蛋白激酶、磷酸化蛋白激酶B、磷酸化丝裂原活化蛋白激酶、磷酸化表皮生长因子受体的表达；实时荧光定量聚合酶链式反应检测EGFR与IL－8R mRNA水平的表达。结果：EGF组黑素瘤细胞增殖、上述蛋白的表达和mRNA水平显著高于对照组、EGF＋AG1478组和AG1478组。IL－8组显著高于对照组、IL－8I组和IL－8＋IL－8I组(均P<0.05)，EGF＋AG1478组显著高于EGF组和IL－8组( P<0.05)。结论： IL－8与EGF均能促进人黑素瘤细胞的增殖，它们之间可能具有相互协同作用，这种协同作用可能与AKT和MAPK等共同通路作用有关。
目的：：明確IL－8與錶皮生長因子( EGF)對黑素瘤細胞增殖的影響。方法：將黑素瘤細胞A375分為9組：EGF組；EGF＋AG1478組；AG1478組；IL－8組；IL－8I組；IL－8＋IL－8I組；EGF＋ IL－8組；EGF＋IL－8＋AG1478＋IL－8I組及對照組。採用MTT法檢測各組細胞增殖情況；免疫印跡法檢測EG-FR、蛋白激酶B、絲裂原活化蛋白激酶、燐痠化蛋白激酶B、燐痠化絲裂原活化蛋白激酶、燐痠化錶皮生長因子受體的錶達；實時熒光定量聚閤酶鏈式反應檢測EGFR與IL－8R mRNA水平的錶達。結果：EGF組黑素瘤細胞增殖、上述蛋白的錶達和mRNA水平顯著高于對照組、EGF＋AG1478組和AG1478組。IL－8組顯著高于對照組、IL－8I組和IL－8＋IL－8I組(均P<0.05)，EGF＋AG1478組顯著高于EGF組和IL－8組( P<0.05)。結論： IL－8與EGF均能促進人黑素瘤細胞的增殖，它們之間可能具有相互協同作用，這種協同作用可能與AKT和MAPK等共同通路作用有關。
목적：：명학IL－8여표피생장인자( EGF)대흑소류세포증식적영향。방법：장흑소류세포A375분위9조：EGF조；EGF＋AG1478조；AG1478조；IL－8조；IL－8I조；IL－8＋IL－8I조；EGF＋ IL－8조；EGF＋IL－8＋AG1478＋IL－8I조급대조조。채용MTT법검측각조세포증식정황；면역인적법검측EG-FR、단백격매B、사렬원활화단백격매、린산화단백격매B、린산화사렬원활화단백격매、린산화표피생장인자수체적표체；실시형광정량취합매련식반응검측EGFR여IL－8R mRNA수평적표체。결과：EGF조흑소류세포증식、상술단백적표체화mRNA수평현저고우대조조、EGF＋AG1478조화AG1478조。IL－8조현저고우대조조、IL－8I조화IL－8＋IL－8I조(균P<0.05)，EGF＋AG1478조현저고우EGF조화IL－8조( P<0.05)。결론： IL－8여EGF균능촉진인흑소류세포적증식，타문지간가능구유상호협동작용，저충협동작용가능여AKT화MAPK등공동통로작용유관。
Objective:To determine the effects of IL-8 and epidermal growth factor ( EGF) on the prolif-eration of melanoma cell. Methods: The human melanoma cells A375 were divided into 9 groups: control group, EGF group, EGF combined AG1478 group, AG1478 group, IL-8 group, IL-8I group, IL-8 com-bined IL-8I group, EGF combined IL-8 group and EGF combined IL-8, AG1478 and IL-8I group. The pro-liferation of melanoma cell was detected by MTT. The level of EGFR, protein kinase B ( Akt) , mitogen activa-ted protein kinase (MAPK), phosphorylated protein kinase B (P-Akt), phosphorylated mitogen activated protein kinase ( p-MAPK) and phosphorylated epidermal growth factor receptor ( p-EGFR) were detected by western blot. The expression of EGFR and IL-8R mRNA levels were detected by qT-PCR. Results:The cell proliferation, the levels of proteins above and EGFR, and IL-8R mRNA levels in EGF group were higher than that in control group, EGF combined AG1478 group and AG1478 group ( P<0.05) . Those in IL-8 group were higher than that in control group, IL-8I group, IL-8 combined IL-8I group. Those in EGF combined IL-8 group was higher than that in IL-8 group and EGF group (P<0.05). Conclusion: IL-8 and EGF can pro-mote the proliferation of melanoma cells. There may be a synergistic effect between IL-8 and EGF, which may be related to the Akt and MAPK pathway.
