CAJ | 학술논문

调查分析273例HCV感染患者基因亚型构成，为临床HCV患者的抗病毒治疗方案选择提供依据，为HCV流行病学提供资料。收集武汉大学人民医院检验科自2011年3月至2014年5月273例HCV患者标本一代测序的HCVNS5B基因测序结果，并将所测的HCVNS5B基因核苷酸序列在线比对，确定其基因亚型。经在线比对后发现273例HCV患者共有4种基因型，分别是1型，2型，3型和6型；6种基因亚型分别是1a亚型3例（1.10%）、1b亚型208例（76.19%）、2a亚型41例（15.02%）、3a亚型3例（1.10%）、3b亚型12例（4.40%）和6a亚型6例（2.20%）。所收集的273例HCV分布情况和我国整体流行情况较一致，以1b亚型为主，其次为2a亚型，其他亚型少见。该分析为本地区HCV患者选择有效的个体化抗病毒治疗提供数据支持，为HCV的流行病学提供相关资料。
조사분석273례HCV감염환자기인아형구성，위림상HCV환자적항병독치료방안선택제공의거，위HCV류행병학제공자료。수집무한대학인민의원검험과자2011년3월지2014년5월273례HCV환자표본일대측서적HCVNS5B기인측서결과，병장소측적HCVNS5B기인핵감산서렬재선비대，학정기기인아형。경재선비대후발현273례HCV환자공유4충기인형，분별시1형，2형，3형화6형；6충기인아형분별시1a아형3례（1.10%）、1b아형208례（76.19%）、2a아형41례（15.02%）、3a아형3례（1.10%）、3b아형12례（4.40%）화6a아형6례（2.20%）。소수집적273례HCV분포정황화아국정체류행정황교일치，이1b아형위주，기차위2a아형，기타아형소견。해분석위본지구HCV환자선택유효적개체화항병독치료제공수거지지，위HCV적류행병학제공상관자료。
Objective To analyze genetic subtypes of HCV in 273 patients, and to provide evidence for clinical antiviral treatment options to HCV patients and provide data for HCV epidemiology. Method 273 HCVNS5B gene sequence results, since March 2011 to May 2014 in Renming Hospital of Wuhan University, were collected by generation sequencing assay. Then we compared the results of gene nucleotide sequence of HCVNS5B online, determined HCV genetic subtypes. Result After online comparison, we found that there were 4 genotypes in those 273 cases, namely type1, type 2, type 3 and type 6. And there were 6 genetic subtypes , namely 1a subtype 3 cases(1.1%), 1b subtype 208 cases(76.19%), 2a subtype 41 cases(15.02%), 3a subtype 3 cases(1.1%), 3b subtypes 12 cases(4.4%) and 6a subtypes 6 cases(2.2%). Conclusion The distributions of 273 cases were consistent with those of current China; 1b subtype was the main type, followed by 2a subtype, and the others were rare. These differences could provide a basis for antiviral therapy and offer information for HCV epidemiology in the area.